Usoro nkwadebe maka GC-MS

Nnukwu Chromatography-jumprometry (GC-MS) bụ usoro nyocha dị ike nke ejiri iji nyochaa Valatile na ogige nchịkọta. Dabere na ọdịdị nke ihe nlele ma lekọta nyocha, enwere ike iji usoro dị iche iche iji kwadebe ihe nlele. Ihe ndị a bụ usoro a na-ahụkarị eji eme ihe nlele makaNnyocha GC-MSS:

Chọrọ ịmatakwu banyere ọdịiche dị n'etiti LC-MS na GC-MS, biko lelee isiokwu a:Kedu ihe dị iche n'etiti LC-MS na GC-MS?

1. Nkwadebe mmiri mmiri

A na-ejikarị usoro mmiri mmiri dị ka mmiri dị ala dị ka methanol, acetone, ma ọ bụ dichloromhane iji nweta ịta ihe dị ka 0.1 mg \ / mg \ / mg \ / mg \ / mg \ / mg \ / mg \ / mg \ / mg \ / mg \ / mg. Nke a na - eme ka nke ahụ dakọtara na sistemụ GC ma belata ihe ize ndụ nke ịmachi inlet.

FLTRRAPR :: Tupu nyocha, ihe nlere a kwesịrị ka iwepu ihe ndị ahụ nwere ike itinye aka na nyocha ahụ. A0.22 μm iyoa na-ejikarị ya.

Centrifugation: Maka ihe nlere nke nwere ike inwe solids, centrifugation nwere ike inye aka ikewapụ mmiri mmiri site na ihe ọ bụla na-agbanwe agbanwe tupu ịnyefe ya na vial.

2. Kwadebe nkwadebe siri ike

Mgbasa: A ga-enwerịrị ihe nlele siri ike na-adakwasị ihe dị ala na-acha odo odo. Tinye obere ego (ọka ole na ole) nke siri ike nye ihe mgbaze ma kpoo ọtụtụ oge iji hụ na mgbasa zuru oke.

Divatization: Maka ọkara nke na-agbanwe agbanwe ma ọ bụ polar nwere ike ịdị mkpa iji bulie elu na-eme ka ọ dị mma. Nke a na-agụnye nyocha kemịkalụ iji mee ka ọ bụrụ ihe anakpo ahụ na nyocha GC.

3. Nnyocha ndị isi

A na-eme ihe ngosi a, na usoro a kara aka nwere ihe atụ ahụ na mgbe niile okpomọkụ iji nyefee ogige na-agbanwe agbanwe na-agbasa n'elu ihe nlele ahụ. N'otu oge ruru nha, enwere ike sampled hedplis a maka nyocha nke na-ejikọ ikuku.

Dysic Headpace (Plage na ọnyà): Usoro a gụnyere gas na-aga gas na-aga gas site na nlele iji kwalite mmịpụta nke ihe na-agbanwe agbanwe. Usoro a na-abawanye na-abawanye ụba site na itinye uche na Vandatiles tupu nyocha.

Chọrọ ịmatakwu gbasara gịnị ka a na-eji lualpace na-eji chromatography ?, Biko lee akara a: Kedu ihe kpatara eji eme ihe nkiri ndị isi na Chromatography? Akụkụ 12

4. Usoro Ikpeazụ

Ihe microxtractions (spme): Spme na-eji akpati eriri ejikọtara ya na mmịpụta mmịpụta iji nweta nyocha site na mmiri ma ọ bụ gas. Usoro a na-enye ohere ịtụle nlele na-enweghị mkpa maka mgbatị ahụ ma bara uru karịsịa maka ogige na-agbanwe agbanwe.

Mwepu mmiri mmiri (LLE) na mmịpụta usoro siri ike (SPE): A na-eji ụzọ ndị a mee ka ọ dị ọcha site na iche na-egbochi ihe dị mgbagwoju anya na nyocha nke GC-MSS.

5. Ndụmọdụ maka ilekwasị anya

Nitrogen na-asacha: A na-eji usoro a etinye uche na sampless site na ịkwali na-efegharị n'okpuru iyi nke nitrogen, nke na-enyere aka belata ọtụtụ ndị na-enyocha.

Nlele Nkwadebe

Gbaa mbọ hụ na sozents niile eji na-agbanwe ma dabara adaba maka GC-ms; Ekwesịrị izere mmiri na mgbochi na-adịghị mma.

Ihe nlere aghaghi inwe acid ọ bụla siri ike, ntọala, nnu, ma ọ bụ ihe nnọchi ndị ọzọ nwere ike imebi kọlụm GC ma ọ bụ gbochie ya na nyocha ahụ.

Ihe nlele ikpeazụ kwesịrị ịbụ nke na-eme ka ọ dị mmavial vials iji gbochie ịtọpụ ihe site na plastik.

Maka ozi ndị ọzọ gbasara Val chromampler Vals Chromatography, zoo aka na edemede a: 2 ml Autosampler Vals maka chromatography

Mmechi

Ntinye aka nkwadebele dị mkpa maka nyocha nke GC-MS. Usoro nke ọ bụla nwere uru ya ma nwee ngwa ụfọdụ dabere na ọdịdị nke ihe nlele na onyinye zube. Site na iji usoro ndị a, ndị nyocha nwere ike melite izi ezi, mmetụta, na usoro nlekota nke gburugburu ebe obibi, nchekwa nri, na ọgwụ ọgwụ.