Ndezvipi zvisiri-zvisingaite mishonga yakaongororwa neGC-ms?

Gasi Chromatography-Mass SpectRogry (GC-MS) ndeyekuongororwa kwehunyanzvi hwekuongorora iyo inoshandiswa zvakanyanya kuongorora mashimoti echokwadi uye semivolele. Nekudaro, inogona kushandiswawo kuongorora maoni nonvolile kuburikidza nenzira dzakasiyana siyana, kusanganisira kuuyiswa. Chinyorwa ichi chinoongorora mhando dzevanonomisa mishonga inoongororwa neGC-MS, kukosha kwavo, uye nzira dzaishandiswa kuzviona.

Unoda kuziva zvakawanda nezve mutsauko pakati peLC-ms uye GC-MS, ndapota tarisa chinyorwa ichi:Ndeupi musiyano uripo pakati peLC-ms uye GC-MS?

Ndezvipi zvisina kunaka mishonga?

Zvisinganzwisisike macomputer zvinhu izvo zvisingaongorore nyore nyore pamhepo tembiricha. Ivo kazhinji vane huwandu hwepamusoro molecular huremu uye polarity, vachiita kuti vasiye vakakodzera kuongororwa zvakananga neGC-MS pasina kugadziridzwa. Mienzaniso yakajairika inosanganisira:

Polymers uye anowedzera: Zvinhu zvinoshandiswa mumapurasitiki uye marongero emidziyo.

Biobolecules: yakadai saAmino Acids, mapuroteni, uye mamwe maLipids.

Mishonga: inoshanda mishonga mishonga (APIS) uye metabolites yavo.

Pollutalental Pollutants: Enterly organic Pollutants (pops) uye simbi dzinorema.

Deivatization matekiniki

Kuongorora maoni nonvolile uchishandisa GC-MS, derivatization inowanzodiwa. Iyi process inosanganisira kumakemikari kugadzirisa nzvimbo yekuwedzera kuwanda kwayo kana kugadzikana. Nzira dzinowanzoitika dzinoitika dzinosanganisira:

Sielization: Kutsiva inoshanda hydrogen maatomu muboka rinoshanda neboka reSilicon (i.e., trimethysielsl). Iyi nzira inoshanda kune alcohols, Amines, uye Carboxyylic Acids.

Acylation: Iyi nzira inosuma mapoka e acyl kuti asimudzire kuutolility uye inowanzo shandiswa kune mafuta acids uye amino acids.

Methylation: Iyi nzira inowedzera methyl mapoka kune mapoka kuti awedzere kuwanda uye kuongorora.

Aya maThe Deatnizatizes maitiro anogona kushandura isiri-isina kujairika makomputa mune fomu iyo inogona kunyatsoongororwa neGC-MS.

Kuti uwane rumwe ruzivo nezve autosamupler vials yegesi chromatography, kureva chinyorwa ichi:2 ml autosamppler vials yegesi chromatography

Ndezvipi zvisiri-zvisingaite mishonga inogona GC-MS ishandiswe kuongorora?

1. Pointismal Pollutants

GC-ms inowanzojairisa kuongorora isiri-isina kujairika njodzi zvinhu zvakanyorwa nemasangano epamhepo. Semuenzaniso, iyo U.S. YEMAHARA YEMAHARA Dziviriro Agency (EPA) yakafunga nzira dzekuongorora pekutanga kusanzwisisika kwakadai se:

Polychlorinated biphenyls (PCBS): Chemaketi yeindasitiri anozivikanwa nekutsungirira kwayo kwezvakatipoteredza.

Mishonga yekuuraya zvipuka: zvasara kubva mukurapa maitiro anosvibisa ivhu nemvura.

Kuona miganhu yeaya makomputa kazhinji pakati pe1 kusvika 28 ppb, kuratidza iyo yakanyanya kunzwa kweGC-ms kana yakabatanidzwa neyakagadziriswa nzira dzakasarudzika

2. Chikafu Chekuchengetedza Kuongorora

Munzvimbo yekuchengetedza kuchengetedzwa kwekudya, GC-ms inoshandiswa kuona vasiri-vasingaenzaniswi zvinongedzo zvinogona kutama kubva pakurongedza zvinhu kuita zvekudya. Zvimisikidzwa izvi zvinosanganisira:

Plasticzeters: makemikari anowedzerwa kumafirlics kuti awedzere kushanduka; Mienzaniso inosanganisira PHTHALATES.

Kuwedzera: semuenzaniso, antioxidants kana anochengetedza anogona kusvetuka kuita chikafu.

Iko kugona kuongorora makomputa aya kwakakosha kuti uve nechokwadi chekuchengetedza kwevatengi uye kutevedzera zviyero zvekudzora.

3. Mishonga yemishonga

Ongororo yechechi inowanzoda kuzivikanwa kwe isiri-isina-mishonga mishonga nezvipikiso uye metabolites yavo. Mienzaniso inosanganisira:

Anoshanda mishonga yemishonga (API): Iyo yekutanga chinongedzo nekuda kwekurapa maitiro.

Metabolites: zvigadzirwa zvakaumbwa panguva yemetabolism yemushonga mukati megadziriro yezvinhu.

GC-MS inobvumira kuongororwa kwakadzama kweaya makomputa, kubatsira muPharmacokinetic zvidzidzo uye zvinodhaka zvigadzirwa.

4. Biological samples

Mune metabolomics, GC-ms inoshandiswa kuongorora isiri-isina-solatile metabolites mune yakaoma sampulogy sampulogs yakadai seurini kana ropa. Inowanzo kuongorora makomputa anosanganisira:

Amino Acids: Kuvaka mabhuru emapuroteni, ayo anogona kuratidza hutano husina hutano kana metabolic disorders.

Organic acids: Metabolites inobatanidzwa mune dzakasiyana siyana biochemical nzira.

Ichi chishandiso chinonzwisisika kunzwisisa metabolic masaini muchimiro chehutano nechirwere.

GC-ms ongororo nzira

Kuenzanisira Kugadzirira

Kana uchiongorora zvisingaenzaniswi zvisingaite uchishandisa GC-MS, inogadzirira yekuenzanisira inokosha. Maitiro anogona kusanganisira:

Liquid-mvura yekubvisa (LLLE): inopatsanura analys kubva pamatanho matrices.

Solid phase Exction (SPE): Inoisa Ongorora Inowanikwa Misanganiswa Yakaomarara isati yamuka.

Chishandiso

Yakanga yakajairika GC-MS Setup inosanganisira:

Gasi chromatograph: inoparadzanisa misodzi yezvikamu zvakavakirwa pane yavo yekuparadzira pakati pekumira uye nhare gasi gasi.

Massent SpectRrometer: inozivisa makomputa zvichibva pane yavo masasi-kune-kubhadharisa ratio (m \ / z), kupa ruzivo rwechimiro.

Kuongororwa kwedata

Kamwe chete misa misa inowanikwa, kuongororwa kwedata kunosanganisira kuenzanisa misa misa kune iyo raibhurari inozivikanwa kana database yekuzivisa zvakanyatsonaka. Advanced Software Zvishandiso Zvinofambisa ichi kuenzanisa, nokudaro kuwedzeredza kuzivikanwa.

Iwe unoziva mutsauko pakati peHPLC vials uye gc vials? Tarisa chinyorwa ichi:Ndeupi musiyano uripo pakati peHPLC vials uye gc vials?

Mhedziso

Gasi Chromatography-Mass SpectRometry inosara kiyi tekinoroji mukuongororwa kweinonocile makomputa munzvimbo dzakasiyana-siyana dzakadai senhau yezvakatipoteredza, kuchengetedzwa kwechikafu, mishonga yekudya, uye metabolomics. Nepo kuongororwa kwakananga kweaya makomputa kunetsa nekuda kwezvivakwa zvavo zvekunaka, nzira dzeruzivo dzakawedzera zvakanyanya chiyero cheGC-ms application. Sezvo nzira dzekuongorora dzinoramba dzichienda, GC-ms dzinogona kuita basa rinowedzera kukosha mukuita kuchengetedzwa uye kutevedzera maindasitiri paunenge uchifambira mberi mukutsvaga kwesainzi.