Kedu ihe kpatara GC-MS ji anwale ọgwụ?

A na-amata na gas chyromatography-jumpleTometry (GC-MS) a na-ahụ maka nyocha nke ọma maka nyocha nyocha ọgwụ, ọkachasị n'ọhịa nke ụlọ ọgwụ na nke ụlọ ọrụ. Ikike ya inye ihe ziri ezi, na nsonaazụ ndị a pụrụ ịdabere na ya na-eme ka ọ bụrụ usoro ịhọrọ ọgwụ na-achọpụta ọgwụ na metabolite ha na metabolite ha na ihe nlere ndu ha. Blọọgụ a ga-enyocha ihe kpatara iji GC-m maka nyocha ọgwụ, na-elekwasị anya na uru ya, ụzọ, na ngwa.

Chọrọ ịmatakwu banyere ọdịiche dị n'etiti LC-MS na GC-MS, biko lelee isiokwu a: Kedu ihe dị iche n'etiti LC-MS na GC-MS?

Methology GC-MSS

GC-MS na-ejikọ usoro nyocha abụọ: gas chromatography (GC) na Mass Stiattometry (MS).

Chromatography: Na ọkwa mbụ a, ihe nlele a na-enye ma kewapụ ya na kọlụm ndị na-eji kọlụm. Nkewa na-adabere na isi mmiri na potarity nke ogige, na-enye ohere maka nkewakọta nke ọma.

Mass Stiattometry: Ozugbo ihe mejupụtara ya, a na-ewebata ha n'ime oke. N'ebe a, a na-eme nyocha na nke a na-eme nyocha na-adabere na ọkwa ha. Usoro a na-ewepụta ụdị dị iche iche dị iche iche maka ogige nke ọ bụla, na-enye ma data ma na-ahụkarị.

Usoro ụzọ abụọ a na-enye ohere maka njirimara zuru oke nke ihe nlele, na-eme GC-MS kachasị mma maka ule ọgwụ.

Uru nke GC-MS na nnwale ọgwụ

1. Mmetụta dị elu na nkọwapụta

Otu isi ihe kpatara eji GC-MS na nnwale ogwu bu ihe di elu:

Nchọpụta itinye uche: GC-MS nwere ike ịchọpụta oke ọgwụ ọjọọ nke ọgwụ, na-abụkarị nanogram \ / ML. Ike a dị oke mkpa na ntọala ụlọ ọgwụ, ebe ndị ọrịa nwere obere ọgwụ ma ọ bụ metabolite.

Nchọpụta akọwapụtara nke ọma: Mass dị iche iche na-enye ozi zuru ezu banyere usoro molecular nke ogige, na-enyere njirimara akọwapụtara ọbụna n'etiti ihe owuwu. Nkọwa a na-enyere aka belata isi ihe ndị dị egwu nke nwere ike ime na usoro nyocha ndị ọzọ.

2. Ike nyocha

GC-MS nwere ike ihuenyo maka ọtụtụ ihe:

Nnwale ọgwụ ọgwụ: Teknụzụ ahụ nwere ike n'otu oge nyocha ọtụtụ ọgwụ na metabolites ha n'otu ihe. Ike a zuru oke na ọgwụ na-egbu egbu, ebe enwere ike ikpughere ndị ọrịa na ọtụtụ ihe dịgasị iche iche.

Na-eme mgbanwe maka ihe ndị ọhụrụ: Dịka ọgwụ ọhụụ na-abịa n'ahịa, a nwere ike itinye ọgwụ ndị a na usoro nnwale maka GC-MS site na imelite usoro usoro ma ọ bụ na nchekwa data eji maka njirimara.


Know mara ihe dị iche n'etiti HPLC VIals na GC VIals? Lelee ederede:Kedu ihe dị iche n'etiti HPLC VIals na GC Vials?

3. Nnwale nkwenye

Ọ bụ ezie na nnwale nyocha mbụ, dịka immuniashas, ​​nwere ike igosi ọnụnọ nke ọgwụ, ha anaghị egosi na:

Nyochaa nkwenye: Enwere ike iji MPS-MS dị ka ule nkwado mgbe nyocha izizi. Enwere ike nyochaa nsonaazụ dị mma site na immunoasseys na nyocha, na-enye ihe akaebe dị mkpa maka iwu iwu ma ọ bụ nyocha.

Nchịkọta nke iwu: Na ntọala Forensic, ụlọ ọrụ na-achọkarị nnwale na-achọkarị nnwale site na usoro dị ka GC-MS iji hụ na izi ezi na ntụkwasị obi nke nyocha na ntụkwasị obi.

Ngwa nke GC-MS na nnwale ọgwụ

1. Ntughari ogwu

N'ime ụlọ ọrụ na-egbu egbu na-egbu egbu, a na-ejikarị GC-MS nyocha nyocha mmamịrị maka ọgwụ maka ọgwụ:

Ọgwụ nke ọgwụ ọjọọ na nke na-egbu egbu: ọ na-arụ ọrụ dị mkpa n'ịtụle ndị ọrịa nwere ike ịgbanwe ọnọdụ uche ma ọ bụ nsị. Site na iji kọwaa ihe ndị dị ka mmamịrị, ụlọ ọgwụ nwere ike ime mkpebi ọgwụgwọ.

Jiri ọgwụ na-enyocha ọgwụ: A na-ejikwa ọgwụ GC-MS mee ihe na-enye ọgwụ, na-ejide na ndị ọrịa na-anara ọgwụ dị ka ndị na-eduzi ma ọ bụ na-emejọ ha.

2.. Ngwa nyocha

A na-eji GC-MS ọtụtụ ihe na-amụ ọgwụ Metabolism na ọgwụ Plashọransị:

Nyochaatabolite: Ndị na-eme nchọpụta na-eji GC-MS nyochaa metabolites mepụtara mgbe ị na-eme ọgwụ nchịkwa, nke na-enyere aka ịghọta etu ọgwụ a na-ahazi ahụ.

Mmepe nke usoro nyocha nke ọhụrụ: Mgbanwe nke GC-MS na-enye ndị ọrụ nchọpụta iji zụlite ụzọ ọhụụ ma ọ bụ matriki, si otú a na-eme ka ikike nyocha nke nyocha ọgwụ.

Mmechi

Nnukwu Chromatography-ukarmetry (GC-MS) abụrụla teknụzụ ọla edo maka nyocha ọgwụ n'ihi oke echiche ya, ịkọwapụta, na ikike nyocha ya. Ikike ya inye nsonaazụ nke mmechi na-eme ka ọ dị mkpa na nesicology abụọ na nyocha nke nyocha. Dị ka ọgwụ ọhụrụ na-aga n'ihu na-apụta ma na-etolite, mmeghari nke GC-MS na-ahụ na ọ na-anọgide na-ebute ụzọ nyocha ọgwụ na-eme ọgwụ.

Site n'iji teknụzụ MS nke ọma, enwere ike ịkwalite ikike nyocha ha mgbe ị na-azụta ezi uche na nsonaazụ dị oke egwu na nchekwa nchekwa na iwu.


Maka ozi ndị ọzọ gbasara Val chromampler Vals Chromatography, zoo aka na edemede a: 2 ml Autosampler Vals maka chromatography