Sep. 8, 2023
Chromatography ndi njira yowunikira yowunikira yowunikira yomwe asayansi amagwiritsa ntchito kupatukana ndikusanthula zosakaniza zovuta za mankhwala. Zotsatira zolondola ndi kiyi mu laboratories, ofufuza ma Institute, makonzedwe abwino kapena mabungwe abwino otsimikizika chimodzimodzi; Mmodzi mwa njira yobowolo ya cromamatography ikudzaza mtengo molondola poyesa kugwiritsa ntchito njirayi. Tikambirana za kufunika kwake mogwirizana ndi malangizo omwe angathandize kutsimikizira zotsatira zolondola mukamayesa zoyeserera zanu.
Chifukwa Chomwe Kudzazidwa Kwa Moyenera Kuli Kovuta Kwambiri
Zotsatira zolondola komanso zokulitsa chromatographict zimatengera mitundu yambiri, kuphatikiza zitsanzo, kusankha mtundu, chida cha ntchito, njira zakukonzekera, pakati pa ena. Gawo limodzi lofunika lomwe lingasinthe zotsatira zako kwambiri ndi momwe mumadzaza ndi kukonzekera mipata ya chrometographraphraphraphraphy. Ichi ndichifukwa chake nkhaniyi:
Kukhulupirika:Mbale zodzazidwa molakwika zingayambitse kuipitsidwa kapena kusinthasintha, kusintha kapangidwe kake ndikusintha zotsatira zanu.
Kubereka:Kusasinthika kuli pachimake cha chromatotography. Kukhazikitsa njira zatsopano zodzaza ndi vial iliyonse imakhala ndi zitsanzo zofananira kuti zikhale zotsatira zokhala ndi zotsatira zokhala ndi zotsatira zokhala ndi zotsatira zokhala ndi zotsatira zokhala ndi zotsatira zokhala zopambana.
Kukhazikika kwa Baseline:Kukhazikitsa kudzaza koyenera kumatha kukuthandizani kukhalabe ndi maziko okhazikika, ocheperako ndi kusokonezedwa ndi chromatogram.
Chitetezo cha Column:Kudzazidwa kwazikulu molondola kumatha kulepheretsa thovu kapena zitsanzo kuti zidziwike mu mawonekedwe anu a chromatography ndikuwononga mwanjira iliyonse.
Kuwongolera kwapadera poyang'ana bwino za chromatography
Tsopano kuti tikuthokoza chifukwa chodzazaMbale za chromatographyMoyenera ndizofunikira kwambiri, tiyeni tidutse gawo lililonse lofunikira kuti muchite bwino:
Gawo 1: Dziwani mtundu uti womwe mukufuna
Sankhani Mbale Zogwirizana ndi mitundu yanu ya chromatography ndi zitsanzo, kuphatikizapo zitsanzo zilizonse zowunikira kapena magwiridwe omwe Mbale zomveka bwino zingapindulitse. Komanso onetsetsani kuti ndi oyera komanso opanda nkhawa.
Mbale za chromatography zimabwera kumayiko osiyanasiyana, zida, ndi kutseka. Makulidwe odziwika kwambiri ndi 1.5 ml ndi 2 ml, ngakhale mavoliyumu akulu kapena ochepa amatha kugwiritsidwa ntchito kutengera zosowa zanu zapadera. Mbale zambiri amapangidwa kuchokera pagalasi la borosilthera, lomwe limapereka mphamvu ya mankhwala ndi kukhazikika kwa matenthedwe, kapena kuchokera ku polypropylene pazomwe zimafunikira pulasitiki.
Mukamasankha vial, lingalirani:
Kugwirizana ndi zitsanzo zanu:Onetsetsani kuti zinthu za vial sizimachita kapena kuipitsa chitsanzo chanu.
Zofunikira:Sankhani vial yomwe ingagwire samu yanu popanda kuyika ma spaillage kapena kusinthasintha.
Mtundu Wotsekemera:Kaya zipewa zomanga, zipilala, kapena zipewa, onetsetsani kuti kutsekedwa kumakhala kotetezeka komanso koyenera kwa njira yanu yowunikira.
Gawo 2: Lembani Mbale Zanu
Kulemba MbaleMolondola ndi wofunikira kuti musamale ndikupewa kusakanikirana pakuwunika. Gwiritsani ntchito zolembera za madzi kapena zilembo zolembedwa kuti zitsimikizire kuti zitsanzo zonse zichitike motere, monga dzina lake, gawo la kusanthula komanso kuchuluka kwa ntchito iliyonse.
Gawo 3: Konzani zokonzekera zitsanzo
Kukonzekera zitsanzo moyenera ndizofunikira kuti zitheke zolondola za chromatographic. Onetsetsani kuti mwakonzekeretsa m'njira yoti ndi yoyenera jakisoni wa chromagraphy - Izi zingaphatikizepo kanthu, kusefa kapena njira zina zogwiritsira ntchito njira yanu ya labu yokonzekera zitsanzo.
Mukufuna kudziwa momwe mungasankhire kachilombo ka HICP VS. SNAP VS. Conct Cap Vial? Onani nkhaniyi: CR. CRAIL VS. SNAP VS. Screct Cap Vial, momwe mungasankhire?
Gawo 4: Gwiritsani ntchito pipette yoyenera
Kupeza ndi kusunga bwino pipi yoyenera ndiyofunika kwambiri. Sankhani pipette ndi mitundu yofananira yomwe ikugwirizana ndi kuchuluka kwa fanizoli; A calibrate ndikutsimikizira kulondola kwake pafupipafupi kuti mutsimikizire zolondola; Gwiritsani ntchito malangizo oyenera a pipette kuti muchepetse kutayika kapena kuipitsidwa pakati pa zitsanzo.
Gawo 5: Kusamalira Mbale Mosamala
Kukhala ndi malo oyera ndikofunikira kupewa kuipitsidwa. Asanayambe ntchito, bungwe ndikuyeretsa malo anu ogwirira ntchito asanapereke magolovesi a laboratory kuti muchepetse chiopsezo cha kuipitsidwa. Ngati mwangozi mukukhudza mkati kapena mtunda wangozi mwangozi, siyani ndi kugwiritsa ntchito vial ina m'malo mopewa kuipitsidwa.
Gawo 6: Dzazani vial molondola
Kugwira mtengo wamaso kumathandizira kuti mukwaniritse mzere woyenerera ndipo kuti palibe mabowo omwe amapezeka mkati mwake.
Pakatopa zitsanzo, zimatulutsa pang'ono pang'onopang'ono kuti zizitha kuyendetsa madzi ndikupewa kutulutsa mafayilo osagwirizana.
Pewani kugwiritsa ntchito mphamvu zochuluka kwambiri popepuka chifukwa izi zingathetse zolakwika muzotsatira zanu. M'malo mwake, gwiritsani ntchito njira yofatsa komanso yokhazikika.
Gawo 7: tsekani vial yanu motetezeka
Caps kapena Septa adapangira mwachindunji chifukwa cha mankhwala anu a chromatography ayenera kusankhidwa. Ziphuphu zosindikizidwa bwino zimalepheretsa ndikusunga zitsanzo; owopsachisotiikhoza kuwononga Mbale kapena kusokoneza chisindikizo choyenera; Zotsatira zabwino Tsatirani ma abotale a labotale pa zisoti zosindikiza.
Gawo 8: Sakanizani ngati pakufunika kutero
Kuonetsetsa ngakhale kufalitsa popanda mpweya wa mpweya, ndikofunikira kuti zitsanzo zomwe zimakhala ndi zigawo zokhazikika kapena zigawo zomwe zingaphatikizidwe bwino mu zomwe zili mu zomwe zili mu zomwe zili mu zomwe zili mu zomwe zili mu zomwe zimapangitsa kuti zitheke (modekha), kusakaniza. Zovuta zitsimikizirenso kugawa popanda mizere ya mpweya yomwe imayambitsidwa mu kusakaniza.
Gawo 9: Sungani zidziwitso
Kusunga mbiri yolondola ndikofunikira kuti musinthe deta ndi kusanthula. Sungani buku lolondola la labotale kapena mbiri yamagetsi yomwe imaphatikizapo zambiri za zitsanzo zomwe zimatengedwa - zopatulikitsa zamtundu uliwonse kuchokera mu njira zilizonse zochokera ku zopatulikitsa - kuphatikizapo chilengedwe chilichonse chomwe chingapangitse zotsatira zake.
Gawo 10: Kusungidwa Koyenera
Kuteteza kukhulupirika kwa zitsanzo zanu, ikani zofunikira kuti zisungidwe. Zina zina zimafunikira kufinya pomwe zina ziyenera kusungidwa ndi kutentha kapena kutetezedwa ku kuwala. Funsani malangizo anu a labotale ndi machitidwe abwino okhudza kusungidwa kwa zitsanzo zabwino kwambiri.
Kudzaza Mbale Zoyenera Zabwino Kwambiri ndikofunikira kuti mukwaniritse zotsatira zolondola komanso zokuletsedwa mu zoyeserera zanu. Mwa kusamala mosamala zonse ndi protocols yomwe yakhazikitsidwa, mutha kuchepetsa zolakwa, onetsetsani kuti sangalalani ndi kuchirikiza miyezo yapamwamba kwambiri mu chromatographic kusanthula. Kumbukirani kuti kuwongolera mwachidule zitsanzo kumathandizira mwachindunji zotsatira zomwe zimapezeka mumasanthuyi.
Pezani chidziwitso chokwanira mu mafunso odziwika kwambiri a HPLC ndi nkhani yathu yodziwitsa. Tsegulani mayankho omwe mukufuna:50 mafunso ambiri omwe amafunsidwa kawiri pa HPLC Mbale
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