High-kev ua si kua chromatography (HPLC) thiab ua kua chromatography-mass spectrometry (LCMS Thaum ob txoj hauv kev koom nrog kev sib cais thiab kev soj ntsuam ntawm cov tebchaw, lawv cov ntsiab cai, cov haujlwm, thiab cov ntawv thov sib txawv heev. Cov ncej blog no yuav sib piv HPLC thiab LCMS, tsom mus rau lawv qhov sib txawv tseem ceeb thiab qhov zoo ntawm txhua tus txheej txheem.

Lub ntsiab cai ntawm kev ua haujlwm

HPLC

HPLC yog cov txheej txheem chromatographic uas cais cov teb chaws sib cais raws li lawv cov kev sib cuam tshuam nrog lub chaw nyob ruaj ruaj thiab theem mobile. Nyob rau hauv HPLC, cov kua mobile phase yog nqus los ntawm ib kem nrog cov theem nyob ruaj khov, uas feem ntau yog tsim ntawm cov khoom lag luam me me ntawm silica lossis polymers. Thaum cov tshuaj sib tov tau qhia rau hauv cov kab ke, cov khoom siv tau sib cais raws li lawv muaj kev sib raug zoo rau theem nyob ruaj ruaj. Cov tebchaw uas sib cuam tshuam ntau dua nrog cov theem ruaj khov elutary elute ntau qeeb dua, thaum sib txuas uas sib cuam tshuam tsawg heev dua. Cov sib cais sib cais yog tom qab pom tau siv ntau yam detectors, xws li UV-Vis, Fluorescence, lossis Refactive Index Detalors.

Xav paub ntau ntxiv txog cov ntawv thov ntawm chromatography vials, thov khij cov lus no:15 Daim ntawv thov ntawm chromatography vials nyob rau hauv ntau teb

Lcms

LCMS sib xyaw ua ke ntawm kev sib cais muaj peev xwm ntawm HPLC nrog kev txheeb xyuas cov peev xwm ntawm huab hwm coj tau ntau. Hauv LCMS, cov qauv yog thawj cais siv HPLC, ib yam li hauv kev tso pa HPLC. Txawm li cas los xij, es tsis txhob raug kuaj pom los ntawm cov neeg ntes pom zoo, cov lus cog tseg muaj kev vam meej tau qhia rau hauv lub dav hlau loj. Lub loj spectrometer ionizes lub tebchaw thiab ntsuas lawv cov qauv cov ntsiab lus, muab cov ncauj lus kom ntxaws txog cov lus hnyav. Qhov kev sib xyaw ua ke ntawm kev sib cais thiab cov loj spectrometry muab ntau dua qhov kev paub ntau dua HPLC ib leeg.

Cais Misis

HPLC:HPLC siv kev kub siab kom thawb cov kua mobile theem los ntawm cov ntawv ntim ntim uas muaj cov theem nyob ruaj khov. Cov tebchaw tau sib cais raws li lawv cov kev sib cuam tshuam sib txawv nrog cov theem ruaj khov, uas ua rau muaj kev sib cais thiab ua kom muaj txiaj ntsig zoo.

LCMS:Lcms sib xyaw ua ke sib cais ntawm lub cev muaj peev xwm ntawm HPLC nrog lub peev xwm loj rau cov peev xwm ntawm cov khoom loj (MS). Cov kua chromataphy Cheebtsam cais tawm cov tebchaw, thaum lub teeb spectrometer ntsuas qhov loj-los piv txwv ntawm cov tsiaj.

Sensitivity thiab Xaiv

HPLC:HPLC muaj qhov zoo rhiab thiab xaiv tau, tshwj xeeb tshaj yog thaum ua ke nrog cov nuj nqis ua ke xws li UV-vis, lossis diode ntes ntes. Txawm li cas los xij, HPLC ib leeg yuav tsis muab tshwj xeeb rau qhov tshwj xeeb rau cov qauv nyuaj.

LCMS:LCMS muaj kev paub zoo dua thiab xaiv tau piv rau HPLC. Pawg Spectrometers tuaj yeem txheeb xyuas qhov tseeb thiab ntau qhov chaw sib txuas raws lawv cov kev sib piv loj-los them nyiaj, txawm tias nyob hauv cov nyom matrices. Qhov no ua rau LCMS tshwj xeeb muaj txiaj ntsig rau cov kab sib txuas thiab lees paub qhov tseeb ntawm cov tshuaj tsis paub.

Qauv npaj

HPLC:Qauv npaj rau HPLC feem ntau cuam tshuam nrog dmtrution, pom, lossis cov txheej txheem yooj yim. Cov qauv tsis tas yuav tsum tau evaporated, yog li HPLC yog qhov haum rau ntau yam ntawm cov tebchaw, suav nrog cov tshuaj polar thiab tsis muaj txiaj ntsig.

LCMS:Qauv npaj rau LCMS zoo ib yam li ntawd rau HPLC, tab sis yuav tsum tau cov kauj ruam ntxiv kom paub meej nrog lub loj spectrometer. Piv txwv li, cov tsis sib xws yog cov khoom sib ntxiv tuaj yeem tsim nyog txhim kho ionization thiab tiv thaiv one supression.

Cov Ntawv Thov

HPLC:HPLC tau dav siv rau kev tsom xam ntawm cov tshuaj, khoom noj khoom haus, thiab kuaj ib puag ncig. Nws yog qhov tshwj xeeb rau kev sib cais thiab cov nyiaj txiag sib xyaw ua ke, xws li cov tshuaj tsis zoo, cov tshuaj ntxiv zaub mov, thiab tshuaj tua kab tshuaj tua kab.

LCMS:Lcms muaj ntau cov ntawv thov, suav nrog:

Kws Tshawb Xyuas Kev Ntsuam Xyuas: Siv Cov Tshuaj Kho Tshuaj, Kev Loj Hlob, thiab Tswj Zoo.

Proteomics thiab cov metabolomics: siv los txheeb xyuas thiab ntsuas cov tshuaj muaj protein thiab metabolites hauv cov piv txwv txheeb xyuas.

Kev tsom xam ib puag ncig: siv los kuaj cov kab mob paug hauv dej, av, thiab cov pa taws.

Xav paub txog lub luag haujlwm ntawm cov ntawv pov thawj HPLC vials hauv kev txheeb xyuas cov qauv kev txheeb xyuas qhov tseeb? Nyeem kab lus no: Qhov tseem ceeb ntawm ntawv pov thawj HPLC vials rau kev txheeb xyuas qhov piv txwv tseeb

Tag

HPLC thiab LCMS yog ob qho tib si muaj zog tshaj tawm nrog lawv tus kheej qhov ua tau zoo thiab kev txwv. Qhov kev xaiv ntawm ob qho nyob ntawm qhov xav tau ntawm kev tsom xam, xws li rhiab, xaiv tau, qauv qauv, thiab muaj cov peev txheej.

HPLC yog qhov kev xaiv zoo rau kev tshuaj ntsuam txhua yam ntawm cov tebchaw uas paub yooj yim, qhov twg cov nqi thiab yooj yim ntawm kev siv yog qhov tseem ceeb yog qhov tseem ceeb. Lcms, ntawm qhov tod tes, yog cov txheej txheem uas nyiam tshaj plaws rau kev txheeb xyuas cov qauv nyuaj, txheeb xyuas cov ntsiab lus tsis paub, thiab kev ua tiav ultra-lw.

Raws li thev naus laus zis txuas ntxiv mus ua ntej HPLC thiab LCMS yuav ua txoj haujlwm tseem ceeb ntxiv hauv kev hloov pauv ntawm cov tshuaj aneverical thiab kom muaj txiaj ntsig zoo thoob plaws ntau yam kev siv.

Yav dhau los:

Lub ntsiab lus kawg rau 0.45 Micron Wyron Lim Lim Rau Koj

Tom ntej:

Txhua yam koj xav paub txog Headspace GC Qauv Npaj