Kutora iyo chaiyo autosamppler vials kiyi. Uchishandisa zvakanaka ndezve, zvakare. Ivo vanosarudza kuti yakavimbika sei uye yakajeka HPLC ongorora ndeye. Iyo vhoriyamu yeVial inogona kukanganisa kutendeka kwemuenzaniso. Inobatawo chokwadi che data. Ichi chinyorwa chinoongorora nzira dzekudzivirira HPLC autosamppler vial vhoriyamu. Chinangwa ndechokucheka kurasikirwa kwekuenzanisira uye chengetedza kuongororwa kwechokwadi.

Kunzwisisa Zvakajairika Vhoriyamu

Pane huwandu hwakati wandei hweHPLC autosamupler zviripo, senge 1.5-2 ml, 4 ml, uye 150-300 μl micro insert. Iyo inonyanya kushandiswa uye inokwanisa kusangana nezvinodiwa pakuongororwa kwemazuva ese ndeye 1.5-2 ml samples vials. Kana samples dzinoda mavhambadziro mukuru kupfuura 3 mL, isu tinoshandisa yakakura 4 mL midziyo. Aya makomputa akakura anogona kubata micro erirts mukati mavo. Izvi zvinoshandiswa kune madiki madiki ekuenzanisira mavhoriyamu, pakati pe150 ne 300 μl.

Kuziva maitiro uye kushandiswa kwakakodzera kweaya HPLC autosamppler vial saizi kiyi. Kusarudza iyo chaiyo vial size ndeyekutsoropodza. Inofanira kuenderana nemuenzaniso wekuratidzira. Izvi zvinobatsira kuderedza sample kurasikirwa uye chengetedza iyo sampuli yekufungidzira panguva yekuongorora.

Kuda kuziva zvakawanda nezveHPLC vial inoisa, ndapota tarisa chinyorwa ichi HPLC Val Inserts: Kuvandudza ChaCecision uye Sampuli Kutendeka

Maitiro ekusarudza zvinoenderana nemuenzaniso

Kusarudza iyo yakakodzera vial saizi inokosha kuti ideredze kurasikirwa kwemuenzaniso. Semutongi mukuru, iyo sampula vhoriyamu inofanira kunyatsoenderana neiyo vial kugona. Uchishandisa vial iyo yakakura kwazvo kuti muenzaniso unogona kugadzira zvakanyanyisa musoro. Izvi zvinowedzera njodzi yekubuda, kunyanya kune kuongororwa kusiri. Zvekare, kusarudza vial iyo idiki kwazvo kunogona kutungamira kumienzaniso kufashukira uye kurasikirwa.

1.setsana Muenzaniso vhoriyamu:

Kuyera kana kufungidzira iyo vhoriyamu yeiyo HPLC yako sampuli.

Funga zvinotarisirwa vhoriyamu shanduko. Shanduko idzi dzinogona kunge dzakonzerwa nekudhura, kugadzirisazve, kana mamwe matanho ekugadzirira.

2.match iyo sampuli vhesi kune yakajairika vial saizi:

Zvemhando diki yekuenzanisira (150-300 μL), micro mainesmi akaiswa mukati meiyo yakakura vial (i.e., 2 ml) inokurudzirwa.

Yepamhepo yemuenzaniso voluma (0.5-2 ml), standard 1.5-2 ml vials kazhinji sarudzo yakanyanya kunaka.

Kune yakakura sampula mavhoriyamu (kusvika 3 mL), 4 ml viums inogona kunge yakakodzera kwazvo.

3.consnder the mumusoro:

Chinangwa kuderedza misoro (isina vhoriyamu) mukati meiyo vial.

Kunyanyisa mumusoro kunogona kuwedzera njodzi yemuenzaniso wemuenzaniso, kunyanya kune vanoongorora kuongorora.

Iyo sampuli vhoriyamu inofanira kunge iri padyo neiyo vial septum kana cap yekugadzira chisimbiso chakasimba.

Nyaradzo inodiwa yekuisa:

Kune madiki madiki mavhoriyamu, iyo micro isa mune yakakura vial inogona kuderedza musoro. Iyi seti zvakare inoderedza kuratidzwa kwemuenzaniso.

Micro insert inouya mumavhoriyamu akasiyana-siyana, senge 150-300 μl. Ivo vanogona kukwana akasiyana saizi saizi.

Kuda kuziva nzira yekusarudza iyo chaiyo mhando ye micro-insert yeyako chromatography vials Maitiro ekusarudza iyo chaiyo mhando ye micro-insert yeyako chromatography vials

5..test uye inosimbisa:

Mufambiro Wekutanga kuyedza uchishandisa iyo yakasarudzwa vial saizi uye chero mainorts.

Tarisa uone kupora kwemuenzaniso, kugadzikana, uye chero zviratidzo zvekuveza kana kusvibiswa.

Gadzirisa saizi yeVial. Kana, shandisa mainorts sezvinodiwa. Izvi zvichawedzera sampuli yekuenzanisira uye kuchengetedza.

Tevedza matanho aya. Iwe unogona kuwana iyo yakanakisa HPLC vial vial saizi yeiyo yako sampuli vhoriyamu. Izvo zvakare zvichabatsira kuderedza kurasikirwa kwemuviri. Rangarira kufunga nezve sampu yese yekutanga. Zvakare, funga nezve chero chinhu chakakosha kuongorora kana iwe ukasarudza.

Kuda kuziva nzira yekusarudza iyo chaiyo cap ye chromatography yako vial, ndapota tarisa ichi chinyorwa Maitiro ekusarudza iyo chaiyo mhando yeInserts yeChromatography yako vials

Kuderedza misoro uye kuratidzwa

Chinhu chinonyanya kukosha ndechekugona kusarudza vial vhoriyamu iyo yakanyanya kukodzera nhamba yemasampuli. Izvi zvinobatsira kuderedza misoro uye kuderedza nzvimbo yepasi pachena kune zvakatenderedza nharaunda. Nekunyatsofananidza vial vial saizi kune sampuli vhoriyamu, iwe unogona kunyatso kuderedza kurasikirwa kwemuviri nekuda kwekuveura kana kupfira.

Kana iyo semuenzaniso huwandu idiki uye yakakura vial inoshandiswa, zvakawandisa zvemusoro kana vhoriyamu isina chinhu ichagadzirwa mune iyo vial, iyo ichakurudzira sampula yekubuda uye kukanganisa kutendeka kwemuenzaniso. Kuti uderedze njodzi iyi, saizi diki diki iyo inogona kugamuchira iyo sampuli vhoriyamu ichiri kubvumira kuiswa chisimbiso uye kubata kunofanirwa kushandiswa. Kuverengera kuti sampuli iri kutaurirana zvakananga neiyo vial septum kana cap inobatsira kugadzira chisimbiso chakasimba uye kuderedza nzvimbo yakafumurwa.

Kusimbisa vial inserts

Vial Insert, kunyanya micro enjodzi, inogona kupa imwe denderedzwa rekudzivirira kwezvidimbu zvidiki zvehasi. Izvi zvinopfekera zvinopindirana mukati mehukuru hwemuvhu, zvinodzora zvinobudirira misoro iripo uye kuderedza kuratidzwa kwemuenzaniso. Semuenzaniso, iwe unogona kuve nechokwadi chekuvimbika kwemuenzaniso madiki ekuenzanisira nekuisa inopinza kupinda mu 1.5-2 ml vials.

Mhedziso

Kusarudza iyo yakakodzera HPLC autosamppler vial vhoriyamu yakakosha kuchengetedza kutendeka kwekuongorora kwako kwerudzi. Munyaya ino, takadzidza nezvezvakawanda zvemavhoriyamu, kuti vangasarudza sei vial, uye nei vakakosha. Isu tichasarudza iyo yakakodzera vial kuti ideredze kurasikirwa kwemuviri uye kunopa yakavimbika data.

THELVE muzvishandiso zvakasiyana zve chromatography vials mune chikafu uye chinwiwa chekupedzisira, kuongorora kwavo kwakakosha basa mukuita kuchengetedzwa uye hunhu hwemhando:HPLC yemuenzaniso yekugadzirira mhinduro dzemhedzisiro yakanaka

Yapfuura:

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