Kusanthula kwa HPLC kwachitika patsogolo kwambiri zaka zaposachedwa chifukwa cha matekinoloje anzeru. Kupita patsogolo kotereku ndiMa incro Valu; Kugwiritsa ntchito kwawo kwatsimikizira kusintha kwa ofufuza ndi akatswiri pokonzekera ndi jakisoni. Tidzayang'ana mumtima mozama mu dziko losangalatsali, kukambirana za kufunika kwake, njira zapadera, mapulogalamu, ndi zabwino, ndi zabwino mwa kusanthula kwa HPLC
Kuyambitsa kwa Micro Vial
Pamtima pa kuwunika kwa HPLC kumakhala kolondola komanso kotsimikizika kwa zitsanzo kulowa mu makina owunikira. Gawo lovuta limeneli limachita mbali yofunika kwambiri popeza zotsatira zodalirika komanso zobereka.Ma incro Valu, nthawi zambiri imagwiritsidwa ntchito molumikizana ndi vyoni, apambana gawo ili la kusanthula kwa HPLC popereka zabwino zomwe zimapangitsa kuti zikhale zopepuka, zichepetse kumwalira kwake, ndikumatha kukonzekera.
Kukulitsa kulondola ndi vyt mu hplc

Ikani vial hplc ikutanthauza mchitidwe wogwiritsa ntchito mipata yomwe ili ndi zikwama zamicro ya kusanthula kwachitsanzo, ndikuwonetsa kuwongolera kwakukulu komanso kulondola kuwunika kwa data. Akatswiri amatha kugwiritsa ntchitoIkani MbaleKuti mukwaniritse mphamvu zazikulu pa nkhani yoyambitsa, sinthani ngozi yodetsedwa, sinthani mawonekedwe onse, mafashoni ochulukirapo, ndikukweza mphamvu yolekanitsa, ndikuwonjezera ma chromatographic.
Mtundu Wosiyanasiyana

Mtundu wa kulowetsa	Kaonekeswe	Kukula	Malaya	Kukula	Kulemera	Cakusita	Mawonekedwe
Kuyika kwa micro	Adapangidwira kuchuluka kwa zimbudzi	150 μl - 300 μl	Magalasi a Borosil	350 μL - 500 μl	0,5 g - 1 g	Makonda apulasitiki	Kuchepetsedwa kutayika kwachitsanzo, kumagwirizana ndi autosamu
Ikani	Zoyenera Kusanthula Kwambiri	250 μl - 500 μl	Polypropylene	500 μl - 1 ml	0.8 g - 1.2 g	Mapaketi Ochuluka	Mosiyanasiyana komanso wodalirika, wogwirizana ndi HPLC Systems
GC ikani	Zopindika kutentha kwambiri komanso kukakamizidwa	100 μl - 300 μl	Adasankhidwa silica	300 μl - 500 μl	0.3 g - 0.8 g	Mbale zamphongo	Adapangidwira kusanthula kwa GC, CET ndi kugwiritsidwa ntchito molakwika
Autosampr	Yogwirizana ndi autosamu	150 μl - 300 μl	Ptche	250 μl - 500 μl	0.6 g - 1 g	Mbale zapulasitiki	Zimatsimikizira zitsanzo zokha, zikugwirizana ndi autosamu
Kuyika	Kapangidwe kopangidwa ndi kuphatikizika kwa kusakaniza	200 μl - 400 μl	Polypropylene	400 μl - 800 μl	0.7 g - 1.1 g	Mapaketi Ochuluka	Imathandizira homogenization ndikusakaniza zitsanzo
Ikani	Zinthu zisanachitike septa ya kuboola kosavuta	250 μl - 500 μl	Silicon \ / ptfe	500 μl - 1 ml	0.9 g - 1.3 g	Mbale zapulasitiki	Scaliteifies Selelleies Kulowa kapena kumachepetsa
Kalasi yapadera	Galasi yapamwamba kwambiri ya zitsanzo za zitsanzo	200 μl - 400 μl	Galasi lotsika kwambiri	400 μl - 800 μl	0.6 g - 1 g	Mbale zamphongo	Amachepetsa zitsanzo adsorption ndi kukulitsa kuchira
Chitsulo	Ntchito yomanga zitsulo kuti igwiritsidwe ntchito	100 μl - 300 μl	Chitsulo chosapanga dzimbiri	300 μl - 500 μl	0.4 g - 0.9 g	Mbale zamphongo	Kugonjetsedwa ndi Kupsinjika kwa Mankhwala ndi Makina

Zojambula zowunikira ndi Autosambirr ikani Mbale

Kuphatikiza kwa makina odzicepetsa, monga Autosamurs, zasinthasintha kwa ntchito ya HPLC.Autosamppler Ikani Mbale, zopangidwa kuti zigwirizane ndi zoyika za micro, kusewera gawo lofunikira pakuwunikira makope ndikulimbikitsa zokolola mu labotale. Mbale izi zimapangidwa kuti zizigwirizana ndi autosamu, zomwe zimapangitsa kuti jakisoni azikhala ndi jakisoni, wofooketsa ntchito, ndikuchepetsa kuthekera kwa cholakwa cha munthu. Kuphatikiza kwa autosamppler kuyika Mbale ndi Micro Valung Sceriction Njira Yothandizira, Yothandizira Kuyesa Kugwiritsa Ntchito Mavidiyo Akuluakulu ndikukwaniritsa.
Kuyang'ana kusiyanasiyana kwa GC kuyika Mbale

Ngakhale HPLC ndi njira yothandiza kwambiri yowunikira, gal chromatography (GC) imagwiranso ntchito yake yomwe ili mu minda yosiyanasiyana.GC ikani Mbalezopangidwira mwachindunji za mapulogalamu achinsinsi a chromatographic amapereka mgwirizano wapadera komanso magwiridwe apadera; Akaphatikizidwa ndi mivi yabwino yomwe amawonetsa bwino, zomwe zikutsogolera kusintha zida zosinthika zimapangitsa kuti zikhale ndi zitsanzo zowonjezera \ / kugwirira ntchito mphamvu yolimbikitsidwa \ / kugwirira ntchito mphamvu yolimbikitsidwa
Micro ikani Mbale: Kuthandizira kuwongolera muyezo wochepera

Micro ikani Mbalendi zida zopindulitsa zomwe ofufuza amagwira ntchito ndi mayunitsi ochepa. Mphamvu yaying'ono ya mbalezi, yophatikizidwa ndi micro vial, imalola kusanthula kwa mphindi zazifupi, kuchepetsa kufunika kwa mafilimu akuluakulu owunikira pomwe akusangalala. Kapangidwe koyenera ndi kusindikizidwa kwa micro kuyika Mbale zoletsa kutayika kwa zitsanzo, kuzipitsidwa, komanso kuipitsidwa, kusungidwa kukhulupirika kwa kafukufuku konse. Kaya mu kafukufuku wopanga mankhwala, kusanthula kwachilengedwe, kapena mau asayansi ena, micro ikani Mbale Mbale zimapatsa mphamvu ofufuza kuti apeze zotsatira zolondola komanso zolondola.

HPLC ikani kusinthasintha kwabwino komanso kuchita bwino mu chiwerengero cha HPLC

5 Ubwino wa Kuyika kwa HPLC: Kusambitsa kuthekera

Kuyika kwa HPLC, kuphatikiza zingwe za micro, perekani zabwino zosiyanasiyana zomwe zimalimbikitsa gawo la chemistry. Tiyeni tiwone phindu lofunikira pakugwiritsa ntchito ziwonetserozi mu HPLC kusanthula kwa HPLC:

1. Kuchulukitsa komanso kulondola:

Kapangidwe kakang'ono ndi miyeso ya zingwe za HPLC, kuphatikiza ndi zoyikamo zowonjezera, onetsetsani kuti zotsatira zake. Chikhalidwe chokwanira cha Micro Valung Stople Kuchepetsa Kutayika Kwachitsanzo Muka jakisoni, kulola kuti voliyumu yonseyi ifika pachiwonetsero chazowunikira. Izi zimalepheretsa zolakwika zomwe zimachitika chifukwa cha zitsanzo za zitsanzo ndikuwonetsetsa kuchuluka komwe kumachitika mosavuta. Kugwiritsa ntchito zingwe za HPLC kumapangitsa chidwi cha njira zodziwika, zomwe zimapangitsa kusanthula kwa osankhidwa otsika kwambiri molondola kwambiri.

2. Makamaka kukhulupirika:

Chimodzi mwazovuta zomwe zikuwunika mu HPLC zikuwunikira ndikusungabe umphumphu wa chimbudzi chonse.Kuyika kwa HPLCSewerani gawo lofunikira pakusunga umphumphu pochepetsa kulumikizana pakati pa zitsanzo ndi zapamwamba. Izi zimalepheretsa kusanthula, kuwonongeka, komanso kuipitsidwa, kuwonetsetsa kuti zotsatira zabwino komanso zodalirika. Zipangizo zogwiritsidwa ntchito popanga zingwe za HPLC, monga galasi kapena poyintrars apadera, kupereka malo abwino osungitsa zitsanzo.

3. Kugwirizana ndi autosamussers:

Autosamurs akhala zida zofunikira mu labotale yamakono, kupangitsa kusanthula kwapamwamba kwambiri. Kuyika kwa HPLC kwayamba kuyenera kukhala kosawoneka bwino mu njira zamagetsi zoyambira zosasangalatsa komanso jakisoni. Kuphatikizika kwawo komwe kumathandizira pakuyenda bwino, kumachepetsa ndalama zogwirira ntchito, ndikuwonjezera mphamvu yonse; Openda amatha kufalitsa zingwe zamitundu yosiyanasiyana pamalingaliro osiyanasiyana a HPLC pakusinthasintha kwakukulu ndikugwiritsa ntchito mosavuta.

4. Zotsatira Zosachedwa:

Kunyamula, kapena kuipitsidwa ndi makanema m'mbuyomu, kumatha kulepheretsa molondola komanso kudalirika kwa zotsatira za HPLC. Ma incro Valungs amatenga mbali yofunika kwambiri pochepetsa zotsatira za kunyamula zotsatira popereka chotchinga pakati pa zitsanzo. Mphamvu yotsimikizika ya HPLC ikuletsa kuipitsidwa ndi kuipitsidwa ndi kusanthula, ndikuwonetsetsa zolondola ngakhale zikugwira ntchito yolondola ngakhale mutagwira ntchito ndi matricts zovuta kapena kafukufuku.

5. Kusiyanitsa ndi kusinthasintha:

Kuyika kwa HPLC kumabwera kumayiko osiyanasiyana, kapangidwe kake, ndi zida zothandizira kusamalira zinthu zosiyanasiyana. Kutengera ndi kuchuluka kwa zitsanzo, zofuna zogwirizana, ndi zolinga zowunikira, ofufuza amatha kusankha kuchokera pamitundu ya micro. Ma invice ena atha kukhala ndi mawonekedwe apadera, monga mawonekedwe owoneka bwino a kusakaniza kapena ma flits owonjezera kufesa. Kusintha kumeneku kumalola kusanthula kugwirizanitsa makina awo a HPLC pa mapulogalamu apadera, kuyesetsa kuchitapo kanthu komanso kupeza zotsatira zabwino kwambiri.

HPLC ikani kusinthasintha kwabwino komanso kuchita bwino mu chiwerengero cha HPLC
Kusankha kuyika kumanja kwa HPLC
Kusankha zoyika zoyenera HPLC pakusanthula kwina ndikofunikira kuti muwonetsetse bwino magwiridwe antchito komanso zotsatira zabwino. Nawa zinthu zina zofunika kuziganizira mukamasankha zikwangwani za HPLC:

1. Kugwirizana kwazinthu:

Zinthu zaHPLC ikaniiyenera kukhala yogwirizana ndi matrix ndi solvent system. Zida zodziwika bwino zimaphatikizapo galasi, lomwe limapereka chisamaliro chabwino, komanso ma pointmers apadera, omwe amapereka kukana kwa mgwirizano wapadera. Ganizirani mtundu wa zitsanzo ndi zowunikira kuti musankhe zinthu zoyenera kwambiri.

2. Ikani mapangidwe ndi miyeso:

Samalani ndi kapangidwe ka HPLC Ikani, kuphatikiza kutalika, maincheni, ndi mawonekedwe. Onetsetsani kuti kuyikako kumatha kukhala kosalekeza mu vial ndipo kumagwirizana ndi makina a Autosambir. Kukula koyenera ndi kuyimitsidwa ndikofunikira kuti musindikize komanso mawu oyamba.

3..

Ganizirani magawo omwe angafune kuti musanthule. Zolemba za HPLC zimapezeka mu mphamvu zosiyanasiyana, kuyambira 0,1 mpaka 1 ml. Sankhani kuyika ndi kuthekera koyenera kuti igwirizane ndi zitsanzo za zitsanzo popanda kunyalanyaza chidwi kapena kulondola.

4.. Mawonekedwe apadera:

Kuyika kwina kwa HPLC kumapereka mawonekedwe apadera kuti athe kuthana ndi zovuta zina. Mwachitsanzo, zoyika ndi zopanga zamakono zimathandizira kusakanikirana bwino, kuonetsetsa kuti homogeneity mu chitsanzo. Maina odzaza masika amapereka katundu wokhazikika, kuchepetsa chiopsezo cha kutaya. Fotokozani zofunikira zanu mwachidule ndikuwona ngati mawonekedwe aliwonse apadera omwe amaperekedwa ndi zigawo zina za HPLC angapindule ndi kusanthula kwanu.

5. Kudalirika:

Kusankha wogulitsa wodalirika kwa HPLC kuyika kwa HPLC ndi kiyi kuti musunge bwino malonda ndi kusasinthika. Yang'anani othandizira ndi zotsimikizika zotsimikiziridwa zotsimikizika zodalirika; Onani zinthu monga kupanga miyezo, kuvomerezedwa ndi mankhwala ndi ndemanga kasitomala popanga chisankho chidziwitso.

Ntchito za HPLC

Kuyika kwa HPLCPezani mapulogalamu ambiri asayansi osiyanasiyana ndi mafakitale. Nayi madera ena omwe amawoneka omwe akuyika HPLC amagwiritsidwa ntchito kawirikawiri:

Kusanthula kwa mankhwala:

Mu kafukufuku wofufuza mankhwala komanso kuwongolera kwapadera, HPLC ndi chida chofunikira pakuwunika mankhwala. Kuyika kwa HPLC kumathandizira molondola komanso molondola zokhala ndi mankhwala (apis) ndi zodetsa, onetsetsani kuti amatsatira njira zovomerezeka. Kugwiritsa ntchito ma vial vial kumachepetsa zofunikira zitsanzo, ndikuwapangitsa kukhala oyenera kupenda kuchuluka kwa mtengo wapatali kapena kochepa.

Kusanthula Zachilengedwe:

Kuyang'anira zachilengedwe kumaphatikizapo kusanthula kwa zoipitsa zosiyanasiyana, monga mankhwala ophera tizilombo, herbicides, ndi zodetsedwa m'madzi, nthaka, nthaka, ndi misa zitsanzo. Makina a HPLC amathandizira kusanthula matoma ovutawa posintha zitsanzo zothandizira, kuchepetsa zingwe, komanso kulimbikitsa chidwi. Kufanana kwaKuyika kwa HPLCNdi ma steji osiyanasiyana komanso njira zodzidziwitsa zimawapangitsa kukhala ndi mosiyanasiyana pakuwunikira chilengedwe.

Chakudya ndi Chakunja:

Kusanthula kwa chakudya ndi chakumwa cha HPLC kumagwiritsidwa ntchito kwambiri kuti atsimikizire kuti chitetezo chambiri, chabwino, komanso kutsatira malamulo oyang'anira. Kuyika kwa HPLC kumasewera mbali yofunika kwambiri pozindikiritsa odetsa monga mycotoxins, zotsalira za mankhwala, komanso zowonjezera - osatchulapo zakudya zochepa kapena zakudya zotsika mtengo.

Kusanthula kwamankhwala ndi kutchula

M'malonda achipatala komanso kuwonetseratu, HPLC imagwiritsidwa ntchito pakusanthula mitundu yosiyanasiyana yazomwe amagwiritsa ntchito mankhwala, mankhwala osokoneza bongo, komanso zinthu zoopsa pazitsanzo zachilengedwe. Makina a Micro Vial amapereka zitsanzo molondola komanso zodalirika, kulola kuwerengera molondola kwa matricts mu matricts. Kugwirizana kwawo ndi autosamplers kumapangitsa kuti pakhalepo ndi kuchita bwino kwa chipatala.

Mapeto

Ma incro Valutasintha kusanthula kwa HPLC mwa kukonza molondola, kulondola, komanso kuchita bwino. Kutha kwawo kuchepetsedwa kutayika kwachitsanzo pokhalabe ndi mtima wosagawanika, makilofu am'madzi, ndipo mavuto otsalira amawapangitsa zida zapamwamba zamakono. Kugwiritsa ntchito kwawo kumatsimikizira zotsatira zodalirika ngakhale zitagwiritsidwa ntchito m'njira zofufuzira, kuyesedwa kwa chilengedwe, njira zamankhwala, zomwe zimagwiritsidwa ntchito molingana ndi bwino m'tsogolo.

Zomwe Mungamvere

Dziwani nokha za kusinthika kwa zipolopolo zamicro pamene amatenga mbali yofunika kwambiri pakuwunika kwa HPLC, kuchepetsa zoopsa za HPLC Malingaliro odalirika asayansi.

Lumikizanani ndi ine tsopano!

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Takonzeka kumva kuchokera kwa inu ndikukupatsirani ntchito zapadera.

M'mbuyomu:

Mapulogalamu a Mzere wa chromatography mu chakudya ndi chakumwa chakumwa

Ena:

Zifukwa zitatu zapamwamba zomwe zimapangidwira miyala chromatography ndiyabwino kuposa Mbale zapulasi

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