Makina oyendetsa bwino a chpmutography (hplc) ndi ma chromatography - misa spectometry (ma lcms) ndi njira ziwiri zamphamvu zomwe zimagwiritsidwa ntchito m'mitundu iwiri, kuphatikizapo ma sayansi ya zamankhwala, sayansi ya chilengedwe, ndi sayansi yamoyo. Ngakhale njira zonse ziwiri zimaphatikizira kulekanitsidwa ndi kusanthula kwa mankhwala, mfundo zake, ntchito zawo, zomwe zimagwiritsidwa ntchito ndizosiyana kwambiri. Ntchito ya blog iyi iyerekeza HPLC ndi LCMS, kuyang'ana kusiyana kwake ndi zabwino za luso lililonse.

Mfundo yofunika kugwirira ntchito

Hplc

HPLC ndi njira yachitsulo yomwe imalekanitsa mankhwala ophatikizika malinga ndi momwe amachitirana ndi ma station ndi mafoni. Ku HPLC, gawo lamadzi lamadzi limadulidwa kudzera mundime yodzaza ndi gawo lakale, lomwe limakhala ndi tinthu tating'ono ta silika kapena ma polica. Mukamasakaniza njira yosinthira imayambitsidwa m'dongosolo, zigawo zikuluzikulu zimasiyanitsidwa kutengera ubale wawo chifukwa cha gawo losachedwa. Mafuta omwe amalumikizana kwambiri ndi gawo la masitimalo pang'onopang'ono, pomwe amaphatikiza kuti amalumikizana kwambiri. Mafuta olekanitsidwa amapezeka pogwiritsa ntchito zowunikira zosiyanasiyana, monga UV-Wy-Wy-Fluorescence, kapena Refordive Resorx.

Mukufuna kudziwa zambiri za mapulogalamu a Mbiri ya Chromatography, chonde onani nkhaniyi:Mapulogalamu 15 a Chromatography Vials m'minda yosiyanasiyana

LCMS

LCM imaphatikiza kuthekera kwa HPLC ndi kuchuluka kwa kuchuluka kwa spectrometry. Mu LCMS, zitsanzo zimasiyanitsidwa koyamba pogwiritsa ntchito HPLC, monga mu HPLC. Komabe, mmalo mokonzedwa ndi cholembera wamba, zinthu zomwe zamveka zimayambitsidwa mu mawonekedwe a misa. Mafuta akuluakulu amachititsa zinthu ndikupanga kuchuluka kwake kwapadera, kufotokozera mwatsatanetsatane zochulukitsa ndi kapangidwe kake kake. Kuphatikiza kwa kupatukana ndi misa spectrometry kumapereka chidwi kwambiri komanso mwachindunji kuposa HPLC yokha.

Kupatukana

HPLC:HPLC imagwiritsa ntchito kukakamizidwa kwambiri kuti mukankhe gawo lamadzimadzi kudzera pamtundu wokhazikika wokhala ndi gawo lokhazikika. Mafuta amalekanitsidwa kutengera njira zawo zosiyanirana ndi gawo lakale, zomwe zimapangitsa kuti kupatulize koyenera.

LCMS:LCM imaphatikiza kuthekera kwakuthupi kwa HPLC yokhala ndi maluso a misa ambiri a Spectombingry (ms). Kanthu kamene kamadzimadzi kwa chromatography kumasiyanitsa mankhwala, pomwe misa imayesa gawo lalikulu la mitundu yaidired.

Chidwi ndi kusankhidwa

HPLC:HPLC ili ndi chidwi komanso kusankhidwa bwino, makamaka ngati zojambula zapamwamba monga UV-Wy-Fluorescence, kapena daid gray. Komabe, HPLC yokha singapatse mawonekedwe okwanira zitsanzo zovuta.

LCMS:LCMS ili ndi chidwi chapamwamba komanso kusankha poyerekeza ndi HPLC. Mawonekedwe akulu amatha kudziwa bwino komanso kuchuluka kwa mankhwala malinga ndi zigawo zawo zapadera zoti azingokhalira, ngakhale pama matricts zovuta. Izi zimapangitsa kuti LCMS makamaka ndizothandiza pakusanthula ndalama ndi kutsimikizira zomwe sizikudziwika bwino.

Kukonzekera zitsanzo

HPLC:Kukonzekera zitsanzo za HPLC nthawi zambiri kumaphatikizapo kuchepa, kusefa, kapena njira zosavuta. Zitsanzo siziyenera kutulutsidwa, motero HPLC ndiyoyenera kuwirikiza mitundu yambiri, kuphatikizapo polar ndi osagwirizana.

LCMS:Kukonzekera kwa LCMS kumafanana ndi kuti kwa HPLC, koma zowonjezera zingafunikire kuonetsetsa kuti kuyeserera ndi misa misa. Mwachitsanzo, zowonongeka zosasunthika kapena zowonjezera zitha kuyenera kuwonjezera mankhwala ndikuletsa Ian Ian.

Mapulogalamu

HPLC:HPLC imagwiritsidwa ntchito kwambiri pakuwunika kwa mankhwala a mankhwala, zakudya, ndi zitsanzo zachilengedwe. Zimakhala zothandiza kwambiri kupatukana ndi zochulukirapo pamasamba ovuta, monga zosanjidwa ndi mankhwala, zowonjezera zakudya zowonjezera, komanso zotsalira za mankhwala ophera tizilombo.

LCMS:LCMS ili ndi mapulogalamu angapo, kuphatikiza:

Kusanthula kwa mankhwala: Kugwiritsidwa ntchito popezeka mankhwala osokoneza bongo, chitukuko, komanso kuwongolera kwapadera.

Ma protefoomic ndi metabolomics: ankakonda kudziwa komanso kuchuluka kwa mapuloteni ndi ma metabolites pazitsanzo zachilengedwe.

Kusanthula kwa chilengedwe: Kugwiritsa ntchito kuzindikira zodetsa nkhawa m'madzi, nthaka, ndi zitsanzo za mpweya.

Chofunika Kwambiri Zokhudza Udindo Wotsimikizika Wotsimikizika HPLC Poonetsetsa kusanthula kosakwanira? Werengani nkhaniyi: Kufunika kwa Mbale Zotsimikizika za HPLC ya kusanthula kolondola

Mapeto

HPLC ndi LCMs ndi njira zonse zamphamvu zowunikira ndi mphamvu zawo ndi zofooka zawo. Kusankha pakati pa awiriwo kumatengera zofunikira mwatsatanetsatane za kusanthula, monga kukhudzidwa, kusanza, zitsanzo zovuta, komanso zida zopezeka.

HPLC ndi chisankho chabwino chopenda mosanthula zinthu zodziwika bwino pama matrics osavuta, pomwe mtengo komanso mosavuta kugwiritsa ntchito ndizofunikira. Nyimbo, kumbali inayo, njira yomwe ndimakonda kwambiri yopenda zitsanzo zovuta, kuzindikiritsa zinthu zosadziwika, ndikukwaniritsa zowunikira za ultra-trace.

Monga ukadaulo ukupitilizabe kupitiriza, kuphatikiza kwa HPLC ndi LCMS kudzatenga gawo lofunikira kwambiri pakukumana ndi zosowa zapamwamba za chemistry ndikuwonetsetsa kuti zinthu zapamwamba pazinthu zosiyanasiyana pamagwiritsidwe.

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