Chidziwitso chosavuta cha chromatographic

Kusanthula kwa chromatographic ndi njira yolekanitsira ndi kusanthula kwa zosankha zosakanikirana. Zimagwiritsa ntchito kusiyana kwa malo owira, polarity ndi adsorrapt imodzi mwazinthu zonse zomwe zimapatukana ndi zojambulazo, ndipo zomwe zimadetsedwa zimayang'aniridwa ndi kusanthula kokwanira.

Chifukwa cha kuyeserera kwake kwakukulu, kuthamanga kwapenda mwachangu komanso kugwiritsa ntchito zochepa, njira zamankhwala, zokhala ndi thanzi, zotchinga zachilengedwe, zazachipatala. Chromatography imathetsa mavuto monga kuyendera asayansi, kafukufuku wa sayansi, kuwunika kwawonongeka kwa mafakitale opanga mafakitale ndi mafakitale m'magawo awa.

1) Chidziwitso Choyambira Chokhudza Mbale:
Zofunikira za Vial ndizolondola, zitha kupirira zovuta zina, kukhazikika kwabwino, ndipo palibe ADSORSESES pa chitsanzo. Woyenera ayenera kusankhidwa molingana ndi zofunikira za chida komanso mkhalidwe wa zitsanzo. Chipewa chosindikizira: Pofuna kupewa ganjenje kuti lisapangitse zigawo za zitsanzo, gasket ndi PTFF kapena Aluminium mkati mwanu. Zosankhidwa (kutentha) ndi momwe zitsanzozo zimadalira chitsanzo. Kusanthula kwa zinthu kumatha kuchitika ndi nsalu ya rubyl ndi mzere wa ma rabara a Sickene kuti asanthule. Ngati ndi kotheka, kusanthula kopanda kanthu kumatsimikizira kuti kusunthika kumangosungunuka sikusokoneza kusanthula.

2) za momwe mungasankhire vials
Choyamba, tiyenera kumvetsetsa kuti mbalezi ndizomwe zimawoneka bwino kwambiri.
Ngati mukufuna mtundu wa chilengedwe ndi acid-acid, mutha kusankha botolo lagalasi;
Ngati itha kugwiritsa ntchito mayunitsi yaying'ono kwambiri, chimbudzi chaching'ono chamkati chikufunika;
Silaneulization \ / kuwonongeka kumafunikira ngati amagwiritsidwa ntchito pazitsanzo ndi kusanthula komwe amalumikizidwa mosavuta kukhoma la botolo lagalasi;
Ngati igwiritsidwa ntchito pofuna kupenyerera, botolo la bulauni limafunikira;
Ngati imagwiritsidwa ntchito posankha mowa kapena kusungunuka kwamadzi, polypropylene amafunikira.

3) Kuchokera momwe mungayeretse botolo lachitsanzo
M'malo mwake, tikamachita madzimadzi a chromatography.Mbale za HPLCndi imodzi yotsika mtengo, koma mtengo wake ndi wokwera kwambiri, kotero tsopano tifunika kugwiritsa ntchito vials yobwerezedwa, tiyenera kukhala oyera. Titha kuyesa kugwiritsa ntchito ma reagent oyeretsa. Ethanol itha kugwiritsidwa ntchito kuyeretsa. Ngati imagwiritsidwa ntchito mwachangu, imatha kutsukidwa ndi ethanol kenako ndikuliwala ndi chowuma tsitsi.