Chivabvu. 20, 2024
HPLC inyanzvi inobatsira imbudzi. Iyo inoiswa muminda yakawanda yesainzi. Pakati peizvi ndezve mishonga, kuongorora kwezvakatipoteredza, uye kuchengetedzwa kwekudya. Izvo zvakakosha kune maitiro ekuongororwa. Naizvozvo, kusarudza iyo chaiyo vial kwakakosha. Izvo zvinokanganisa chokwadi uye kuvimbika kwemigumisiro yacho.
HPLC vialsChengetedza samples dzako kubva pakusvibiswa uye nekunze pesvedzero. Iwe unogona kuwana idzi vials mumhando dzakawanda, saizi, uye zvigadzirwa. Ivo vanokodzera mhando dzakasiyana dzekuenzanisira uye kuongororwa zvinodiwa. Rudzi rwese rweHplc vial rundo inopa zvakanakira zvakasiyana. Ivo vane zvinhu zvinobatsira kuongororwa kwako kubudirira.
Hplc vials ine zvibodzwa zviviri zvikuru. Ivo vanochengeta samples yakachena nekumira kusvibiswa uye kugadzikana. Kusvibiswa kunogona kuita zvakasiyana siyana zvinokonzeresa. Semuenzaniso, zvinhu zvebhodhoro zvinogona kusevha. Iyo bhodhoro rinogona kudyidzana. Kana, vial vanogona kuve netsvimbo. Kugadzirisa idzi nyaya, vagadziri vanoshandisa girazi rakakwira-mhando. Iyo ine yakakwira boron zvemukati uye ndeyekugadzira HPLC samples vials. Vanosangana nenhamba dzehunyanzvi. Izvi zviyero zvinovhara iyo yemukati uye yekunze diamita yemuromo wemuromo nemuviri. Ivo zvakare vanofukidza kurongeka kwemuromo wakarukwa. Ivo vanosangana zvese zvinodiwa zvezvose zvinodiwa nedzimwe nyika. Imwe neimwe yechigadzirwa batch iri pasi peyakaomesesa mhando dzekutonga. Tine fekitori bvunzo report. Takaderedza njodzi yemuenzaniso kusvibiswa.
Chimwe chinhu chakakosha ndechekuti kana zvichienderana nemarara uye nhare mbozha mukuongorora. Zvinhu zvakasiyana-siyana zvevakasiyana vane akasiyana emakemikari kuramba kuita solies kana kupisa kupisa. Kusarudza izvo zvakaringana zvinhu zvinochengeta vial kubva pakuputsa. Izvo zvakare zvinodzivirira kukuvara kwekuenzanisa kana kusvibiswa.
Iyo yekuvhara system yeiyo vial inokosheswa kuti iite sampuli yakachengeteka. Septa anowanzogadzirwa kubva ptfe uye silicone, kudzivirira kubuda uye kuderedza njodzi yekusvibiswa. Chisimbiso chakanaka chinoita kuti sampuli yemuenzaniso. Iyi ndiyo kiyi panguva yekuongorora. Izvo zvinonyanya kufamba kwenguva refu kana mishonga yakaoma.
Sampuli vials zvinhu
Vazhinji vakunda vakunda vakagadzirwa negirazi. Iyo USP (United States Pharmacopepia) inokatyamadza girazi rekurambidza nekudzivisa kwemvura.
1. Nyora i, 33-yakawedzera borosilrate girazi
Borosilrate Girazi USP Typ Type 1, Giredhi A, 33 inert inter uye inoshandiswa zvakanyanya mumarabhoritari, kunyanya mu chromatography, uye ine silicon uye oksijeni. Iyo ine yakaderera resolution uye mutsara wekuwedzera kuwanda kuwanda kwe33.
2. Nyora i, 51-yakawedzera borosilrate girazi
Iyi girazi inonzi Borosilrate Girazi USP Typ Typ Typy 1, kirasi b, 51. Inogadzirwa zvakanyanya yeSilicon uye oksijeni. Iyo ine zvidiki zveBonon, sodium, uye zvimwe alkali simbi kupfuura kirasi girazi. Asi, inogona kuramba ichishandiswa kumarabhoritari. All Amber vials ndeyekirasi b vials ine coeff fiffery yekuwedzera kwe51.
3. PolyPropylene (PP)
PP ipurasitiki isingaiti. Iyo ine yakanaka yemakemikari kuramba. Izvo zvakakodzera kuchengetedza kwenguva pfupi yemakemikari mazhinji emakemikari. Inogona kushandiswa apo girazi haisi sarudzo. Kana uchishandisa anomatic kana halogenated hydrocarbons, kuramba kuderera nekufamba kwenguva.Pp vialsinoshandiswa zvakanyanya muIon chromatography. Izvi zvinodaro nekuti vane madiki ioni uye vanogona kucheneswa neisina simba acid uye madhiri emvura. Polypropylene vials chengetedza zvipfeko zvavo mumoto. Izvi zvinoderedza kuratidzwa kune zvinhu zvine njodzi. Iyo yakakwira yekushandisa tembiricha ndeye 135 ° C.
Kusarudzwa kwemuenzaniso vakurumbira kunogadzirwa kubva kune dzakasiyana zvinhu
a. Girazi - zvakasarudzika zvinoshanda uye zvakasarudzika;
b. Amber - yechiedza-chinonaka samples;
c. Polypropylene - kune zvinodhaka sampuli kana mvura-soluble solules;
Mhando dzeMuenzaniso Vals
Mafomu emienzaniso anowanzo kukamurwa mumhando nhatu. Izvi ndezvekuti: Crimp cap, screw mutsipa cap, uye snap mhete cap vials.
Ungasarudza sei pakati pematanho matatu ekuenzanisira?
Kune matatu marudzi emakapu anowanikwa kune viyeti viyeti: Crimp Caps, Snap Caps, uye Screw Caps. Imwe neimwe nzira yekuisa chisimbisa ine zvayo zvakanakira.
1. Crimp Pamusoro Cap
Iyo Crimp yepamusoro cap inotsvedza septum iri pakati pegirazi regirazi sampuli vial uye rakapetwa aluminium cap. Mhedzisiro inosimbisa yakanaka kwazvo uye inobudirira inodzivirira kubuda kwemuenzaniso. Septum inogara munzvimbo. Tsono inobaya sampuli. Kuisa MbiriCrimp cap vialsinoda kushandiswa kwemapapaper. Kune vashoma nhamba yemasampuli, iyo manyorero chivha ndiyo sarudzo yakanyanya kunaka. Kune yakakura huwandu hwemasampuli, iyo otomatiki yemukati inogona kushandiswa.
2. Screw mutsipa cap
The thescrew mutsipa capzvakasikwa. Kusimbisa iyo cap inotsvedza iyo spacer. Izvo zvinodaro nekushandisa simba rinozvimiririra pamusoro pegirazi rim uye aluminium cap. Munguva yemasampling, iyo screw cap inoisa nemazvo. Izvo zvakare zvine chekuita nePritum. Hapana zvishandiso zvinodikanwa kugungano. Iyo screw cap ine ptfe \ / silicone septum. Iyo yakabatanidzwa kune iyo polypropylene cap uchishandisa solvent-yemahara maitiro. Uku kusungwa tech kuchengeta septum uye cap pamwe chete panguva yekutumira. Zvinoita kuti vaite pamwe chete apo cap yakasungirirwa kune vial. Uku kusungwa kunodzivirira septum kubva pakusununguka panguva yekushandisa. Asi, iyo nzira huru iyo inosimbisa isimba kana iwe ukasimbisa cap mune vial. Iyo cap inonamatira yekuita chisimbiso. Iyo inobatawo septum munzvimbo panguva yekusagadzikana. Usasimbise iyo vial cap yakawandisa. Izvo zvichakuvadza chisimbiso uye kuti septum inowira pasi. Kana iyo cap yakasimbiswa yakanyanyisa, septum ichapukwa kapu kana kuti inyongwe.
3. Snap Mhete Cap
The thesnap capKuwedzerwa kwenzira yekuisa chisimbiso ye jaya chifukidziro. Iyo cap ipurasitiki. Inokodzera pamusoro peiyo rimu. Iyo inosimbisa nekukanda iyo septum iri pakati pegirazi uye iyo yakatambanudzwa purasitiki cap. Iko kushushikana mupurasitiki yekuvhara kunokonzerwa nekuedza kwayo kuti udzokere kuhukuru hwayo hwekutanga. Uku kusagadzikana kunogadzira chisimbiso pakati pegirazi, cap uye septum. Iyo plastiki snap cap inotsva pasina chero zvishandiso.
a. Mhedzisiro inosimbisa yeiyo snap cap haina kunaka sedzimwe nzira mbiri dzekuisa chisimbiso.
b. Kana iyo yakakodzera iyo cap yakasimba kwazvo, iyo cap ichave yakaoma kuvhara uye inogona kutyora.
c. Kana zvasununguka zvakanyanya, chisimbiso hachizoshanda uye septum inogona kubuda munzvimbo.
Izvo zvigadziko zvigadziko zviri nani pane bhodhoro. Rubber kana Silicone inosimbisa-chisimbiso mushure mekurohwa. Ivo vakanakira kuchengetedza reagents.
Nekudaro, mamwe mashoma ekunyora anogadzirisa Silica gel kana rabha gasi panguva yekushandisa. Mune ino kesi, vials inoda Septa ine ptfe coating. Kana panguva yekushandisa, iyo Reagent haina kushandiswa pakarepo, asi chikamu cheichasara mumuenzaniso muenzanisi wenguva inotevera. Kana iwe ukashandisa sampuli zvakare, izvi zvinoitika nekuda kwekupiwa kweSepta kwePTFE-yakafukidzwa Vial kurasikirwa Chisimbiso chavo.
Rubber kana silicone vial septas inodzivirira kune zvimwe zvinhu. Mabhodhoro akanaka ekuvachengeta mune avo vane crimp tops. Zvisinei, mamwe madzinza, mamwe marefu anokosheswa haafanire kusungwa mumabhodhoro akaomeswa. Rubbhi inoshanda zviri nani pane silicone maererano nekuiswa chisimbiso.
Muchidimbu, HPLC Reagent Mabhodhoro akakosha. Ivo vanochengeta samples vakachena uye vanove nechokwadi chechokwadi mhedzisiro. Kusarudza iyo chaiyo Reagent Type Type, saizi, uye zvinhu zvinogona kurongedza mhedzisiro yeHPLC yako kuongorora. Inogona zvakare kuderedza njodzi yekukanganisa. Iyo inogona kuve yakajeka girazi vial ye general ongororo kana amber imwe yerudzi rune mitsetse. Kana, inogona kunge iri chinhu chakakosha chevhu-diki-vhoriyamu kana pre-slit application. Iyo chaiyo HPLC vials inogona kubatsira chero basa rekuongorora kubudirira.
Chirungu
ChiChinese
