Chromatography, Njira Yachinangy mu Chenchical Chemistry, imathandizira kulekanitsa ndi kuchuluka kwa zigawo zikuluzikulu mu zosakaniza zovuta. Ngakhale kuti anali wofala, a chromatographic amatengeka ndi kusokonekera komwe kumachitika, komwe kungasokoneze kulondola komanso kolondola. Gwero limodzi lofunika kupezeka kuchokera ku mipanda ya chromatography, yomwe imatha kuyambitsa zodetsa ndi zojambulajambula kutrix. Munkhaniyi, timasamala za kusokonekera kochokera kuMbale za chromatography, Kufufuza zomwe zimayambitsa komanso njira zopangira zinthu zosokoneza.

Kuzindikira Kuyambira Kuyambira Kuyambira

Zotsalira zodetsa nkhawa

Mitengo ya chromatography imatha kusunga zodetsa zopanga pogwiritsa ntchito njira kapena kugwiritsa ntchito kale. Izi zimasokoneza zinthu zingapo, kuphatikiza mafuta, mafuta omwe amatulutsa nkhungu, kapena zowonjezera polymer. Pamene awa atayipitsa iwo mu zitsanzo, amayambitsa phokoso lam'munsi ndikusokoneza chizindikiritso ndi kuchuluka kwa zomwe mukufuna. Kukhalapo kwa omwe ali ndi vutoli kumatha kubweretsa nsonga zonama, kuchepa kwa zikwangwani zakunja, komanso kunyengerera chromatographic.

Mukufuna kudziwa zambiri za kugwiritsa ntchito Mipata ya chromatography, chonde onani nkhaniyi:Kuyang'ana njira zowunikira zachilengedwe zamiyendo ya chromatography (6 makamaka pogwiritsa ntchito)

Zowonjezera ndi zopepuka

Zida zina za vial, makamaka plastics, zimakhala ndi mawonekedwe oti amasule mankhwala omwe apezeka kuti adzipha kapena zitsanzo. Izi zimatha kuchokera ku matrix kapena zowonjezera zomwe zimagwiritsidwa ntchito mu nsanje zake. Atalowa m'chitsanzo, zinthu izi zitha kuipitsa dongosolo lowunikira, zomwe zimapangitsa kuti zisachitike ndi zomwe zimasanthula. Kukhalapo kwa zowonjezera kumabweretsa zovuta kwambiri pakusanthula kwambiri, makamaka m'mafakitale opangira mankhwala ndi zakudya zomwe zimadziwika.

Pamtunda adsorption

Chromatography vialMalo amatha kuwonetsa katundu wa Adsortive, pomwe amadzisankhira kapena zitsanzo zikuluzikulu zimagwirizana ndi makoma a vial. Izi zimatchulidwa makamaka m'magalasi agalasi, pomwe magulu a Silanol pamtunda amatha kulumikizana ndi polar kapena zolipiritsa. Pamtunda Adsissaption imabweretsa kuwonongeka kwa zitsanzo, kenako yolemerera, ndipo nthawi yokhazikika, potengera kutanthauzira kwa cromatographic ndikuchepetsa chidwi chachikulu.

Njira Zosokonekera

Kusintha kwa Vial

Kusankha Mbale Zopangidwa ndi zida zomwe zimakhala zosavuta kuderali ndikofunikira kuti muchepetse zinthu zakale. Mimba yamagalasi, yodziwika bwino chifukwa cha kufooka kwawo ndi mbiri yotsika mtengo, ndi chisankho chokonda kwambiri pamapulogalamu ambiri. Kapenanso, mipata yokutidwa ndi ma olima okwera, monga polytetrafluoro sollene (ptfe) kapena silika yowonjezera, potengera ma adsorthaphic magwiridwe antchito.

Chofunika Kwambiri Kusankha pakati pa Mbale Zamlamwi, Mbale Zosavuta, ndi Mbale Wapamwamba? Onani nkhani iyi kuti muzindikire posankha njira yoyenera yofunikira. Onani tsopano !:CR. CRAIL VS. SNAP VS. Screct Cap Vial, momwe mungasankhire?

Zowongolera

Isanachitike zitsanzo, Mbale ziyenera kukhala zowongolera kwambiri kuti muchepetse zodetsa zotsalira ndikuchepetsa kusokonekera. Njirayi imafunikiranso kulira ndi ma solthal oyenerera, monga methanol kapena madzi, kutsatiridwa ndi kuyanika pansi pa mikhalidwe. Mwa kuchotsa magwero osokoneza, chisanachitike onetsetsani kukhazikika kwa zizindikiro zoyambira.

Kuwongolera kwapadera

Kukhazikitsa njira zowongolera zowongolera ndikofunikira pakuwonetsetsa kukhulupirika kwa chromagraphy ndikuchepetsa kusokonekera. Laboratories ayenera kukhala ndi masiyidwe aukhondo owoneka bwino, poyang'ana zoperewera, tinthu tating'onoting'ono, kapena zotsalira. Mbale ziyenera kusungidwa m'malo oyera ndi olamulidwa kuti mupewe kuipitsidwa pakati pa kugwiritsa ntchito, ndi Mbale zilizonse zomwe zanyalanyazidwa kuti zitha kupewa zolakwika.

Kusamalira zitsanzo

Kuchita maluso oyenera ogwiritsira ntchito zitsanzo kumathandizira kupewa zokhudzana ndi chromatography. Openda ayenera kusamala kuti achepetse zitsanzo zapamwamba, kupewa mbale zamfuti ndikuwonetsetsa kuti kusindikiza koyenera kuti mupewe kusinthasintha kapena kuipitsidwa. Kuphatikiza apo, kugwiritsa ntchito zitsanzo kapena kuphatikizika kumatha kuchotsa tinthu tating'onoting'ono komanso kuchepetsa chiopsezo cha zitsanzo za kunyamula, potero kumalimbikitsa kulondola komanso kuwongolera zotsatira za chromatographic.

Mukuyang'ana zakuya ku HPLC zitsanzo kukonzekera? Pitani munkhani yodziwitsa iyi kuzindikiritsa ndi maupangiri ofunikira kuti mukonzekere malonda anu a chromatot. DZIWANI ZAMBIRI tsopano!:Mayankho a HPLC Kukonzekera kwa Zotsatira Zabwino

Njira Kutsindika

Kukonza magawo a chromatographic kumatenga gawo lofunikira pakuchepetsa chidwi cham'mbuyo ndikuwunikira. Openda amayenera kuwunika mwadongosolo njira, kuphatikiza gawo lam'manja, umagwiriramo wofanizira, ndi magawo okwanira, ndikugwiritsa ntchito njira zopatukana ndi zikwawa. Mikhalidwe yabwino kwambiri imatha kuchepetsa zotsatira za phokoso lakumbuyo, sinthani chipongwe, ndikuwonjezera kudalirika kwa miyeso yochulukirapo.

Mapeto

Kusokoneza komwe kunachokera kuchokeraMbale za chromatographyimabweretsa vuto lowopsa kuti mukwaniritse zotsatira zolondola komanso zodalirika. Mwa kumvetsetsa mwamphamvu magwero a kulowererapo ndi kukhazikitsa njira zogwirizira zogwiritsira ntchito, ma laboratoes amatha kuchepetsa mphamvu za zinthu zakale zokhudzana ndi ma chromatographic. Mwa njira yosinthira bwino, njira zokhazikika, komanso kukhathamiritsa, kusanthula, ndi kubereka kwa miyeso ya ma chromatographic, potsatira mafakitale, ndi mafakitale.

Tsegulani mayankho 50 apamwamba onena za HPLC Vials m'nkhaniyi. Pezani chidziwitso ndi chidziwitso chofunikira kwambiri kuti mudziwe zambiri zanu za chromator. Dziwani tsopano !: 50 mafunso ambiri omwe amafunsidwa kawiri pa HPLC Mbale

M'mbuyomu:

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Ena:

Kuthana ndi nkhani zosagwirizana pakati pa zosefera ndi zitsanzo zofewa

Kuthana ndi Kusokonekera Kumtsogolo Mbale

Chiyambi