Aug. 30th, 2024
Kuchita masewera olimbitsa thupi kwa ma chpomatography (HPLC) ndi njira yofunika kwambiri yomwe imagwiritsidwa ntchito mu laboratories m'minda pafupifupi mankhwala, sayansi yachilengedwe, ndi chitetezo cha chakudya. Mkati mwa HPLC World, pali magulu awiri akulu: katswiri wa HPLC ndi PRPLC. Ngakhale maluso onsewa amagwiritsa ntchito mfundo ngati izi popatula mankhwala, amagwiritsidwa ntchito pazithunzi zosiyanasiyana ndipo amakhala ndi magawo osiyanasiyana ogwirira ntchito. Blog iyi ifufuza zosiyana pakati pa HPLC yowunikira komanso yopereka, kuphatikiza cholinga chawo, kuchuluka kwake kwa opaleshoni, zida, ndi ntchito.
Ndikufuna kudziwa zambiri za HPLC Vials, chonde onani nkhaniyi: Encyclopedia ya HPLC Vials
Kuzindikira HPLC HPLC
Tanthauzo ndi Cholinga
HPLC HOPLC imayang'ana makamaka pakulekanitsa ndi kudziwitsa anthu mosiyanasiyana. Cholinga choyambirira ndikupeza chidziwitso chokwanira komanso chochuluka chokhudza zinthu zomwe zilipo mwa kusakaniza. Kusanthula HPLC nthawi zambiri kumagwiritsidwa ntchito:
Kusanthula koyenerera: Kuzindikira zinthu zomwe zimapangidwira.
Kusanthula Kwakukulu: Kudziwitsa kuchuluka kwa gawo linalake.
Kuchuluka kwa opareshoni
HPLCILC HPLC imagwira ntchito pang'ono, nthawi zambiri zimakhudzana ndi mitundu yamagetsi yolumikizira. Mavoliyumu omwe amagwiritsidwa ntchito nthawi zambiri amakhala mu 1 mpaka 100 μl, amalola zitsanzo zingapo kuti zisanthulidwe kamodzi. Mlingo wocheperako ndi wabwino pakuyesa kwa mayesero ndi kuwongolera kwapadera.
Kukula kwake ndi kukula kwa tinthu
Ming'alu yomwe imagwiritsidwa ntchito powunikira HPLC nthawi zambiri imakhala yaying'ono m'mimba mwake, nthawi zambiri pafupi 4.6 mm, ndipo ili ndi tinthu tating'onoting'ono toyambira (3-5). Kusintha kumeneku kumalola kuti kusinthika kwakukulu ndi kupatukana bwino kwabwino, koyenera kusanthula mwatsatanetsatane.
Njira zodziwika
HPLC HPLC imagwiritsa ntchito njira zosiyanasiyana zodziwikiratu, kuphatikizapo UV-wowoneka, flurescence, ndi spectrimery. Kusankhidwa kwa cholembera kumadalira mtundu wa chiwerewere komanso zofunikira pakuwunika. Zowunikira zosawonongeka nthawi zambiri zimagwiritsidwa ntchito kuonetsetsa kuti chitsanzo chingasanthule popanda kutayika.
Ndikufuna kudziwa mayankho 50 onena za HPLC Vials, chonde onani nkhaniyi: 50 mafunso ambiri omwe amafunsidwa kawiri pa HPLC Mbale
Kumvetsetsa Kupereka HPLC
Tanthauzo ndi Cholinga
Komabe, HPLC, ikufuna kupatukana ndi kuyeretsa mankhwala kuchokera kwa osakaniza. Cholinga chachikulu ndikupatula kuchuluka kokwanira kwa zomwe mukufuna kuwunika kapena kugwiritsa ntchito. Pakhala zotsatila
HPLC imagwiritsidwa ntchito kawirikawiri pamapulogalamu otsatirawa:
Kupatula kwapadera: Kuchotsera ndi Kuyeretsa kwazinthu zina za kafukufuku kapena mafakitale.
Kukonzekera zitsanzo: Kukonzekera zitsanzo za kusanthula kotsatira kapena kapangidwe kake.
Kuchuluka kwa ntchito
HPLC yolembedwa imachitika pamlingo wokulirapo kuposa katswiri wa HPLC. Mafayilo azitsanzo amatha kukhala m'malilili ochepa mpaka malita angapo, kutengera zokolola zomwe zikuyembekezeredwa zomwe zingachitike. Kukula kwakukuluku kumathandizira kuti kuchuluka koyeretsedwa kusonkhanitsidwa kuti mugwiritse ntchito.
Kukula kwake ndi kukula kwa tinthu
Ming'alu yomwe imagwiritsidwa ntchito pokonzekera HPLC ili yokulirapo, imayamba kuyambira 20 mm mpaka 100 mm kapena wamkulu m'mimba mwake. Ming'aluyo imadzaza ndi tinthu akuluakulu okhazikika (nthawi zambiri 10-20 μm) kuti muwonjezere mitengo yotsika ndikugwiritsa ntchito mavidiyo akulu. Mapangidwe awa amathandizira kuchepetsa kupsinjika ndikukwaniritsa bwino zinthu.
Njira zodziwika
Panjira yopereka HPLC, kuona njira zitha kusiyanasiyana ndi zomwe zimagwiritsidwa ntchito pofufuza HPLC. Ngakhale zojambula zopanda madzi ndizofala, machitidwe omwe amaperekedwa nthawi zambiri amagwiritsa ntchito zojambula zomwe zimatha kutolera zigawo zozikika. Izi zimathandizanso mapangidwe apadera oti azikhala okhaokha monga akulerera kuchokera pamunsi.
Mukufuna kudziwa zambiri za kugwiritsa ntchito Mipata ya chromatography, chonde onani nkhaniyi:Njira zisanu zoyeretsa za HPLC Vials
Kusiyanitsa kwakukulu pakati pa zowunikira ndi zopereka hplc
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Kaonekedwe
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Katswiri hplc
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PANGANI HPLC
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Cholinga
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Kusanthula Kwathunthu ndi Kuchulukitsa
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Kudzipatula ndi kutsuka kwa zinthu
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Kuchuluka kwa opareshoni
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Scal-Scal (μl voliyumu)
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Lalikulu-lalikulu (ml to voliyumu)
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Mitengo ya Column
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Maondo ang'onoang'ono (4.6 mm)
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Mainchesi akulu (20-100 mm)
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Kukula kwa tinthu
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Tinthu tating'onoting'ono (3-5 μm)
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Tinthu tating'onoting'ono (10-20 μm)
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Mitengo yoyenda
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Mitengo yotsika yotsika (0.1-5 ml \ / min)
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Mitengo yokwera kwambiri (10-200 ml \ / min)
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Njira zodziwika
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Zojambula Zowonongeka (UV, Ms)
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Zopereka za Fraction zotengera chithunzi
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Kusankha pakati pa zowunikira ndi zolipirira HPLC
Mukamasankha pakati pa zowunika ndi HPLC, lingalirani zinthu zotsatirazi:
Cholinga cha kusanthula: Dziwani ngati cholinga choyambirira ndi chokwanira komanso chofufuzira kapena kupatula zinthu zina zogwiritsira ntchito.
Kuchuluka kwa zitsanzo: sinthani kuchuluka kwa zitsanzo zomwe zilipo. Ngati zitsanzo zochepa zilipo, katswiri wa HPLC ikhoza kukhala yoyenera. Komanso, ngati zitsanzo zambiri zimafunikira, HPLC yoyambirira ndi chisankho chabwino.
Kufunika Kuyera ndi Kuchulukitsidwa: Ganizirani za kuyera komwe kumafunikira ndi zokolola za chandamale. HPLC yolembedwa iyenera kukulitsa kuchira kwapakatikati, pomwe katswiri HPLC imayang'ana mwatsatanetsatane zomwe zimapangidwira pazomwe zimachitika.
Zida ndi zida: kuwunikira zomwe zilipo, kuphatikiza zida ndi bajeti. Makina a HPLC amakhala okwera mtengo ndipo amafuna ndalama zambiri mu zida ndi ndalama zogwirira ntchito.
Kodi amakonda kunena za HPLC zitsanzo kukonzekera? Onani nkhaniyi:HPLC zitsanzo zokonzekera bwino zotsatila bwino.
Mapeto
Onse owunikira ndi omwe ali ndi HPLC ali ofunikira maluso ofunikira m'munda wa chromatography, ndipo aliyense amagwiritsa ntchito zosiyanasiyana kutengera zosowa za ofufuza ndi makampani. Ngakhale kuwunika HPLC ndi yabwino kuwunika mwatsatanetsatane ndi kuchuluka kwa mankhwala, HPLC yoyambirira ndikofunikira kuti musunge ndi kuyeretsa zina zowonjezera mapulogalamu ena. Kuzindikira kusiyana pakati pa njira ziwirizi kungathandize ofufuza kuwonetsa zisankho zanzeru, yesetsani mapangidwe awo, ndipo mupeze zotsatira zolondola komanso zodalirika pakuwunika kwawo. Monga ukadaulo wa HPLC ikupitiliza kupita patsogolo, njira zonse zopezera komanso zopindulitsa zimakhala gawo lofunikira kwambiri kafukufuku wopambana wa asayansi.
Achizungu
Zauchina
