2. Kumva kwambiri kwa HPLC yanu ya HPLC
3.. Kulondola Kwambiri
4.. Nsonga zomveka zopanda tanthauzo
5. Mfuu pang'ono zakumbuyo
6. sakuthana
Njira Zinayi Zokonzekera HPLC
Tiyeni tikumane momwe mungasungire nthawi ndi mayankho athu ndipo nthawi yomweyo imakulitsa tanthauzo la zotsatira zanu:
Gawo 1. Kusanthula kwa mayankho am'madzi
Ubwino wamadzi osauka ndi chimodzi mwazinthu zomwe zimathandizira pamavuto pa nthawi ya HPLC. Zosayipa zamadzi mu gawo lam'manja zimatha kulumikizana ndi mzati ndikupangitsa kuti zisakhale zosayenera komanso chidwi chosayembekezereka kapena nsonga zosayembekezereka mu chromatogram, yomwe nthawi zambiri imatchedwa nsonga za Ghomit. Kugwiritsa ntchito madzi kumachepetsa chiopsezo cha kuipitsidwa ndi mabisala organic, tizilombo tating'ono, Endotoboxins, ma RANases, ndi Donase. Madzi a utoto amapezeka kuti agule, pogwiritsa ntchito dongosolo loyeretsa madzi mu labotale yanu ndi njira yosavuta kwambiri yotsimikizira madzi apamwamba kwambiri. Lembani madzi 1 omwe amakumana ndi Assom, NCCLS, ISO, ndi USP muyeso ndi chisankho chabwino kwambiri pa ntchito za HPLC.
Gawo 2. Kukonzekera miyezo
Kukonzekera miyezo ya kuzindikiritsa ndi kuchuluka kumatha kukhala njira yotopetsa komanso yophulika nthawi. Kuyeza molondola kuti kusinthika kwa zosungunulira kungagwiritsidwe ntchito ndizovuta, ndipo zinyalala chifukwa cholakwitsa, makamaka mukamagwira ntchito ndi mankhwala osokoneza bongo. Zinthu zina monga kutentha zimagwirizanitsa kukonzekera koyenera. Kusasinthasintha Kusintha kwa kutentha kumasintha kachulukidwe kameneka komanso kumapangitsa kuti pakhale kusiyana kwakukulu pakukhazikika.
Kugwiritsa ntchito moyenera zomwe zimapangitsa kulondola kulondola kwa malo angapo kuti muchepetse ndalamazo polumikiza ndalama zolipiritsa zomwe zimatsimikizira kuti ndizosagwirizana kwambiri. Kugwiritsa ntchito mapulogalamu monga Yapp16 Dosing Q-App imathandizira kuwerengera kokha ndi kuwunikira kwa gravimetric koyeza. Kulemba kutentha ndi kuchuluka kwa zosungunulira zimathandizanso kukhala olondola kwambiri, makamaka pokonzekera miyezo pamasiku otsatira. Kutumiza kwa data ku Yapp16 Kuyeza kwa thermon ™ cromemeleon ™.
Gawo 3. Bweretsani zoyambira ndi mapaipi abwino
Kufunika kwa kusapaka kolondola komanso kolondola nthawi zambiri kumayikiridwa kwambiri komanso kuwunikira. Luso la ogwiritsa ntchito ndi zokumana nazo zitha kukhala ndi tanthauzo lalikulu kwa owunika ngati HPLC. Kusankha piperi yoyenera imatha kuchepetsa zolakwitsa za anthu ndi ndalama zomwe zidagwirizanitsa zowonongeka.
Mapaipi achikunja omwe ali ndi chotupa cha voliyumu amapereka njira yosavuta yopezera kusintha kwamphamvu kwa mwangozi, pomwe mapimu amagetsi ambiri amachotsa kusintha kwa ogwiritsa ntchito moyenera. Chinsinsi cha pipette ndichimodzinso chinthu chofunikira kuganizira za HPLC. Malangizo ocheperako ndi abwino pogwira ntchito ndi ma virus osiyanasiyana, pamene amakulitsa chikondwerero chachitsanzo. Kuyipitsa komwe kumathanso kumachitika chifukwa chokweza nsonga ya pipette. Mwakutero, maupangiri ndi mankhwala osokoneza bongo amasunga zitsanzo.
Gawo 4. Fyuluse kuti muonera bwino komanso kukhala ndi moyo wautali
Kusamalira zofananira ku kusefa zitsanzo musanawonetsere kudalirika ndipo kumatha kukuthandizani moyo wa HPLC. Zosefera syringe ndi njira yosavuta, mwachangu yosefera zitsanzo musanakhale columnography. Monga nsonga za pipette, ma sol solc amagwiritsidwa ntchito ku HPLC imapangitsa kuti pakhale zosefera zina kuti mupewe kugwedezeka ndi kuipitsidwa.
Cellulose nembani amagwirizana ndi dimethyl muluxide (DMSO), Amide, matomoni, ester, ndi ma erther. Pazomwe zimapha kwambiri, kuwulula Polytaslulfone (PTFO) Jembranes kupewa kutulutsa ndi kuyipitsidwa. Kuseferera kwachitsanzo kumayamba nthawi yayitali pomwe nambala ya zitsanzo imachuluka. Magawo a mitundu yambiri ya kufesa, monga njira yofala ®, amatha kukonza zitsanzo popanda syringes ndi njira yofulumira yokonzekera popanda kupulumuka.
Mavuto asanu muyenera kulabadira!
1. Kodi ndi zitsanzo zazikulu ziti zazikulu zomwe zikuyenda pamavuto?
Laboratoes akuwonjezereka pakukakamiza nthawi ndi ndalama, zomwe nthawi zambiri sizimangochita zambiri zokha komanso zitsanzo za mawu osiyanasiyana. Kukonzekera kwa zitsanzo ndi malo omwe pali kusiyana pakati pa mitundu ya zitsanzo kapena njira zotsatsa ndi zida zogwiritsidwa ntchito zomwe zimagwiritsidwa ntchito zingakhudze zotsatira zake. Kuwonetsetsa kusiyana pakati pa zida zomwe zili mu chipangizo chofanizira ndipo zosungunulira zitha kuchepetsa zovuta; Mwachitsanzo, kuchuluka kwa zowonjezera zomwe zingasokoneze kupezeka kwa kafukufuku.
2. Chimachitika ndi chiani pamene Samp Pres Presp ikulakwitsa?
Asanawunikire bwino kwambiri ma chpomatography (HPLC), zitsanzo zimakonzedwa ndi njira zosiyanasiyana, zomwe zonse zitha kusintha zotsatira zowunikira pokhudza zitsanzo zomwe zimalowetsedwa m'dongosolo. Kukonzekera kwachilendo kosagwira mtima kumatha kutsika munthawi ya chipilala, kuchuluka kwa ntchito za ntchito, kutaya kwa zida, kuchepetsa kwa chromatographic njira yothandiza komanso kubereka.
3. Poyamba kuyang'ana, kusefa kumawoneka ngati njira yosavuta ...
Ngakhale kusefa ndi gawo laling'ono la ntchito yayikulu, zomwe zimapangitsa kuti posankha fyuluta yoyenera chifukwa chogwiritsa ntchito kungakhale kwakukulu. Mwachitsanzo, njira zomwe sizimachotsa nkhani yokwanira tinthu tating'onoting'ono zitha kutsimikiza. Kusankha kosayenera kwa zida zokonzekera zitsanzo zitha, kumasulidwe pazomwe zimachitika mu zitsanzo, zomangika chidwi ndi zomwe zimapangitsa kuti zikhale zotayidwa - zimapangitsa kuti mitundu yonseyi ichitike. Ngati mukukhulupirira kuti zosemphana zanu sizimapereka zolakwika zomwe zimafunanso kukonza kapena kuwononga ndalama, mudzasunga ndalama ndikudzipatsa mtendere wamaganizo.
4. Kodi zitsanzo zofala kwambiri 'zalephera'?
Kusankha kolakwika kwa Meferane ndi vuto limodzi lomwe timawona kwambiri. Mavuto amatha kuwoneka ngati nsonga zakunja chifukwa chochotsa, zomwe zimatha kusokoneza kuzindikira kwa chidwi. Kusagwirizana kwa zosefera ndi zitsanzo kapena zosungunulira malinga ndi kukana kwa mankhwala sikungokhala kofatsa kwa zosefera. Ma sol sol sol angapangitse kutupa pang'ono polymeric membrane pomwe siyingagwirizane kwathunthu, zomwe zimapangitsa kuti kuwononga magwiridwe antchito, kumapangitsa kuti zisakhale zoyera. Njira imodzi yothetsera vutoli ndikusankha nembanemba yomwe imagwirizana kwambiri ndi aquous komanso okhazikika. Kwa HPLC zitsanzo za HPLC zitsanzo, syringe ndi zosefera zopanda stripsi zomwe zimakhala ndi ma celluune obwezeretsanso ndi njira yabwino yosungunulira.
Kugwiritsa ntchito chipangizo chosefera chomwe ndi chachikulu kuposa kungokonzekera kuchuluka kwa voliyumu kumayiko ena wamba. Kugwiritsa ntchito zida zazikulu kwambiri kumatanthauza kuti zitsanzo zochepa zimakhalabe mu zosefera, ngakhale mosamala zimatengedwa kuti zithandizire momwe zingathere.
5. Ubwino wotenga gawo ndi uti?
Cholinga chachikulu cha malobories ambiri omwe amayendetsa masewera olimbitsa thupi amayesa ndikupanga njira zosasinthika zomwe zimasasinthasintha - ndipo zimafuna zosefera mosasinthasintha. Kusankhidwa kwa chipangizo chojambulidwa ndi kukula kwa cholumikizira ndikusunga komwe kumathandizira kuchotsa kwa tinthu tating'onoting'ono, pomwe kusankha kwa kukula koyenera kumatha kuchepetsedwa kuchepetsedwa. Kufalikira kwabwino kumathandizanso kuteteza mzatiyo ndikuwonjezera nthawi yonse ya jekeseni ndi mzati.
Mapeto
HPLC ndi njira yofunika kwambiri pa sayansi yamoyo komanso kafukufuku wa biopharaceutical momwe amaperekera zotsatira zolondola, zopangidwa. Izi zimatengera kukhulupirika kwa HPLC chitsanzo kwa HPLC, komanso njira yabwino kwambiri yowonetsetsa zitsanzo zapamwamba ndikuyamba ndi zida zabwino kwambiri. Ndi mayankho a madzi okwanira, kukonzekera kofanana, ma pipettes, maupangiri, ndi zosefera, njira ya HPLC ya Quppy Quoment iliyonse. Mayankho ndi mapulogalamu othandiziranso amathandizanso kulera zitsanzo pokonzekera kupereka zitsanzo zoyera kuti zitheke.
Zokambirana zambiri za HPLC zitsanzo zokonzekera bwino zomwe zalandiridwa!
Achizungu
Zauchina
