Koj puas paub tias lub siab ua kua zog chromataphy?

(HPLC) yog hom chromatography nyob rau hauv uas cov kua hloov theem hla dhau ntawm cov kem theem tas li nyob rau theem siab. HPLC yog cov cuab yeej analytical muaj zog siv los ntxuav, cais, txheeb xyuas, thiab seb txhua qhov sib xyaw ntawm kev sib xyaw.

High-siab ua kua chromatographyCov khoom siv muaj cov theem mobile, twj, txhaj tshuaj, cov kab sib cais, thiab cov ntsuas kev ntaus, thiab ntes. Cov qauv khoom siv tau txheeb xyuas los ntawm kev nqus cov theem ua kua (cov kuab) sib xyaw nrog cov qauv rau hauv ib kem. Txhua feem ntawm cov sib tov sib txawv sib txawv rau cov khoom siv nqus, sib cais cov cheebtsam ntawm cov qauv cov khoom siv thiab cia nws tawm ntawm lub sijhawm sib txawv. Ua ntej tshaj plaws, cov khoom uas khi zoo rau theem ua kua tawm los.
Tom ntej no, cov khoom siv uas khi zoo rau theem khoom tawm los. High-siab ua kua chromatography yog siv los ntsuas thiab ntxuav cov ntsiab lus ntawm ntau yam ntawm cov qauv khoom. Ua piv txwv, nws tuaj yeem siv rau hauv kev lag luam kev lag luam. Hauv kev lag luam no, HPLC yog qhov muaj txiaj ntsig heev rau kev ntsuas ntsuas qib ntawm cov tshuaj muaj ntau yam hauv qee cov ntaub ntawv.

Yuav luag 90% ntawm txhua High-siab ua kua chromatography Siv cov UV-ntes ntsuas ntsuas cov kev xav ntawm kev nqus tau spectroscopy. Qhov kev xav tau pom los ntawm npias-Lambert txoj cai: A = C L. Lub sijhawm ntsuas qhov kev tiv thaiv lub sijhawm ntawm ib lossis ntau qhov wavelengths. Muaj peb hom detectors.

Ib qho wavelength ntes nrog lub tshuab hluav taws xob ruaj khov, xaiv yoj xaiv nrog kev sib hloov ntawm cov hlua thiab txiv ntoo-ntes (txiv). IBSen Muaj cov txiv spectrometers uas yog daim ntawv nrawm tshaj plaws thiab feem ntau ntau tshaj plaws ntawm spectrometer nrog UV-detectors.

Yog tias koj muaj lus nug txog High-siab ua kua chromatography, lossis xav tauCov chromatography vials, Thov hu rau peb tam sim no!