Ku China, zinthu zambiri zaulimi (zamankhwala ena, ma organic acid, ndi zina zambiri) zimafunikira kuzindikirika ndi chromatography yamadzi ndi gasi chromatography chaka chilichonse. Chifukwa cha kuchuluka kwa zitsanzo, ambirichromatographic zitsanzo mabotolo ayenera kutsukidwa pa ndondomeko kudziwika, amene osati kuwononga nthawi ndi kuchepetsa ntchito bwino, komanso nthawi zina kumayambitsa kupatuka kwa zotsatira zoyeserera chifukwa chosowa ukhondo chromatographic chitsanzo mabotolo pambuyo kuyeretsa.

Kuyeretsa mabotolo a chromatographic ndikofunikira kwambiri. Malingana ndi njira yotsuka ya chida cha galasi, sankhani njira yoyeretsera molingana ndi digiri ya kuipitsa, palibe njira yokhazikika. Chidule cha njira:

Yankho 1:

1. Thirani ndikuwumitsa madzi mumphikachromatographic vial.

2. Thirani zouma pambuyo pake pamene mowa umalowa mu 1.5mL vial mosavuta ndipo umasungunuka ndi zosungunulira zambiri za organic.

3. Thirani m'madzi ndikusamba ndi ultrasonic kawiri.

4. Thirani madzi mu botolo ndikuphika pa 110 ° C kwa maola 1 ~ 2. Osaphika kutentha kwambiri.
5. Kuziziritsa ndi kusunga

Chiwembu 2:

1. Tsukani madzi apampopi kangapo

2. Ikani mu beaker wodzazidwa ndi madzi oyera ndi ultrasound kwa mphindi 15

3. Sinthani madzi ndi ultrasound kwa mphindi khumi ndi zisanu

4. Zilowerereni mu beaker ndi kapena popanda ethanol

5. Chotsani zui ndikuwumitsa mpweya

Mukufuna kudziwa momwe mungapangire mbale zosiyanasiyana za HPLC?, Onani nkhaniyi:Crimp Vial vs Snap Vial vs. Screw Cap Vial, Mungasankhe Bwanji?

Yankho 3:

1. Zilowerere ndi methanol (chromatographic koyera) ndi kuyeretsa ndi akupanga kwa mphindi 20, ndiye kutsanulira methanol youma.

2. Lembani chromatographic chitsanzo botolo ndi madzi, nadzatsuka ndi akupanga kwa mphindi 20, ndi kukhetsa madzi.

3. Pambuyo kuyanikahplc mbale

Yankho 4:

1. Nthawi zambiri ndi ntchito yoyamba ya madzi aukhondo muzimutsuka kuyanika kenako ntchito sulphate dichromate potaziyamu odzola kumiza.

2. Njira yotsuka ya botolo la chromatographic ndi yofanana ndi yamadzimadzi. Choyamba, mowa wamankhwala umagwiritsidwa ntchito kuti zilowerere kwa maola oposa 4, ndiyeno akupanga kwa theka la ola

Yankho 5:

1. Ngati mtengo wake ndi wokwanira, gwiritsani ntchito zui yatsopano nthawi zonse

2. ngati mukufuna kugwiritsanso ntchito, njira yoyeretsera ndiyofunikanso kwambiri, choyamba ndi njira yothetsera oxidation (potaziyamu dichromate) zilowerere kwa maola 24, ndiye ndi madzi osungunuka pansi pa ultrasonic chikhalidwe katatu, pambuyo pa zui ndi kuyeretsa methanol, kuyanika kungagwiritsidwe ntchito.

3. MATS a botolo alowe m'malo ndi atsopano, makamaka posanthula zotsalira za mankhwala, apo ayi zotsatira zake zidzakhudzidwa.

Mukufuna kudziwa zambiri za momwe mungayeretsere mbale za chromatography, chonde onani nkhaniyi:Zothandiza! Njira 5 zotsuka mbale za chromatography

Zomwe zili pamwambazi ndi njira zisanu zoyeretsera mabotolo a chromatographic, tikuyembekeza kuti tikhoza kumvetsera kuyeretsa kwa hplc Mbale.

Zam'mbuyo:

Chromatograph imagwiritsa ntchito mbale zopanda kanthu ndi fyuluta ya syringe ya HPLC

Kenako:

Momwe mungayikitsire 20ml ya chromatograph vial

TAGS: hplc-autosampler-Mbale 2 ml-gc-botolo 2 ml - mbale zotsalira vinyo wosasa - 1.5 ml

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