Kodi mukudziwa bwino ma chromatography.

. HPLC ndi chida champhamvu chowunikira chomwe chimagwiritsidwa ntchito poyeretsa, kudzipatula, kuzindikira, komanso kuchuluka kwa chisakanizo.

Kuchuluka kwa ma chromatographyChida chimakhala ndi magawo am'manja, mapampu, jekeseni, zigawo zolekanitsa, ndi zofufuza. Zinthu zomwe zimasungidwa ndi kupompa gawo lamadzimadzi (zosungunulira) zosakanizidwa ndi zitsanzozo kukhala nambala yodzaza ndi gawo lokhazikika. Gawo lirilonse la osakaniza limabweratse mosiyanasiyana ndi zozama za zinthu, kulekanitsa zigawo za zinthu zomwe zitsanzozo ndikuzichotsa pachilichonse nthawi zosiyanasiyana. Choyamba, zinthu zomwe zimalumikizana bwino ndi gawo lamadzi limatuluka.
Kenako, zinthu zomwe zimagwirizanira ndi gawo lolimba limatuluka. Kuchuluka kwa ma chromatography imagwiritsidwa ntchito kuyeza ndi kuyeretsa zomwe zili m'malo osiyanasiyana a zitsanzo za zitsanzo. Mwachitsanzo, itha kugwiritsidwa ntchito m'makampani opanga mankhwala. Mu malonda awa, HPLC ndiyothandiza kwambiri pakuyeza kuchuluka kwa mankhwala othandizira m'magawo ena.

Pafupifupi 90% yonse Kuchuluka kwa ma chromatography Makina amagwiritsa ntchito chowongolera cha UV kuti muyeze chidwi chazomwe zimawoneka ngati zowoneka bwino. Kukhazikika kumapezeka kudzera mu lamulo la mowa wa lambat: A = ε L L. Chojambulacho chimayesa kuyanjananso nthawi imodzi kapena zingapo. Pali mitundu itatu ya zofufumitsa.

Cholembera chimodzi ndi zosefera zowoneka bwino, chojambula chosakanikirana chokhala ndi kukazima ndi mavekeki angapo divekth dided-array (abambo). Ibsen adapatsa Abambo Omwe ndi mawonekedwe abwino kwambiri komanso omwe amasintha kwambiri.

Ngati muli ndi funso Kuchuluka kwa ma chromatography, kapena mukufunaMbale za chromatography, chonde funsani nafe pompano!