Kuyenda Kwambiri Kumadzi Chromatography .

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Kupatukana

HPLC imagwiritsa ntchito hydrophobic kapena hydrophilic pafupipafupi kuti alekanitse. Itha kulekanitsa mankhwala osiyanasiyana olengedwa, polar ndi polar komanso osakhala ndi polar, pogwiritsa ntchito mitundu yosiyanasiyana ya magawo okhazikika ndi okhazikika ngati eyents. Kupatukana kumadalira kulumikizana kwa ofufuza kwa gawo la gawo lakale, lomwe limatha kutanthauzira kudzera mu gawo lokhazikika kuti muthe kusintha.

IC, makamaka, makamaka, imagwiritsa ntchito ion kusinthanitsa kumagwirizanitsa kwa ionic ndi polar. Gawo la masitima nthawi zambiri limapangidwa ndi ion-kusinthana ma rin, kulola kupatukana kogwira mtima kwa milatho ndi miziro. IC imagwiritsa ntchito maluso am'madzi, nthawi zambiri imakhala ndi madzi osungunuka omwe ali ndi mchere wosungunuka kapena ma asidi osungunuka, ndipo kupatukana kwambiri kumachitidwa mwanzeru popanda kuwongolera.

Mitundu Yosanthula

HPLC imasinthasintha ndipo imatha kuthana ndi mawonekedwe ophatikizika a zinthu zachilengedwe, zimapangitsa kuti ikhale yoyenera kugwiritsa ntchito mankhwalawa, chakudya, komanso kusanthula chilengedwe. Komabe, imakhala ndi malire potengera mtundu wa mitundu ya ionic, monga milatho yoyenera ndi masipoti, omwe nthawi zambiri samasungidwa bwino pazambiri za HPLC.

IC imaposa mtundu wa ionic ndi polar, ndikupangitsa kuti ikhale njira yomwe amakonda kuti apeze anyezi (ngati chloride ndi fluoride) ndi masikelo. Ndikofunika kwambiri m'minda monga kuwunika kwachilengedwe, chitetezo cha chakudya, ndi kusanthula kwa mankhwala, komwe ma ionic mankhwalawa afala kwambiri. IC imatha kudziwanso ma polar polar omwe ali ndi ionic pomwe gawo loyenera ndi gawo lam'manja limagwiritsidwa ntchito.

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Njira zodziwika

HPLC imakonda kugwiritsa ntchito zowunikira UV, zomwe zimafuna kusanthula kuti mutenge kuwala kwa UV. Izi zimabweretsa zovuta zofufuza zinthu zina, makamaka zomwe sizimayamwa kuwala kwa UV, monga ma ions ndi organic acids.

IC imagwiritsa ntchito kupezeka kwa anthu omwe amachititsa manyazi, komwe kumathandiza kwa mitundu ionic. Njira imatha kukwaniritsa njira zambiri pamaluso monga kupembenuza mankhwala, zomwe zimachepetsa nkhawa, kulola kuti mupeze magawo owunikira.

Mapulogalamu

HPLC imagwiritsidwa ntchito kwambiri pakuwunikira zovuta zophatikizika, kuphatikizapo zamankhwala osokoneza bongo, zowonjezera zakudya, komanso zitsanzo zachilengedwe. Kuthekera kwake pagululi kumapangitsa kuti ikhale chida champhamvu m'makampani osiyanasiyana.

IC imakhala yopindulitsa makamaka yofunsira kusanthula mitundu ya ionic, monga kuyesa kwamadzi, kusanthula chakudya, ndi kuyeserera kwa mankhwala opangira mankhwala. Nthawi zambiri imagwiritsidwa ntchito pokwaniritsa HPLC, kufalitsa kuthekera kwa ma labotor kuti muphatikizepo mitundu yochulukirapo ya ionic ndi polar.

Mwachidule. HplcNdioyenera kusiyanasiyana kwa mankhwala osiyanasiyana, pomwe ic ndi njira yosankhiratu kusanthula kwa mitundu ya ionic ndi polar.

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Ma tag: Hplcanayalysis VPLC-Mbale Mbale za HPLC-Galasi HPLC-Valis-mtengo Hplcvials HPLC-Vials-Ogulitsa HPLC-VialS-wopanga

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