Kusankha Buffer yoyenera kwa gawo lanu la HPLC ndikofunikira kuti mukwaniritse zolekanitsa kwambiri ndi kusanthula kwanu zitsanzo. Buffers imathandizira kusunga ph ph, yomwe ndiyofunikira pakuwongolera mkhalidwe wa oionization, motero zimakhudza nthawi yawo yosunga komanso mawonekedwe ake. Pansipa pali kufotokozedwa mwatsatanetsatane momwe mungasankhire buffer yabwino kwambiri pa HPLC yanu.

Kodi mukudziwa za kukonzekera kwa HPLC zitsanzo? Dinani nkhani iyi kuti muphunzire zambiri: "Chilichonse chomwe muyenera kudziwa za kukonzekera kwa HPLC zitsanzo"

Kufunika kwa kusankha kwa buffer

Buffer ndi yankho lomwe phwi silisintha mutatha kuwonjezera acid ochepa kapena maziko. Kutengera ndi kapangidwe kake, mabiani amatha kukhala olembedwa ngati acidic kapena oyambira. Kugwira ntchito kwa buffer kumatsimikiziridwa ndi PKA yake, yomwe iyenera kukhala mkati mwa ± 1 p3 p3 p3 pH yomwe mukufuna kuti gawo la PH lomwe mukufuna.

Zinthu zazikulu zofunika kuziganizira

1.P Quomes: the PH of the teple iyenera kukhala magawo awiri a PHA kutali ndi PKA ya chiwerewere. Izi zimathandiza kupondereza isoni ya acidic kapena oyang'anira, kukulitsa kusungidwa kwawo panjira. Mwachitsanzo, ngati kafukufukuyu ndi acid ndi pka ya 4.5, pogwiritsa ntchito gawo lam'manja ndi PH kuzungulira 2.0 angalitseke mu boma lomwe silinayankhe, zomwe zimathandizira.

Mtundu wa 2.Biffer: Zovala wamba zomwe zimagwiritsidwa ntchito ku HPLC zimaphatikizapo:

Buffer (zothandiza mu pH magawo 2.0 mpaka 8.0, ma gferser a phosphate amagwiritsidwa ntchito kwambiri chifukwa cha kukhazikika kwawo komanso kuphatikizidwa ndi ambiri. Amakhala oyenera makamaka kwa mapulogalamu a LC-UV.

Acetate Buffer: Izi ndizodziwikanso kwa LC-UV, ndipo zitha kugwiritsidwa ntchito ngati ma ph imachitika monga phosphate biffer.

Kusinthasintha kwa Volalale: Kwa LC-MS Mapulogalamu, osinthika, acetate, ndi ammonium bicarbonate chifukwa amatha kuchotsedwa mosavuta panthawi yowoneka bwino.

3.buffer: Kuyambitsa kukhazikika kwa biffer ku HPLC kuli pakati pa 25-50 mm. Kukhazikika kwamphamvu kumatha kusintha mawonekedwe a mchimodzi ndi kuchita bwino koma kungayambitsenso mpweya ukasakanikirana ndi ma soric. Ndikofunika kusunga buffer pansi pa 25 mm kuti mupewe zovuta.

Chiyero cha 4.Chemical choyera: Nthawi zonse muzigwiritsa ntchito ma chromatographic-giredigraph-girfield yoletsa kuipitsidwa ndikuwonetsetsa zotsatira zodalirika. Zosakhazikika zimatha kubweretsa zosayembekezereka komanso kusiyanasiyana nthawi zonse.

Zotsatira zake: Kutentha kumatha kuwongolera ma pH ya zothetsera bufer. Ndikofunikira kukonzekera ndikusunganso kutentha kutentha kosasunthika kuti athandize kwambiri.

Ndikufuna kudziwa mayankho 50 onena za HPLC Vials, chonde onani nkhaniyi: 50 mafunso ambiri omwe amafunsidwa kawiri pa HPLC Mbale

Malangizo othandiza pakukonzekera kwa buffer

Njira Yokonzekera: Zovala zitha kukonzedwa ndi zosakanikirana za acimor acidic ndi zoyambira kapena zochokera pakugawana ndi acid. Pewani kusintha pH mutawonjezera ma sodic, chifukwa izi zimatha kubweretsa zotsatira zosagwirizana.

Mayankho Atsopano: Konzani mayankho atsopano ophatikizira pafupipafupi kuti muchepetse kukula kwamankhwala komanso kuwonongeka kwa zinthu zina, zomwe zingakhudze ma chromatographic magwiridwe antchito.

Kupewa Trussion: Biffers, monga Citrate, amatha kusenda dzimbiri ndi zigawo zosakanikirana za HPLC. Onetsetsani kuti mwakusintha kwa dongosololi mutatha kugwiritsa ntchito zingwe zotere kuti zisawonongeke.

Mapeto

Kusankha buffer yoyenera kwa gawo lanu la HPLC ndikofunikira kuti mukwaniritse zotsatira zodalirika komanso zobereka. Ganizirani za Ph, mtundu wa Buffer, kuphatikizira, ndi chiyero posankha. Potsatira malangizo awa, mutha kukonza njira yanu ya HPLC yolekanikirana ndi kusanthula zitsanzo zanu.

Ndikufuna kudziwa mtengo wa HPLC Vials, chonde onani nkhaniyi: HPLC Vials Mtengo: 50 mafunso ambiri omwe amafunsidwa nthawi zambiri

M'mbuyomu:

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Ena:

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