Chromatography ndi njira yodziwika kwambiri yodziwika m'machesi yowunikira yomwe imagwiritsa ntchito kulekanitsidwa ndi kusanthula kwa zosakaniza zovuta. Kupambana kwa njira za chromatographic kumatengera zinthu zosiyanasiyana; Gawo limodzi lotere ndi lamiyala ya chromatography, yomwe imachita gawo lalikulu posunga umphumphu ndi kupanga zolondola. Apa tikuwona zonse muyenera kudziwa za zinthu zomwe zimagwiritsidwa ntchito pazotengera izi ndikuwonetsetsa zotsatira zolondola.

1. Mbale zagalasi

Mbale za GLPLC HPLCkwakhala nthawi yayitali kuntchito chifukwa cha kukana kwa mankhwala apadera ndi kusiyanasiyana. Galasi la mabotolo, njira imodzi yotchuka, imakhala yofunikira kwambiri yowonjezera yowonjezera yolimbana ndi maliro omwe amathandizanso pakukonzekera mitundu kapena kusanthula komwe kumadalira deta ya kutentha. Kuphatikiza apo, kukhazikika kwa mankhwalawa kumatsimikizira kuti sizingayambitse zodetsa nkhawa zomwe zimasokoneza njira zolekanitsira ngati malo olekanitsidwa; Galasi la lamba laimayi lingapatukenso kutsutsana kwenikweni kuposa mnzake.

Delve mu ukulu wa Mzere wa Zigamba za galasi pa mapulasitiki. Pezani mayankho mu nkhani yathu yochitira umboni:Zifukwa zitatu zapamwamba zomwe zimapangidwira miyala chromatography ndiyabwino kuposa Mbale zapulasi

2. Mbale zapulasitiki

Mtengo wa pulasitiki wa pulasitikiapeza kutchuka chifukwa chogwiritsa ntchito mtengo wake, chibadwa chopepuka komanso kutaya. Polypropylene ndi mapulaneti a polyplenene ndi zosankha zotchuka mukamapanga migolo ya pulasitiki; Onsewa atha kupereka zabwino pankhani yoteteza kuteteza ma ions kuchokera ku yankho, ndikuwapangitsa kukhala koyenera kugwiritsa ntchito zina; Komabe ayenera kugwiritsidwa ntchito mosamala mukamagwiritsidwa ntchito pamatenthedwe okwezeka ngati mapulaneti ena sangapirire kutentha kumeneku.

Dziwani zifukwa zomwe zasankha zasankha zamiyendo. Pitani patsamba lathu kuti muzindikire lingaliro lofunikirawu:Kupindula kwa Kugwiritsa Ntchito Masamba a Chromebography

3.. Polytetrafluoro solmblene (PTF) yokutidwa

Zovala za PTF zimachita mbali yofunika kwambiri pakuchepetsa ziwonetsero zokhala ndi mipata. Malo awo osagwirizana amachepetsa chiwerewere pa malinga a vial, motero kuchepetsa kutaya ndikukwaniritsa zolondola. Izi ndizofunika kwambiri mukamathana ndi kusanthula kwamiyendo yomwe mwina mphindi zisanthu zomwe adsortesh zimatha kukhala ndi makonzedwe owopsa pazotsatira; chifukwa chakeMbale zokhala ndi PTFFKuchulukitsa kudalirika komanso kumverera nthawi ya chromatographic.

4. Silicone septa

Sepga, kapena zisoti, zopangidwa ndi silika kawirikawiri zimapezeka mu chromatography mphete chifukwa cha chilengedwe chake, kusinthasintha komanso kulimba. Silicone imapereka chisindikizo chodalirika chomwe chimaletsa kuipitsidwa poteteza zitsanzo zotsutsana ndi njira yowunikira - zinthu zonse zofunika kwambiri pakusunga umphumphu. Mphamvu zawo zowonongeka zimathandizira kugwiritsidwa ntchito mobwerezabwereza mukamasungabe Chisindikizo cha asirikali, kuwonjezera moyo wanthawi zonse cemomography.

Mukufuna kudziwa zambiri mu PTF \ / Silicone septa? Osayang'ananso! Onani nkhani yathu kuti mumvetsetse bwino zinthu izi:Chilichonse chomwe muyenera kudziwa: 137 Pre-slit ptfe \ / slika slicone faps

5. Kukula kwa vial ndi mawonekedwe

Mbale za chromatography zimabwera mosiyanasiyana komanso mawonekedwe osiyanasiyana kukakumana ndi zosowa zosiyanasiyana. Zofananira zofananira za chizolowezi cha chizolowezi cha 1.5 ml ndi 2 ml ndi mipanda iwiri, ngakhale mbale zokulirapo kapena zazing'ono zimatha kusankhidwa kutengera zofuna za zitsanzo zowunikira. Mbale zomwe zimakhala ndi zitsulo zokhala ndi zosokoneza kapena zathyathyathya zomwe zimapangitsa kuti zisamaliro zamitundu yolekanitsa - zomwe ofufuza ziyenera kuganizira ntchito yawo kuti ithetse zotsatira zopatukana kwambiri za chromatographic.

6. Chitsimikizo ndi ulamuliro wapamwamba

Chitsimikizo chaMbale za HPLCndi chinthu chofunikira pakutsimikizira kuyenera kwa ntchito yogwiritsa ntchito mosamalitsa. Madenti Omwe Amatha Kupanga Mayiko a United States (IP) ndi Europe) amakhazikitsa Miyezo Yogwiritsa Ntchito Mbiri Yotsika Kwambiri

Ikani mu nkhani yathu kuti mupeze ntchito 15 zamitengo ya chromatography. Tulutsani kusinthasintha kwa zida zofunikirazi posanthula molondola:Mapulogalamu 15 a Chromatography Vials m'minda yosiyanasiyana

M'kati yovuta ya chromatographraphraphry, yomwe imagwiritsidwa ntchito pomanga vial imachita mbali yofunika kwambiri pabwino. Galasi ndi pulasitiki ya pulasitiki, zokutira za PTF, ndiSilicone septaChilichonse chomwe chimakulitsa chomwe chimakulitsa kulondola, chidwi, komanso kudalirika panthawi yowunikira. Monga ofufuzawo amasankhira zosankha zawo pakati pawo, kumvetsetsa zinthu izi, zomwe zimagwiritsidwa ntchito ndikusankha galasi labwino - zosankha zamapulasitiki zamakono - zogwiritsira ntchito pulasitiki yamakono - kuti mugwiritse ntchito. Ofufuzawo amatha kupanga zisankho zanzeru omwe ali ndi zotsatirapo zolimbitsa thupi zotsatira zoyipa pazinthu zawo zowunikira.

Tsegulani chidziwitso 50 mu HPLC Mbale! Fufuzani Nkhani Yathu Yoti Mayankho a Mafunso Anu Oyalidwa:50 mafunso ambiri omwe amafunsidwa kawiri pa HPLC Mbale

M'mbuyomu:

Chifukwa chiyani mipata yapamwamba imagwiritsidwa ntchito mu chromatography? Zifukwa 5

Ena:

Zifukwa 10 Zomwe Muyenera Kugwiritsa Ntchito PTFE Mbale Za Chromatography