Momwe Mungasankhire Mbale Za HPLC

Mu kuyesa kwachilengedwe, ogwira ntchito labotale nthawi zambiri amakumana ndi mavuto a kusankha kuyesa kovuta. Kugwiritsa ntchito vaimp yolakwika kungayambitse zotsatira zoyipa. Zitha kuwononganso chitsanzo kapena chromatophi.

Pali mitundu yambiri ya Mbale Zazitsanzo, kodi tiyenera kusankha motani? Nkhaniyi ikusonyezani momwe mungasankhire Mbale. Kuchita izi kudzakuthandizani kulondola kwanu kuchiyeso cha chromatographic.

Kusankha Vual Autosambir Volial ndikofunikira kuti muwonetsetse zolondola ndi zodalirika za zotsatira zanu:5 Malangizo ayenera kuganizira posankha vial ya autosambir.

Mitundu ya HPLC Mbale

Titha kulembera ma rium azomwe ali ndi matumbo awo, pakamwa pa botolo, komanso mawonekedwe amkati. Mitundu yosiyanasiyana ya phwendes ndi yoyenera mayeso osiyanasiyana.

Malaya
Musanamvetsetse nkhaniyo, choyamba choyamba kumvetsetsa mzere wokulirapo. Kukula kwa kukula ndikusintha kutalika kwa kutentha kulikonse. Kuchulukitsa kotsika kumatanthauza kuti galasi litha kupirira kutentha kwambiri. M'mawu, ndi: kuthekera kwagalasi kuti tithane ndi kusintha kwa kutentha kwa kutentha. USP (United States Farmacacopea) imasinthanitsa galasi la nkhumba. Zimachokera pamadzi ake.

Tikudziwa mzere wowonjezera wagalasi. Chifukwa chake, kusankha zinthu kuti zikhale zosavuta zitsanzo zosavuta.

1. Tsamba la USP 1, kalasi ligalasi 33 Borosil Boga ndi galasi la mankhwala. Amagwiritsidwa ntchito kwambiri mu labotore, makamaka kwa chromatoptory. Magalasi amagalasi makamaka ndi opangidwa ndi silikicon ndi okosijeni, potengera ndalama za boron ndi sodium. Imakhala ndi kusungunuka kochepa komanso mzere wowonjezera wa 33.

2. Tsamba la USP 1, kalasi B 51 siyicon ndi mpweya. Imakhala ndi boron yaying'ono, sodium, ndi zina zambiri zalkali kuposa kalasi galasi. Komabe, imatha kukwaniritsa zosowa za labotale. Zogwirizana za kukula kwa mzere ndi 51.

3. Magalasi kapena odetsa kapena odetsedwa ndi mtundu umodzi wamagalasi. Zakhala zikuyikidwa chifukwa chokuta pansi ndi silanes. Hydrophobic ndi inert ndi galasi lagalasi. Zimakhala zothandiza posungira nthawi yayitali, mankhwala ophatikizira a P P P P P P P P P P P P P P P P PER.

4. Mtundu wa USP ndi NP Sodium-calcium galasi sinagonjetsedwe. Sakugwirizana kwenikweni kuposa galasi la ma borosil.

5.Polypropylene (ma pp) zinthu.

Mtundu wamakamwa
1.

Mtundu wa Thupi la Mbale
Chomveka kapena amber

Maganizo a kusankha rplc mbale

Kusinthasintha
Mukamvetsetsa nkhani ya galasi lagalasi, tidzakambirana za kusintha kwake. Kuphatikizidwa kwa kafukufukuyu kuyenera kuwunika posankha nthiti yofanana ndi mitundu ya vial.

1. Gwiritsani ntchito zimphona zofiirira za zitsanzo za zitsanzo zowoneka bwino. Zitsanzo za bulauni mu Mbale zitha kuwala bwino komanso kupewa kujambulidwa.

2. Masamba osinthira mwamphamvu amatengedwa mosavuta ndi galasi, akugwiritsa ntchito mitsuko yowirikiza.

3. Chifukwa cha trace zitsanzo zodziwika bwino, gwiritsani ntchito machubu amkati. Mutha kugwiritsanso ntchito machubu okhazikika, kapenaMbale zopambana kwambiri. Izi ndi zokhudzana ndi zitsanzo zachilendo zokhala ndi magwiridwe ang'onoang'ono.

4. Mukuwunika kwa ion, gwiritsani ntchito mipata yopangidwa ndi polypropylene (pp), osati miphika yagalasi.

Sankhani malinga ndi kuchuluka kwa zitsanzo

Kuti tipeze zitsanzo, tiyenera kugwiritsa ntchito mliri wa micro. Amapangitsa kuti mayesowo akwaniritse. Otsatirawa ndi Mbale zoyenera kutsatira zitsanzo zamalamulo osiyanasiyana.

1. Vesi la zitsanzo zosakwana 2mL
Mbale zimabwera m'mitundu yambiri.Pali Microsince Cials (15μl-800μl) ndi mtengo wobwezeretsa kwambiri (30μL-1.5ml). Palinso machubu amkati (100μl-400μl). Palinso 250μl polypropylene microspungu. Palinso mbale zamkaka zomwe zili ndi machubu omangidwa mkati (250μl-300μl).

2.2ml zitsanzo
Mbale zagalasi, Phula la Polypropyylene.

3.. Buku lachitsanzo likulu kuposa 2ml
Mipira ya 4ml zitsanzo, nsonga zam'matumbo, machubu, etc.

Kumvetsetsa mitundu yosiyanasiyana ya mipata yomwe imagwiritsidwa ntchito kwambiri - HPLC). Pitani mu gwero ili kuti mudziwe zambiri posankha zabwino zabwino za HPLC pazosowa zanu:Encyclopedia ya HPLC Vials

Sankhani malinga ndi njira yotseka

Mbale zoponderezedwa nthawi zambiri zimakhala zabwino kwa LC ndi LC \ / ma MS. Amakhala otsika komanso osintha. Poyerekeza ndi kapu ya crimp, njira iyi yotseka iyi siyikuvulaza manja ndipo sasowa zida zowonjezera. Screw-pa Zithunzi za Vials zimapezeka m'malo opangira mafuta omwe amapangidwira a Autosamurs ndi zolimba zopangidwira zosungira.

Chitsulo:Zida zapadera zimafunikira pakukamba.Amapanga chisindikizo chotetezeka nthawi yosungirako nthawi yayitali ikadalitsika kumanja. Mitengo ya ziphuphu-cap ndi yoyenera kwa gc ndi gc \ / ma MS. Popeza zikopa zachipongwe sizitha, zimapereka chitetezo chokwanira chakudya, zolosera, ndi mapulogalamu ena pomwe zitsanzo zachinyengo zimafunidwa. Ngati zosasunthika zimasanthula, zopaka ziphuphu zimalimbikitsidwa.

Swap Caps:Mphamvu zokopa za bayonet sizabwino monga njira zina ziwiri zopirira. The Bay Cap Stumps Vial SAPASANGALO, ndipo kapu ya bayonet ya bayonet ya TAP itha kuphimbidwa popanda zida zilizonse, zomwe ndizosavuta.

Gwiritsani ntchito yodikira ma petle kuti musindikize.Kukana kwake kuwonongeka ndi kusindikizidwa kumakhala kokhutiritsa. Ndi zotsika mtengo komanso zosavuta kugwiritsa ntchito, koma zimafunikira ntchito zochepa.

Kusankha chipewa choyenera cha Mtengo Wanu wa Chrometograph ndikofunikira kuti mutsimikizire zitsanzo ndikukwaniritsa zowunikira molondola. Nayi chitsogozo choti chikuthandizeni kusankha cholembera chanu cha chromatography: Momwe mungasankhire chipewa choyenera cha mkombe wanu wa chromatography?