Njira yoyendetsera madzi a chpomatography (HPLC) ndi njira yofunika yowunikira yomwe imagwiritsidwa ntchito kwambiri pakuwunika kwachilengedwe kuti mudziwe, kuchuluka, ndikuwunikira zoipitsa mu matricties monga madzi, nthaka, ndi mpweya. Monga nkhawa zowonongeka zachilengedwe zomwe zimayambitsidwa ndi ntchito zamafakitale, kutukuka kutukuka, maluso a ulimi amakula, HPLC yakhala yofunika kwambiri kuti iteteze zachilengedwe komanso kutsatira malamulo oyang'anira.
HPLC ndi njira yovuta yomwe imalekanitsa zinthu zomwe zosakanikirana malinga ndi zomwe amachita ndi masinthelo ndi mafoni. HPLC imakwaniritsa chiwonetsero chambiri komanso chidwi chogwiritsa ntchito kupanikizika kukakamiza madzi omwe ali ndi zida zodzazidwa ndi zida zokhazikika. Izi zimatha kuzindikira kuchuluka kwa zodetsa zomwe sizingalephereke komanso njira zosaganizira.
Ndikufuna kudziwa zambiri za kukonzekera kwa HPLC, chonde onani nkhaniyi:Mayankho a HPLC Kukonzekera kwa Zotsatira Zabwino
Akuluakulu a HPLC pakuwunika kwachilengedwe
1. Madzi oyeserera
Chimodzi mwazomwe zimagwiritsidwa ntchito kwambiri kwa HPLC pakuwunika kwachilengedwe ndi njira yabwino yoyeserera. Zovuta monga zitsulo zolemera, mankhwala ophera tizilombo, mankhwala osokoneza bongo, ndi endocrine akusokoneza Mankhwala amatha kuyambitsa ngozi zakuthanzi zaumunthu komanso zachilengedwe. HPLC imakupatsani:
Kuzindikira kwa Eldetant: HPLC imatha kuzindikira ndikugwiritsa ntchito zinthu zowopsa pakutsika kwambiri m'madzi. Mwachitsanzo, mankhwala ophera tizilombo omwe nthawi zambiri amapezeka kuti alimilira atha kupezeka.
Kuwunikira kwa Compliness: Mabungwe oyang'anira amafunikira kuwunikira matupi amadzi kuti awonetsetse kuti azitsatira mfundo zachitetezo. HPLC imapereka deta yodalirika yomwe imathandizira olamulira amafunikira njira zofunika kuteteza thanzi.
2. Kusanthula kwamphamvu
Dothi linanso lofunika kuwunikira zachilengedwe chifukwa imatha kudziunjikira zodetsa zamitundu yopangidwa ndi zodetsa za ulimi, zochitika za mafakitale, komanso kutaya zinyalala. HPLC imachita mbali yofunika kwambiri pankhani yotsatirayi:
Kuzindikiritsa zodetsa: HPLC imathandizira kuwunika chifukwa cha kuipitsidwa monga polycyclic orbonatic hydrocarbons (Pahs), ndi mankhwala ophera tizilombo.
Kubwezeretsanso njira zowunikira: m'mapulogalamu a biover
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HPLC imagwiritsidwanso ntchito posanthula zodetsa ndi kuwunikira mpweya wabwino. Mapulogalamuwa akuphatikiza:
Kuyesa kwa Ortic Cowiric Contround (Voc) Kuyesa: Vocs ndi mankhwala oopsa omwe amatulutsidwa kuchokera ku magawo osiyanasiyana, kuphatikizapo zojambula, ma sol sol, ndi mafakitale. HPLC imatha kupatukana ndi kuchuluka izi pazomwe zimapanga mizimu.
Kusanthula kwa tinthu: Njira iyi imathandizira kuzindikira tinthu tambiri tomwe timatha kukhudza thanzi kupuma komanso zimathandizira kuipitsa mpweya.
4. Kusanthula
Mayina a zinyalala ndizofunikira kutaya bwino komanso machitidwe obwezeretsanso. HPLC ikhoza kukuthandizani:
Pendani Mitsinje Yakuwonongeka: HPLC imathandizira kudziwa njira yoyenera yodziwiratu pozindikiritsa zigawo zowopsa mu zinyalala.
Onetsetsani kuti otetezeka: Kutsatira kwa ulamuliro kumafuna kuti zinyalala ziziwunikidwa bwino kuti zichepetse zoopsa zachilengedwe zomwe zimakhudzana ndi zolakwika.
Mukufuna kudziwa zambiri za mapulogalamu a Mbiri ya Chromatography, chonde onani nkhaniyi: Mapulogalamu 15 a Chromatography Vials m'minda yosiyanasiyana
Zabwino zogwiritsa ntchito HPLC pakuwunika kwachilengedwe
Pali zabwino zingapo zofunika kugwiritsa ntchito HPLC pakuwunika kwachilengedwe.
1. Chidwi chachikulu komanso molondola
HPLC imatha kuzindikira kuchuluka kwa zodetsa, ndikupangitsa kuti ikhale chida champhamvu chowunikira zoipitsa zomwe zimatha kukhala ndi zachilengedwe kapena zaumoyo. Kumvera kwambiri kumeneku kumalola kuti zisazindikiritse zoopsa zomwe zingakhale zovuta.
2. Kusiyanitsa
HPLC yakhala chida chosinthasintha kwa asayansi achilengedwe chifukwa imatha kugwiritsa ntchito kusanthula mitundu m'ma matricy (madzi, nthaka, mpweya, zinyalala). Mitundu Yosiyanasiyana ya Openda (UV-Wy-Fluorescence, Mass Spectometry, etc.) imatha kugwiritsidwa ntchito kutengera zomwe zikuwunika.
3. Borustness
Mphamvu ya HPLC Systems imawathandiza kuthana ndi matrices ovuta omwe amapezeka pazitsanzo zachilengedwe. Njira zoyenera zokonzekera, monga gawo lolimba.
Mukufuna kudziwa zambiri za mizere ya chromatography yamadzi, chonde onani nkhaniyi: Mitengo ya chromatography ya kusanthula kwamadzi
Makina oyendetsa bwino a cramatography imayima ngati chida chachikulu chowunikira mu chilengedwe chifukwa cha chidwi chosayerekezeka, kusinthasintha, komanso kukula. Ntchito zake zimakhala zamadzi zoyesedwa zamadzi, kusanthula kwadothi ndi nthaka, kuwunika kwa mpweya, ndi kuwononga zinyalala, aliyense amathandizira kwambiri kumvetsetsa kwa chilengedwe. Pamene kudziwitsa padziko lonse lapansi kumawonjezeka pakufunira zoyeserera zothandizira kuyesetsa kutchinjiriza kutchinjiriza thanzi la anthu komanso kusunga zachilengedwe m'mibadwo yamtsogolo.
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