Nkwadebe na FLTRRAPR: na-akwado ịdị na Chromatography

Ndị na-asacha ngwaahịa dị ọcha, wepụ ya, ọ bụrụ na ọ dị mkpa, ma ọ bụrụ na achọrọ ya, ma ọ bụrụ na aMalize - na-agbasa ọnụ ọgụgụ dị nkọ na ntọala dị nkọ. Enweghi ebe a na-eme ka ọ dị elu na-abawanye elu, na-ebute ntọala, ma ọ bụ ihe mgbochi kọlụm.

1. Njehie & Matrix

Nsogbu

• Ihe ndabere baseline na-ebili na mkpọtụ na-eto eto obere obere.

• Ọnụ ọgụgụ a na-atụghị anya ya apụta site na adịghị ọcha.

• Ntụgharị uche dị n'etiti ogbugba.
  

  

Mkpatara

• Idobe na glowware: acid na-acha uhie uhie na-ezighi ezi; plastik nwere ike ịchichie chara acha.

• Nhicha nhicha: Chọpụta Organics na Soundsents na-eme ka ihe nkiri na-egbu egbu.

• Gburugburu ebe obibi na-akụ: lan lab ikuku na-eme mkpọtụ na ihe ngosi okwu na-eme ihe nlele.
  

  

Osisa

1. Uwe nhicha siri ike: Acid book → ultrasonic ọcha mmiri → Nitrogen akọrọ ga-akpọ clowware; ihe raara onwe ya nye maka plastik.

2. Akwukwo efu: Centrifuge na 3000rpm maka 10min ma ọ bụ jiri prefilter 7μμ nke ọma 0.22μm.

3. Gburugburu ebe obibi: Mee prep ke ube limimar na-agbadata mmetọ ikuku.
   
   

2.

Nsogbu

• Mkpu nrụgide na ntachi anya.

• Ọnụego mbelata, mpaghara ọnụ ọgụgụ.

• Ọkacha azụ na kọlụm.
  

  

Mkpatara

• Membrane na-eme ihe na-emegharị emegharị; Pvdf creeps na okpomọkụ dị elu.

• Ogo na-ezighi ezi na-ezighi ezi: 0.22μm finlers ọnyà mma ma belata nfe; 0.45μMm nwere ike imebi ihe na microcrys.

Osisa

1. Membrane-solvent matriks: Jiri mmanu pres \ /ce maka Asce (PH4-8), hydrophobic ptfe \ / pvdf maka uzo; Zere ịkpa cellulose na acid siri ike.

2. Họrọ Ogo Pore: Jiri 0.45μm maka usoro HPLC, 0.22μm maka UHPLC ma ọ bụ Chọpụta ọrụ; prefilter dị elu site na 5μm.

3. Nyochaa Δp: Usoro mgbali; Dochie nzacha mgbe alabasị na-abawanye> 15%.
   
   

   

3.

Nsogbu

• Nlele na-enyocha igwe ojii ma ọ bụ na-eburu ya.

• Ọhụrụ Baseline ma ọ bụ Plative Platives post-fltration.

• Belatara ka ọ gbakee.
  

  

Mkpatara

• Silent Ọsọ: thf na-aza ptfe, na-agbanwe nha na-aga.

• Ihe ndị a na-ekwu okwu ndị ọzọ: nylon na-acha acid nke na-ewepụta Oligomers nke na-abanye na 214nm.

Osisa

1. Ule nnwale: Sook nzacha na-eme ihe mgbaze maka 24h na 25 Celsius C; Nyochaa maka mgbanwe ma ọ bụ mgbanwe ibu.

2. Nnwale LC-MS: Tulee mkpokọta ion chromatogram nke enter vs. splele.

3. Jiri ihe nzacha dị ala: Ultrasonic-welded ptfe ma ọ bụ pes na-enweghị adresives.
   
   

Sistemụ dị mma: Nchọgharị-Nlegharị anya

Ozize

Standay sọks maka ihicha, nzacha, na mempbrane nhọrọ iji gbochie njehie.

Nleba anya

Dekọọ ọnụego na-asọpụta, azụ azụ, na oghere na-enweghị oghere iji chọpụta na ọ na-achọpụta.

Mmaaki

Nọgide na-enyocha nyocha osisi maka ngwa ngwa maka njikọta ngwa ngwa na ndozi.

"A na-amalite usoro nyocha nke kacha nta."