Chifukwa chiyani GC-MS imagwiritsidwa ntchito poyesa mankhwala?

Mafuta a chromatography - misa sportmetry (GC-MS) amadziwika kuti ndi njira yowunikira yoyeserera yoyeserera mankhwala, makamaka m'minda yazachipatala komanso yowonetsera. Kutha kwake kupereka zotsatira zolondola, zodalirika, komanso zotsatira zabwino zimapangitsa njira yosankha kuti adziwe ndi zochulukitsa mankhwala ndi metabolites awo zitsanzo zachilengedwe. Blog iyi ikuwona zifukwa zogwiritsira ntchito GC-MS pakuyesa mankhwala, kuyang'ana pa zabwino zake, njira, ndi ntchito.

Mukufuna kudziwa zambiri za kusiyana pakati pa LC-MS ndi GC-MS, chonde onani nkhaniyi: Kodi pali kusiyana kotani pakati pa LC-MS ndi GC-MS?

Njira ya GC-MS

GC-MS kuphatikiza njira ziwiri zowunikira: gasi chromatography (GC) ndi Sportrometry (MS).

Ma chromatography: Mu gawo loyambirira ili, sampuliyo imaphatikizika ndikugawika mu zinthu zake pogwiritsa ntchito gawo la capillary. Kupatukana kumakhazikika pa owira ndi kuphika korona ndi polarity wa mankhwala, kulola kuti kusiyanasiyana kwamavuto ovuta.

Misa Spectmetry: Zigawozo zitalekanitsidwa, zimayambitsidwa mu misa misa. Pano, ali ndi mainyo ndipo ma iyo amasanthula chifukwa chotengera kuchuluka kwawo kwapadera. Izi zimabweretsa misa yapadera pagawo lililonse, ndikupereka chidziwitso chokwanira komanso chokwanira.

Njira ziwiri izi zimalola kuzindikiritsa kolondola kwa zinthu zomwe zili mu chitsanzo, kupanga GC-MS makamaka kuti muyezedwe mankhwala.

Ubwino wa GC-MS poyesa mankhwala

1. Kuzindikira kwambiri komanso mwachindunji

Chimodzi mwazifukwa zazikulu zogwiritsira ntchito GC-MS pakuyesa mankhwala ndi chidwi chake:

Kuzindikira kwambiri: Uwu ndi wovuta kwambiri pamakina azachipatala, pomwe odwala angatenge mankhwala ochepa kapena metabolite.

Chidziwitso Chachindunji: Magawo Ambiri amapereka chidziwitso mwatsatanetsatane za kapangidwe kake ka chizindikiritso cha pawiri, zomwezimitsa ngakhale zinthu zomwe zofanana. Izi zimathandizira kuchepetsa zabwino zomwe zingachitike ndi njira zina zowunikira.

2. Kuyang'ana bwino

GC-MS imatha kujambula zinthu zingapo:

Kuyeserera kwamankhwala angapo: ukadaulo ungathe kupenda mankhwala angapo ndi ma metabolites m'njira imodzi. Kuthekera kokwanira ndikofunikira mu matenda am'mimba, pomwe odwala angadziwike ndi zinthu zosiyanasiyana.

Kusintha kwa zinthu zatsopano: Pamene mankhwala atsopano amabwera kumsika, zinthu izi zimatha kuphatikizidwa mu protocol ya GC-MS posintha magawo kapena datase ya library yomwe imagwiritsidwa ntchito pozindikiritsa.


Kodi mukudziwa kusiyana pakati pa VPLC Vials ndi Mbale za GC? Onani nkhaniyi:Kodi pali kusiyana kotani pakati pa mbale ya HPLC ndi Mbale za GC?

3. Kuyesa kotsimikizika

Ngakhale mayeso oyambira kuwunika, monga Amonussassis, angawonetse kukhalapo kwa mankhwala, nthawi zambiri sangatsimikizire kuti:

Kusanthula Kotsimikizika: GC-MS ikhoza kugwiritsidwa ntchito ngati mayeso otsimikizira pambuyo poyambira. Zotsatira zabwino kuchokera ku iMunzissiy imatha kutsimikiziridwa ndi kusanthula kwa GC-MS, kupereka umboni wofunikira wogwirizana chifukwa cha zosankha zalamulo kapena zamankhwala.

Kutsatira Kwalamulo: Zojambula Zamalamulo

Mapulogalamu a GC-MS pakuyesa mankhwala

1.

Muzachipatala a Laboratories, GC-MS nthawi zambiri amagwiritsidwa ntchito posanthula zitsanzo za mkodzo pa mankhwala osokoneza bongo a nkhanza:

Milandu yamankhwala osokoneza bongo ndi poizoni: Imagwira ntchito yofunika kwambiri pakuwunika kwa odwala omwe amasinthidwa chifukwa cha poyizoni kapena poyizoni. Mwa kuzindikira zinthu zomwe zili mu mkodzo, zipatala zimatha kupanga zisankho zizindikilo.

Kuwunikira kugwiritsa ntchito mankhwala osokoneza bongo: GC-MS imagwiritsidwanso ntchito kuwunikiranso zomwe anthu amagwiritsa ntchito, zikuwonetsetsa kuti odwala akumwa mankhwalawo ndipo sakugwiritsa ntchito kapena kuwazunza.

2. Kafukufuku wofufuza

GC-MS imagwiritsidwa ntchito kwambiri kuphunzira mankhwala osokoneza bongo ndi pharmacokinetics:

Kusanthula Metabolite: Ofufuza amagwiritsa ntchito GC-MS kuti afufuze metaboli opangidwa pambuyo popangira mankhwala, omwe amathandizira kumvetsetsa momwe mankhwala amapangidwira m'thupi.

Kukula kwa Njira Zatsopano Zowunikira: Kusintha kwa GC-MS kumalola ofufuza kuti apange njira zatsopano zomwe zimapangidwira mankhwala kapena matrics, potengera luso la kuyezetsa mankhwala.

Mapeto

Mafuta a chromatography - misa sportmetry (GC-MS) wakhala ukadaulo wagolide wa kuyesa mankhwala chifukwa cha chidwi chake chachikulu, mwachindunji, komanso kuwunika kwathunthu. Kutha kwake kupereka zotsatira za masitepe kumapangitsa kuti zikhale zofunikira kwambiri kuchipatala komanso kusanthula komweko. Mankhwala atsopano akamapitirirabe ndikukula, kusintha kwa GC-MS kumatsimikizira kuti imakhala patsogolo pa mankhwala oyeserera.

Pogwiritsa ntchito ukadaulo wa GC-MS MS-MS


Kuti mumve zambiri pa Autosambir Mbale za Chromatography, fotokozerani nkhaniyi: 2 ml Autosambir Mbale za Chrometography