Chromatography ndi chida chofunikira kwambiri pakufufuza zasayansi ndikuwunika, kulola kupatukana, kudziwitsa, ndi kuchuluka kwa zosokoneza zovuta. Ntchito zake zimaphatikizapo zamankhwala ogulitsa, kuwunika kwachilengedwe, zolosera, ndi minda yambiri. Mu celmography imatcheru, ukhondo wa chindachi, makamaka mabotolo a cromagraphy, ndi ofunikira kwambiri. Zipangizozi ziyenera kutsukidwa bwino kuti zichotse zotsalira zomwe zingasokoneze kutsimikizika komanso kudalirika kwa zotsatira zowunikira. Kukhalapo kwa oyeretsa oyeretsa mu Mbale izi kumatha kupanga mitundu yosafunikira yomwe ingapangitse dece skew deta ndikuyambitsa zonena zolakwika.

Zotsalira

Zotsalira ZotsaliraMbale za chromatographyPambuyo poyeretsa kungathe kuphatikiza mitundu yosiyanasiyana. Alkaline oyeretsa monga sodium hydroxide nthawi zambiri amagwiritsidwa ntchito pochotsa zotsalira zachilengedwe, pomwe ma acidic monga hydrochloric acid atha kugwiritsidwa ntchito pochotsa ma michere. Ordol solt monga methanol ndi Ethanol amagwira ntchito posungunula omwe akuipitsidwa, komanso okonda kuchuluka monga triton X-100 nthawi zambiri amaphatikizidwa ndi zodetsa nkhawa. Zotsalira izi, ngati sizichotsedwa kwathunthu panthawi yopukutira, imatha kukhalabe pamtolo ndikuyanjana ndi zitsanzo pakuwunika kwa pambuyo pake.

Mukuyang'ana chitsogozo chokwanira pakuyeretsa mtunda wa chromatography? Onani nkhani iyi kuti mudziwe njira zokwanira kukonza njira zotsutsira bwino komanso machitidwe abwino:Zothandiza! Njira 5 zoyeretsa ma chromatography

Zokhudza Kuipitsidwa Kwambiri

Mphamvu yotsuka yoyeretsa yoyeretsa pa tepi imayipitsidwa. Ngakhale kuchuluka kwa mankhwalawa kumatha kuipitsa zitsanzo ndikusintha mawonekedwe ake. Mwachitsanzo, ogwiritsa ntchito amatha ADSORB pampandowo ndikukhuza machitidwe a owunikira, zomwe zimatsogolera ku chromatographic zotsatira zake. Kuphatikiza apo, otsalira otsalira kapena zoyambira zimatha kusintha pH ya zitsanzo, zomwe zimakhudza kununkhira kwa ionization ndi nthawi yosunga chromatographic. Sichilombo tomwe sichimangokhala ndi ziwonetsero za kuwunika kwa kuchuluka, komanso kumapangitsa kudalirika kwa chizindikiritso choyenerera.

Zotsatira pa Zochita Zoyeserera

Zotsalira zimatha kukhala ndi zotsatira mwachindunji komanso mwachindunji pa chromatographic zoyeserera. Chimodzimodzi mogwirizana ndi zolekanitsa ndi chromatographraphics zomwe zitha kuwonetsa monga kusintha kwa chithokomiro chovuta, phokoso lam'munsi, kapena kusunthika kosayembekezereka posunga nthawi yosungira. Kuphatikizidwaku kunganyozetsetsetsetsa chidwi, kuchepetsa chidwi, komanso kupewa kupezeka kwa mankhwala otsika. Kuphatikiza apo, kukhalapo kwa opezeka otsalira kumatha kupangitsa mankhwala kumachitika ndi kusanthula, kumapangitsa kuwonongeka ndi mapangidwe a kapangidwe kake, kusintha mawonekedwe a zitsanzo za zitsanzo.

Ndimachita chidwi posankha gawo labwino la HPLC yanu? Dziwani momwe mungasankhire yoyenera kuti ikhale yabwino kwambiri ya chromatographic:Kodi ndingasankhe bwanji kuyika kumanja kwa HPLC yanga?

Njira Yochepetsera

Kupanga njira zodzitchinjiriza ndikofunikira kuti muchepetse mphamvu zoyeretsa zoyeretsa pa cromophic kusanthula.

Kuyeretsa mokwanira:Khazikitsani njira yotsukira yoyenerera yomwe imalowa madzi mosalekeza ndi madzi oyeretsedwa kapena sol sol solven yoyenera kuchotsa zotsekera kwathunthu.

Protocols Protocols:Kukhazikitsa njira zotsimikizika zotsimikizika kuti zitsimikizike zotsika mtengo, mayeso ophatikizira dongosolo, ndipo zoyeserera zowongolera zimachitika kuti zitsimikizire kuti palibe amene akupuma.

Chida Chodzipereka:Ganizirani kugawa mabotolo kapena zida zoperekedwa kwa mitundu yapadera yopenda kuchepetsa chiopsezo cha kuipitsidwa.

Kuwongolera kwapadera:Gwiritsani ntchito njira zolimbikitsira zolimbitsa thupi monga makiyi a nthawi yayitali, jakisoni wopanda kanthu, ndi njira zodziwikiratu zowunikira, ndikugwiritsa ntchito zida zothandizira kukonzanso.

Oyeretsa oyeretsa mkatiMbale za chromatographyzimabweretsa zovuta kwambiri pakukhulupirika komanso kudalirika kwa chiwonetsero cha chromatographic. Mwa kukhazikitsa protocol yoyeretsa, njira zovomerezeka, ndi njira zoyenera, ma labotor amatha kuchepetsa mphamvu zotsuka ndi zolondola ndi zotsatira zowonjezera ndi zotsatira zazowonjezera za sayansi.

Kodi mukufuna kudziwa zambiri mwatsatanetsatane kapena kambiranani mutu wofanana?

Chidwi ndi mayankho pafupifupi 50 onena za HPLC Mbale? Lowani munkhani yokwanira ya mafunso anu onse okhudzana ndi mipata yambiri yamadzimadzi:50 mafunso ambiri omwe amafunsidwa kawiri pa HPLC Mbale

M'mbuyomu:

Tsogolo la kusefa

Ena:

Magulu a microscopic mu chromatography vials: zomwe zingachitike posungira zitsanzo zosungira ndi kusanthula