Ndeupi musiyano uripo pakati peGC-ms uye GC-MS \ / ms?

Gasi Chromatography-Misa SpectRogry (GC-MS) uye GAC-ms Nepo nzira mbiri idzi dzinoshandisa gasi chromatography (GC) yekuparadzaniswa uye misa spectrometry (ms) yekuzivisa, ivo vakasiyana kwazvo muchimiro chavo chekushandisa, kugona, uye mashandiro. Ichi chinyorwa chinoongorora misiyano iyi zvakadzama.

Chii chinonzi gc-ms?

Kuenzanisira Kugadzirira

Solid phase yekubvisa (spe) kana mvura-yemvura yekubvisa (lle) inowanzoshandiswa kubvisa matrix yekukanganisa uye kuwedzera kunzwa

Derivatization (i.e., methylation, trimethslilation) inogona kuvandudza kusungwa kwehupombi kana iyo thermy libile componeunds.

Mashandiro azvinoita

GC-MS inosanganisa gasi chromatography ine misa spectrometry yekuongorora kwemasanganiswa akaoma. Munguva iyi, sampuli inovhuvhurwa uye inotumirwa kuburikidza ne chromatographic column ichishandisa iyo inerer gasi seyenharisi chikamu chefoni. Kana ma compounds akapatsanurwa zvichibva pane yavo kujairika uye kudyidzana neyekutungamirwa chikamu, ivo vanounzwa mune misa misa spectrometer.

Zvikamu zveGC-MS

Gasi chromatograph: inoparadzanisa mishonga inosanganiswa musanganiswa yakavakirwa pane yavo yekubikira pini uye kushamwaridzana kwechikamu chenzvimbo.

Misa Mass SpectRrometer: Inoona uye inozivisa yakaparadzaniswa makomputa nekuyera iyo misa-kune-kubhadharisa ratio (m \ / z). Iyo inokonzeresa mashandiro anopa ruzivo nezve smecular uremu uye chimiro chevanoongorora.

Novel ionization zvinyorwa

Nyoro yeIonization hunyanzvi (i.e., APCI, DART) kuderedza kupatsanurana uye kusimudzira moleky ion signals.
Inotakurika GC-ms Systems ikozvino inoshandiswa pane-site isina njodzi kuongororwa uye kutarisa kwezvakatemerwa.

Zvikumbiro zveGC-MS

GC-MS ine akasiyana-siyana ekushandisa, kusanganisira:

Kuongorora kwechiitiko: Kuzivisa zvinodhaka, zvidhina, uye zvimwe zvinhu muzvisikwa zvebhaibheri.

Kuongorora zvakatipoteredza: Kuongorora zvinoshatisa mumhepo, mvura, uye ivhu.

Mishonga yemishonga: kudzora kwemhando uye maitiro ekuvandudza zvinodhaka.

Kuchengetedzeka kwechikafu: Kuona zvinoshatisa uye kuratidza chokwadi chechokwadi.

Indasitiri yepeturu: Kuumbwa kuongororwa kwekuvhunika uye kuyerera kwemafuta, kuwanda kwegesi-phase zvikamu.
Metabolomics: Offication uye Quicitative kuongororwa kwemadiki-madiki metabolites, anoshandisa huwandu hwehuwandu hwekuti awane biommarkers.

Chii chinonzi gc-ms \ / ms?

Mashandiro azvinoita

GC-MS \ / ms inosimudzira kugona kweiyo tsika yeGC-MS nekubatanidza tandem misa maspectrorry. Izvi zvinoreva kuti mushure mekutanga misa misa spectRometry kuongororwa (MS), iyo yakasarudzwa ion yakawedzera kupatsanurana mune yechipiri nhanho yeMisangano SpectRometry Ongororo (MS \ / ms). Iyi-nhanho-nhanho maitiro inogona kupa zvimwe zvakadzama ruzivo rwechimiro nezve fungidziro.

Zvikamu zveGC-MS \ / ms

Yekutanga Quadrupole (Q1): Mabasa kunge akajairwa misa spectrometer, achisarudza ion zvichienderana neye m \ / z ratio.

Celler Cell: Iyo inosarudzwa ioni yakasarudzika yakakamurwa necolision-yakakwenenzverwa dissociation (cid), inogadzira chigadzirwa ion.

Chechipiri Quadrupole (Q2): Chidimbu ioni anoongororwa kuti ape mumwe munhu akafungidzirwa uye kunzwa.

ION TAP \ / Yechitatu-Stage Tof: Mamwe GC-MS \ / Masisitimu inosanganisira chidimbu chioni kana chetatu-nhanho tof kuti ive yakadzika kurongeka.

Zvikumbiro zveGC-MS \ / ms

Iyo yakavanzika kunzwa uye yakatarwa yeGC-MS \ / gadzirisa yakakodzera:

Target Rehisation: Kuyera yakaderera-yakaderera yekuongorora chaiyo yekuongorora, iyo inokonzeresa kiriniki diagnostics.

Kuongorora kwakaomarara

Kuedzwa kwezvakatipoteredza: Kuona trace inosvibisa iyo inoda yakanyanya kunzwa.

Yakakwira-Kupfuura Pesticide Kuongorora: Uchishandisa Fast GC nzira uye akawanda maitiro ekugadzirisa (mm) kuona akawanda emishonga yekuuraya zvipuka panguva imwe chete.
Kudya kwekudya uye kutedzemerwa: Kuona mahomholoji uye magamba ezvivakwa kuburikidza nehunhu hwehukama.

Misoro yakakosha pakati peGC-MS uye GC-MS \ / ms

1. Kunzwa uye chokwadi

GC-MS: Inopa kuzivikanwa kwekutanga zvichienderana nekuchengetwa kwenguva uye misa misa, asi zvinogona kunge zvine manyawi nemusanganiswa wakaomarara umo makomputa akawanda compounds co-elute.

GC-MS \ / MS: Yakakwirira kunzwa nekuda kwekugona kuongorora chidimbu ion, kubvumira kuzivikanwa kwakanyanya kuzivikanwa kunyange mune yakaoma matrices. Izvi zvinoita kuti zvinonyanya kubatsira pakuona kuwanda-huwandu hwehuwandu.

2. Kuona muganho

GC-MS: Kuona miganhu inowanzo kuve yakakwira kuenzaniswa neGC-MS \ / ms. Inogona kuziva makomendi, asi arege kuvhiringidza zvakanyatsovanyatsovanza zvakanyanya.

GC-MS \ / MS: Yakavandudzwa Selection Kufamba Kwekudzokorora

3. Data kuoma

GC-MS: inoburitsa imwe misa misa yemugodhi kune imwe neimwe yakaonekwa yakakwanira, iyo yakakwana kune akawanda mashandisirwo asi anogona kusapa ruzivo rwakadzama ruzivo.

GC-MS \ / MS:

4. Kuomarara kuoma

GC-MS: Kazhinji yakareruka kushanda uye inosanganisira mashoma zvikamu; Inokodzera kuongororwa kwechiitiko kunoda kukwira kumusoro.

GC-MS \ / MS: Zvakaoma kuomarara nekuda kwekuwedzera kwezvikamu zvakadai seColiges masero uye akawanda quadrupoles; inoda kudzidziswa kwakasarudzika kwekushanda uye kududzirwa data.

5. Cost kukanganisa

GC-MS: kazhinji inodhura zvakanyanya mukutanga kwekutanga mari uye mari yekushandisa; Inokodzera mabara ane mabhajeti mashoma.

GC-MS \/MS: ine mutengo wepamusoro wekutanga nekuda kweTekinoroji yepamusoro uye yakawedzera zvinodiwa pakugadzirisa; Nekudaro, inopa mamwe masimba ekuongorora zvine simba anogona kururamisa iyo mari yekudyara kwemanyorero.

FAQ

Q: Ndeipi musiyano mukuru pakati peGC-ms uye GC-MS \ / ms?
A: GC-MS \ / Zvichida Kuwedzeredzwa Kunzwa uye Kujeka Nekuwedzera Chikamu chechipiri cheMisangano SpectRometry, Kubvumira Kuzivikanwa Kwazvo Kwemakomputa, kunyanya mumusanganiswa wakaoma.

Q: Ndirini pandinofanira kusarudza gc-ms pamusoro peGC-ms \ / ms?
A: GC-ms yakakodzera kuongorora kuongorora kweiyo isina kujairika makomisheni uko kwakanyanya kunzwa kusiri kutsoropodza. GC-ms \ / ms inosarudzwa yekuona kuongororwa kwakaderera-kuongororwa kwakawandisa mumatanho akaenzana.

Q: IS GC-MS uye GC-MS \ / MMS yakakodzera kune isina-isina kujairika mishonga?
A: Maitiro ese ari maviri anonyanya kugadzirwa kune asina kujairika uye akaomeswa makomputa. Isina-kusiririsa mishonga ingangoda derivatization kana dzimwe nzira dzakadai seLC-ms.

Q: Ko mari inoenzaniswa sei pakati peGC-MS uye GC-MS \ / ms?
A: GC-ms Systems kazhinji inowanzodhura uye ine mutengo wakaderera wekushandisa. GC-MS \ / Masisitimu inosanganisira yepamusoro yekutanga mari yekudyara uye kuchengetedza mari nekuda kwekugona kwavo kwepamberi.

Q: Ndedzipi mhando dzemakomisheni dzinogona kuongororwa?
A: GC-ms yakakodzera kuwiriraniswa kana hafu-isina kujairika organic makomposhi akadai sehahs, zvipuka zvezvipfava, vocs, uye mishonga yemishonga. Derivatization inowedzera chiyero charo kuti ipurasitire makomputa senge amino acids uye shuga.

Q: Sampuli inofanira kugadzirira sei GC-ms?
A: Kugadzirira kwekuenzanisa kazhinji kunosanganisira filtration, chipisa kana kutadza kubvisa matrix ekupindira. Derivatization (i.e., methyclation, sikulation) inodiwa polar kana inokanda mitsipa yemavara. Zvematanho akaomarara (i.e., ropa, ivhu), kucheneswa kwakawanda sileca silica gel column inokurudzirwa.

Q: Ndeipi yakajairika yekuona muganho weGC-MS?
A: Iyo yekuona muganho weGC-ms kazhinji mune yeNG-PG range, zvichienderana nekuita kwekuita uye kugadzirira. Ye porticide yakasara kuongororwa, inogona kusvika 1-10PG.

Q: Ndeipi iyo yakakwira molecular uremu gc-ms inogona kuongorora?
A: Nekuti sampuli inofanira kunge iri vapored, GC-ms kazhinji inoongorora mamorekuru kusvika kusvika 800da. Nezvipfuyo zvakakwirira uye zvinobva zvabviswa, izvi zvinogona kuwedzera ~ 1000da. Kune makuru mamorekuru, LC-MS inokurudzirwa.

Q: Ndinosarudza sei pakati peGC-MS uye GC-MS \ / ms?
A: Kana iyo chinangwa ongorora yekuongorora yakakwirira uye iyo matrix iri nyore, GC-ms yakakwana. Kune trace-level kuwanda kwematanho kana kuomarara matrices (i.ef.

Unoda kuziva zvakawanda nezve mutsauko pakati peLC-ms uye GC-MS, ndapota tarisa chinyorwa ichi:Ndeupi musiyano uripo pakati peLC-ms uye GC-MS?

Visual Elements \ / Kuenzanisa Overview Tafura

Iyi tafura inobatsira nekukurumidza kunzwisisa misiyano yakakosha pakati penzira mbiri idzi.

Mukupfupikisa, zvese gc-ms uye gc-ms maitiro ane imbe erugwaro anotamba chinzvimbo chakakosha muminda dzakasiyana siyana dzesainzi. Nepo GC-ms yakakodzera kuongororwa kwegore rehurongwa hwekuita, GC-MS \ / MS " Sarudzo pakati penzira idzi mbiri dzinoenderana nezvinodiwa zvekuongorora zviri kuitwa, kusanganisira zvinangwa zvekunzwa, kufunga kwechikepe, uye budgeary mazano ekushanda kwemasangano. Kunzwisisa misiyano iyi inobvumira vaongorori kuti vasarudze maitiro ayo anonyatsokodzera zvido zvavo zvekuongorora, kuona kuti zvavanowana ndezvechokwadi.