Know mara maka ọgwụgwọ silanization nke hplc vial?

Silinization nke HPLC VIalsbụ usoro dị oke mkpa nke emere iji meziwanye arụmọrụ chromatographic site na belata nrasion nke nyocha na-acha uhie uhie. Ọgwụ a bara uru karịsịa maka nyocha nke ọma, ọkachasị mgbe ị na-eme nyocha dị ala, dịka ihe nlele ọ bụla nwere ike imetụta nsonaazụ ya. Isiokwu a na-enye nkọwa zuru oke banyere usoro Silylation, uru ya, uru ya, uru ya, na nchebara echiche maka iji mmiri egwu Chromatography (HPLC).

Kedu ihe na-eme?

Synanization na-agụnye itinye onyinye nke ime ụlọ na-eme nke ọma. Usoro a na-emebi hydroxyl (Silanol) na iko, nke a na-ama iji soro na nyocha dị iche iche, karịsịa polar molecules. Site na iji masking salting, slinanization nke ukwuu na -ebelata mgbasa ozi nke nyocha nke nyochara vial na nsonaazụ nyocha dị elu. Na HPLC VIals, ọgwụgwọ a na-ebute silanol (Si-oh) dị n'ime ụlọ nke borosilicate iko. Ndị otu a hydroxy nwere ike iso na nyocha polar, na-eduga n'ịtụle mgbasa ozi na ọnwụ n'oge nyocha.

Usoro silinanization

Usoro silization na-emetụta usoro ndị a:

Nhicha: Nke mbụ, hichaa iko ahụ nke ọma site na iji ihe na - eme ihe ga - eme ka ọ bụrụ acetone iji wepu ụdị mmerụ ahụ nke nwere ike igbochi ya na olization.

A na-ewebata oge silone: ​​Ihe okike Methyl na-eme ka a mata na ọnụ ala nke vial site na Vapor. Onye na-elekọta ya na ndị otu Hydroxy na-etolite oyiso agbụ hydrophobic nke na-abaghị uru na-emecha.

Njikọ Colone na-emeghachi omume na otu Hydroxy na iko dị elu iji mepụta oyi akwa, na -ebelata mmeghachi omume na igbochi ịgbaso mgbidi vial.

A na-agwọta: A na-agwọta karama agwọ ahụ iji hụ na siline na-ejikọ eriri dị mma na ihu iko ahụ, si otú ahụ na-abawanye na mgbatị na ọnọdụ ndị ọzọ na-ezute na Chromato.

Ihe si na ya pụta bụ vial na elu elu nke belata mmekọrịta ya na polar dị iche iche, na-eme ka mgbake nlele na nyocha.

Uru nke Silinization

Na-eji melanized hplanized Vals na-enye ezigbo uru:

1. Meziwanye mgbake nlele

Vals na-emegharị ahụ nwere ike belata ndọnye nke nyocha. Nke a dị ezigbo mkpa maka nyocha ndị dị ala, ebe ọbụlagodi ntakịrị ihe nlele nwere ike imetụta nsonaazụ ị ga-arụpụta. Site na igbochi mgbasa ozi, vials siys na-agbake na mgbake kachasị nke nyocha.

2. Meziwanye eziokwu

Ọgwụgwọ a na - eme ka ọ pụta ìhè na nsonaazụ ya na - emetụta ogo elu, si otú a na - eme ka izu ike dị oke mkpa maka nyocha dị oke mkpa.

3. Ngụkọta na nyocha dị iche iche

Vials na-elilized dị mma maka ọtụtụ ngwa dị iche iche, gụnyere ndị metụtara polar na ogige hydrophobic. Ha na-adabara ọgwụ mma maka ọgwụ, ndị isi na ihe ndị ọzọ nwere mmetụta ndị ọzọ nwere ike ijikọ ndị na-enweghị isi na-emekọrịta ihe.

4. Ndụ LELF

Usoro sọks nke vapor na-emepụta mkpuchi mkpuchi siri ike nke na-ejikwa ihe ha na-eme. Colanized finanized na-akwụsi ike ogologo oge ọbụlagodi mgbe e kpughere mgbazi dị iche iche ma dịkwa mma maka nchekwa ogologo oge.

Ngwa nke HPLC VIals

Enwere ike iji Hollanized Hilly Vals na mpaghara dị iche iche:

1. Nyocha ọgwụ

Na ụlọ ọrụ ọgwụ, a na-eji vials kelenized maka:

Nnwale nkwụsi ike: Na-ejide na usoro ọgwụgwọ ahụ na-akwụsi ike n'oge nchekwa na nyocha.

Nyochaa nyocha: na-enyere aka ịta ọgwụ ịta ọgwụ n'ụzọ ziri ezi na nhazi.

2. Nnwale gburugburu

Vials ndị a dị mkpa maka nlekota gburugburu ebe obibi:

Nnwale dị mma mmiri: Nyochaa mmiri mmiri maka mmerụ ahụ mgbe ị na-ebelata mfu Admirption.

Nyochaa ala: Kwadebe ala extracts maka nyocha kemịkalụ na-enweghị ngụkọta iche.

3. Nlere nchekwa nri

Na ngwa nchekwa nri:

Nyochaa nyocha nke ọgwụ: Gbaa Nchọpụta ihe dị mma nke ọgwụ na-egbu egbu na nri site na igbochi ihe nlele anya n'oge usoro nyocha.

Nnwale ọdịnaya ọdịnaya: Nyochaa nri nri maka ọdịnaya nri na-enweghị ndabichi site na mkparịta ụka vial.

4. Nchọpụta na mmepe

Na ọnọdụ nyocha:

Nkwadebe sample: na-enye nchekwa nchekwa nke nchekwa maka ụdị nlele dị iche iche tupu nyocha.

Ngwa nnwale: nnwale ndị na - eme ka ihe dị iche iche chọrọ ọnọdụ njikọta.

Akpachapụ anya mgbe ị na-eji vials

Ọ bụ ezie na vials elized na-enye ọtụtụ uru, enwere ụfọdụ ihe dị mkpa iji mara:

1. Họrọ ụdị mara mma mara mma

Ọ dị mkpa ịhọrọ ụdị vial mekanized dị mma maka mkpa ngwa gị. Ihe ndị dị ka Sampy Mpịakọta, ndapụta na uche dị mma kwesịrị iduzi nhọrọ.

2.

Ọgwụ kwesịrị ekwesị dị mkpa iji nọgide na-eguzosi ike n'ezi ihe nke elu ndị siliki:

Zere ịkpọtụrụ gị na ime ụlọ iji gbochie mmerụ ahụ.

Yiri uwe elu dị ọcha mgbe ị na-ejikwa vials iji belata mbufe nke nsị.

3. Ngụkọta na sons

Ọ bụ ezie na elu silanaces na-eguzogide ọtụtụ solvents, ọ dị mkpa ịchọpụta ndapụta na kemịkal dị iche iche eji eme nyocha ma ọ bụ enweghị ọrụ.

Ngwọta ọzọ

Ọ bụ ezie na olinaization dị irè, enwere nhọrọ ndị ọzọ dị ka ebelata ọrụ elu (RSA). Ezubere RSA Vials iji nwee ọrụ dị ala ala dị ala ma ghara ịdabere na colane comesions. Usoro a nwere ike ịnye nsonaazụ ndị ka ukwuu maka ngwa ụfọdụ ebe slanization ọdịnala nwere ike ịda ada n'ihi oge.

Ọ bụrụ n'ịchọrọ ịmatakwu banyere HPLC VILC VILC, Biko pịa ederede a:"Ngosipụta nke siliconized hplc vials"

Silanization nke Vall Viats bụ usoro dị mkpa na-eme ka nyocha nyocha site na ibelata nyocha nyocha na ịbawanye na mgbake nlele na mgbasawanye. Site na-agbada ime ụlọ na-egbochi oke iko, ọgwụgwọ a na-enweta ezikwute, nke a pụrụ ịdabere na ya n'ọtụtụ ngwa, nnwale gburugburu, nchekwa nri, nchekwa nri, na ntọala nyocha. Site na ịghọta uru na nchedo ojiji nke kwesịrị ekwesị metụtara ya na Vals elanized, nwere ike ime ka nyocha ha nyocha ka ọ na-eme nyocha ka ọ dị mma ma na-agbanwe oke na usoro nhazi ya. Dị ka teknụzụ Silenanization na-aga n'ihu, ndị na-eme nchọpụta nwere ike ịtụ anya na-aga n'ihu na arụmọrụ Valy na itinye aka na ọzụzụ sayensị.