He aha i kore ai to Whakaora: He Aratohu a te Tohunga Taiwhanga mo nga Vials HPLC

I roto i te ao nui-nui o te wetewete ultra-trace (ppb\/ppt taumata), ka aro nui tatou ki te matū o te pou me te tairongo o te papatipu, heoi ka warewarehia te mata tuatahi i pa ki a maatau tauira: te pakitara ipu. Mo te tangata e whakahaere ana i te UHPLC-MS\/MS i roto i te proteomics, i te paitini haumanu ranei, ehara i te mea he ipu noa te ipu karaihe paerewa—he mahanga matū tera ka taea te whakakore i o hua i mua i te werohanga tuatahi.

Kua kite pea koe i nga tohu: he 20% te paheketanga o te waahi teitei na te mea i noho tetahi tauira ki roto i te autosampler mo te po, he porangi ranei "nga tihi wairua" e kore e noho. Ko te nuinga o nga wa, ehara i te mea nau te tangata hara; ko te silanol kinetics kei runga i to mata karaihe.

1. Te Adsorption Headache: He aha te Polar Analytes "Ka ngaro"

Ko te karaehe borosilicate paerewa, ahakoa teiteiUSP Momo 1 Karaehe, kei tawhiti atu i te koretake. Ko te mahi hangahanga ka waiho te mata e ngoki ana me nga roopu Silanol tauhohe (Si-OH).

• Te Rore Whakawhiti Katote: I te pH> 3, ka whakahekehia nga silanols ka kino te utu. Mena kei te mahi koe me nga raau taero taketake, peptides ranei, ka piri tonu ki te pakitara karaihe kare rawa e tae atu ki to kairapu.

• Te Hiko o runga: Ahakoa he iti te pH, ko te hononga hauwai i waenga i te karaihe me to tauira ka pa te wehitihi hiku. Kaore koe e kite i te tino aro; Kei te kite koe i nga mea e toe ana i muri i te ipu "ka tapahia."
  
  

2. Vapor-Phase Silanization: Ko te Wehenga Tuturu anake

Warewaretia nga ipu "wai-toua" iti. He maha nga wa ka "puddles" te paninga wai ki raro o te ipu—i te waahi tonu e noho ana o rita iti o te tauira.

I Aijiren, ka whakamahia e matou te Whakaaetanga Kohu Maama (CVD). I whiua e matou te karaihe ki te hau organosilane i roto i te korehau i te wera nui.

• Ko te "Kahu Invisibility": Ko tenei tukanga ka hanga i te here kovalent tuturu, ka hanga i te whakangungu rakau hei aukati i to tauira kia kore e "kite" i te kurupae karaihe reactive.

• Zero Bleed: Koinei te take ka tohe nga tohunga ngaioVials HPLC Silanised—Koinei anake te huarahi ki te whakarite ko te tauira ka mahia e koe he rite tonu te tauira ka werohia e koe.
  
  

3. Raraunga Tuturu-Ao: LC-MS\/MS Recovery

I roto i te tātaritanga tohu, ko te ōwehenga Waitohu-ki-Haruru (S\/N) te mea katoa. Ko nga ipu iti-paerewa e kore e kai noa i to tohu; ka turuturu nga katote e whakakino ana i to turanga.

Lab Pro Tip: Ma te whakamahiLC-MS\/MS mo te whakamatautau tarukino? Ko te Silanization to parepare pai ki te konutai (Na+) nga taapiri e whakararu ana i to hiranga me te pehi i to tohu katote.

Ko te karaehe karekau i rongoatia ka rewa nga katote whakarewa alkali. Mo nga tātari tairongo peneicatecholamine hākawa ranei, ko tenei nekehanga ka tere te whakaheke. Mena ka noho to tauira ki te rarangi, kei te tunu kai i roto i tana ake taiao kawakore. He pai te hiri a te Silanitanga i te kopu karaihe.

Kare e hiahia ana koe ki te karaihe silanized mo ia oma oma. Engari me tino hiahia koe ki te:

• He < 1 μg\/mL te kukū: He whakamate nga mate urunga ki enei taumata.

• Kei te whakamahi koeLC-MS Vials: Hei whakaiti i nga tihi wairua me te whakakore katote.

• Te whakamahiNga Whakauru-Moroiti: He nui te ōwehenga mata-ki-rōrahi i konei—ki te kaha te karaihe, kua ngaro to tauira.

• Pūranga me4mL Pupuri Puka: Ko te whakapumautanga mo te wa-roa e hiahia ana kia noho kore matū katoa.
  
  

Ko te Raina Raro o Aijiren

Kare noa matou i hanga he ipu; i hangaia e matou he taiao pumau mo to matū.

1. 2mL Vials Silanized: He mea hanga tika mo Agilent, Waters, me Shimadzu.

2. Te Whakakore Whakamana: Kore te siloxane ka toto ki to wahanga pūkoro.

3. Kua Whakamanahia te Hydrophobicity: Ko ia puranga he koki whakapiri-whakamatautauhia kia rite tonu te kapinga.
   
   

Kati te Ngarohia o Tauira - Tonoa he Kete Vial Kore Utu Karekau Inaianei