Kupanga mankhwala opanga mankhwala kumafuna njira zowongolera zowongolera kuti zitsimikizire chitetezo, chomwe chimatha, komanso kusasinthika mankhwala omwe anthu mamiliyoni ambiri amagwiritsa ntchito tsiku lililonse. Chromatography imagwira cholinga chabwino apa, polekanitsa, kuzindikiritsa, komanso kuchuluka kwa zigawo zingapo zosakanikirana. Mitengo ya chromatography imagwira ntchito yofunika kwambiri munjira yowunikirayi popereka malo olamulidwa kuti awerengere ndi kutetezedwa - Nkhaniyi imakhudza mwakuya mphamvu zawo zoyendetsera makina.

Chromatography mu mankhwala: mwachidule

Chromatography amatanthauza njira zingapo zomwe zimagwiritsidwa ntchito kupatula zosakanikirana ndi magawo awiri omwe ali ndi magawo awiri - nthawi zambiri amakhala okhazikika pa gawo lokhazikika) ndi mafuta omwe amanyamula zigawo za ilo).

Makina oyendetsa bwino a chvatotography (hplc) ndi gasi chromatography (GC) ndi njira ziwiri zogwirira ntchito zamankhwala opanga mankhwala pomwe GC imagwiritsidwa ntchito bwino posanthula zinthu zomwe zingagwiritsidwe ntchito popanda kusokonekera. Njira zonsezi zimafuna kuwongolera, kubereka, komanso kulondola komwe kungakwaniritsidwe kudzera mu zitsanzo zovomerezeka ndi zizolowezi.

Kukulitsani syringe yanu kumasefedwa ndi kuzindikira kuchokera ku nkhani yothandizayi. Osaphonya!Mutu wa "syvinge sfaw" 50 nthawi zambiri mafunso

Kugwiritsa ntchito mtunda wa chromatography mu mankhwala oyendetsa bwino

Mbale za chromatography ndi zotengera zomwe zimapangidwa makamaka kuti zigwiritsidwe ntchito pogwiritsa ntchito njira za chromatographic, chifukwa zimapangitsa kulondola komanso kudalirika kwa zotsatira zowunikira. Nawa zifukwa zingapo zopitilira cromatography ndizofunikira:

Kukhulupirika:Zoyimira mankhwala zimatha kumverera makamaka zachilengedwe ngati kuwala, mpweya ndi zodetsa nkhawa. Mitengo ya chromatography imapereka malo opangira mpweya ndi kuwala, kuteteza zitsanzo zowonongeka pakusungira ndi kusanthula.

Kuchepetsa kuipitsidwa:Ngakhale kuchuluka kwa zodetsa kumatha kusokoneza zolakwa za zotsatira zake, kotero pogwiritsa ntchito Mbale zopangidwa ndi zigalasi za borosiltific boll amathandizira kuchepetsa zoopsa zomwe zidadetsedwa.

Kugwirizana:Mbale ziyenera kupangidwira mwachindunji kwa mtundu wa zitsanzo zomwe mukufunsidwa. Mwachitsanzo, zitsanzo zosasunthika kusanthula kudzera pa GC zimafuna milomo yomwe imachepetsa kuyanjana pakati pa zitsanzo zawo ndi zoyipa kuti asatenge mamolekyulu pamtunda ndikuletsa Adsorption; Zitsanzo za HPLC zimafunikira MBIRI Zomwe sizili zowonjezera mu zitsanzo zawo zomwe zingasokoneze kusanthula.

Zitsanzo za Kusintha:Kusanthula, zitsanzozi zingatuluke powunikira, zomwe zimayambitsa zolakwika. Mbale zomwe zidapangidwa ndi ziwonetsero zoyenera ngati zikopa zokhala ndi septa zitha kuletsa zitsanzo za kusintha ndikuwonetsetsa kusanthula.

Onani nkhani iyi kuti mudziwe bwino maluso a HPLC ndikuwonjezera luso lanu la chipani: Mayankho a HPLC Kukonzekera kwa Zotsatira Zabwino

Kusinthanitsa ndi Kubala:Kusasinthika ndi kubereka kwa zotsatira mu chiwongolero cha mankhwala osokoneza bongo ndikofunikira kwambiri. Mbale zam'matumbo zoyenereratu zimatsimikizira kuti zitsanzo zimasanthula mofananamo, motero kuchepetsa kusiyanasiyana kwinaku akuwonetsa zotsatira zodalirika.

Kugwirizana kwa OVUER:Ndi mphamvu ya labotale pa kukwera, Mbale za chromatographler zapangidwa kuti azigwira ntchito mosadukiza ndi makina ogwiritsa ntchito makina ogwiritsira ntchito bwino ndikuchepetsa chiwopsezo cholakwika cha anthu.

Mapeto

Kuwongolera kwa mankhwala kwa mankhwala ndi gawo limodzi lopanga mankhwala osokoneza bongo, kuwonetsetsa kuti mankhwala ndi otetezeka, othandiza, komanso osasinthasintha. Mtengo wa chromatography umakhala ndi gawo lofunika kuteteza zitsanzo pakuwunika pogwiritsa ntchito malo opanda mawonekedwe, osuta, kapena kuwonongeka - kupititsa patsogolo kulondola komanso kudalirika panthawi yowunikira. Ndi kupita patsogolo kwaukadaulo kumabwera mozungulira, Mbale izi zisinthana kwambiri kuti akwaniritse kusintha kwa mankhwala osokoneza bongo omwe amafunidwa ndikugwiritsa ntchito njirayo m'njira.

DZIWANI MAYO WA MAFUNSO OGWIRITSIRA NTCHITO 50 HPLC m'nkhaniyi, kuvumbula kuzindikiritsa kwa malingaliro a chidziwitso:Mapulogalamu oyendetsera masewera olimbitsa thupi a mankhwala a chromatography

M'mbuyomu:

Kupindula kwa Kugwiritsa Ntchito Masamba a Chromebography

Ena:

Kodi mizere ya 2ml HPLC imatumizidwa bwanji ndikusungidwa?