Kufungidzira kwekunyepedzera kunosanganisira zvese mashandiro pamberi peku jekiseni. Kunze kwekurema, kupera simba, uye kusvibisa, zvinosanganisirawo matanho anotevera:

Filt
Kubviswa
Derivatization (pre-column derivatization)
Liquid chromatography (low-Pressure column chromatography)

Kukurudzirisa kunogona kuitwa mumanzwiro kana otomatiki. Iwe unoziva here nezve sampuke ekunyepedzera?

Derivatization

Mamwe masampula haakwanisi kuremerwa zvakananga mushure mekunyepedzera. Derivatization inodikanwa kuti iite makomputa pasina uv yekunyudza kana fluorescence inoonekwa neUV kana Fluorescence Detectors.

Inovandudza pfungwa
Inowedzera kupatsanurwa (shanduko yemhando)

Nyaya dzingangoitika dzinosanganisira kurasika kwemuviri, kusvibiswa, kusagadzikana, kuita kwakawanda ne-zvigadzirwa, izvo zvinogona kukanganisa kurongeka kana kuunza zvikanganiso.

SAMPLE FILTRAL

Lc samples dzinofanira kunge dzakazara vunifomu uye tikamu. zvidimbu zvinogona kukuvadza iyo jekiseni kana kuvhara iyo chikamu. Matanho anosanganisira:

Zunza sampula usati jekiseni uye tarisa zvimedu, turbidity, kana emulsions
Kana zvimedu zvichicherechedzwa, firisa sampu uchishandisa membrane iyo inogona kuchengetedza ≥0.15QM zvimedu

Zvingangoitika Nyaya: Sampula Kusvibiswa, Adsorption Kuderedza Component Constentration, uye Solventever East inokonzeresa zvikanganiso.

Nzira Dzinowedzera

Kuwedzera kuparadzanisa kuongorora kubva kuCo-sol solves kana kunobvisa kupindira zvinhu (i.e., mapuroteni). Organic sol solile inofanira kunge yakaderera-yehupfumi, isina kujairika, yakaderera mukusviba, uye inovhara muenzaniso wakanaka.

Zvakajairika sol solvent: diethyl ether, ethyl acetate, dichlommethane, chloroform, benzene, kana musanganiswa.

Mushure mekurwiswa, samples inogona kuiswa zvakananga kana kuomeswa kuti uwedzere kuongorora kuongorora uye kunzwa.

Mvura-Yakadzika Sample Exction

Acidic zvikamu uye salts: Bvisa kusvibiswa zvine simba solvent, chinja kune acidic, wozobvisa kana kujekesa; kana yakaoma pasi pe n₂ uye nyungudika mune yakakodzera solvent usati jekiseni.
Zvikamu zvakakosha uye salts: Bvisa kusvibiswa ne organic solvent, gadzirisa kune Basic, wozobvisa kana kujekesa; kana yakaoma pasi pe n₂ uye nyungudika mune yakakodzera solvent usati jekiseni.
Zvepfungwa Nezvekusaruramisa: Bvisa Kusvibiswa kweiyo organic solvent, wozoongorora zvakananga nekudzoreredzwa-phose chromatography.

Mafuta-soluble sample Exction

Bvisa neiyo organic solvent kana jekise zvakananga; kana yakaoma pasi pe n₂ uye nyungudika mune yakakodzera solvent usati jekiseni.

Kusvibisa masosi

Zvingangoita zvinyorwa zvekusvibiswa mukuongorora:

Kukanganisa rudzi	Kunobva	Kudzivirira Matanho
Zvakatipoteredza	Magasi anokuvadza, guruva, erosiols	Chengetedza imba yemusango, yakachena; Isa ac; Dzora humidity <70%; Dzivirira unyoro, Corrosion, vibration
Midziyo	Girazi, Ceramic, Quartz, Plastiki Labware	Sarudza midziyo yakakodzera pane sampuli mhando; kuchenesa zvakakwana
Reagents	Non-chromatography-giredhi reagents	Shandisa chromatography-giredhi, yakakwirira-yekuchena, kana ongororo-giredhi reagents

Standard Solution Kugadzirira

Gadzirira mhinduro yemukati kutanga; shandisa chibharo chimwe chete mune zviyero uye samples kuderedza zvikanganiso
Zvinhu zvinofanirwa kunge zviri chromatography-giredhi uye yakagadzikana
Chengetedza zviyero zvakajairika mumabhodhoro ebhuruu pakudziya kwakadzika
YeVitamins, shandisa brown volumetric flasks kana chengetedza kubva pachiedza

Matambudziko akajairika mune sampuke ekunyepedzera uye mhinduro

Dambudziko	Chikonzero	Mhinduro
Isina kuwirirana Peaks mu chromatogram	Filter Kusvibiswa	Soak firita mune sample solvent uye bvunzo jekiseni Shandura Filter Type Shandisa imwe nzira yekuchenesa nzira
Yakadzika Peak Mhinduro (kunyanya yakaderera-kusimbisa samples)	Adsorption pane firita pamusoro	Shandura Filter Type Maitiro ese samples mumamiriro akafanana Shandisa imwe nzira yekuchenesa nzira
Kupora kwakaderera	Isina kukwana kubviswa	Wedzera nguva yekubvisa, shandisa kupisa solvent Rongedza nzira yekuchenesa
Yakakura Peaks, yakapfupikiswa chikamu cheLumende	Sampu inopindira uye kusvibiswa	Kuvandudza nzira yekuchenesa
Kurongeka kwakaringana	Kupora kusakwana	Simbisa kana kutsiva derivatization, kupatsanurwa, kubviswa, kana mamwe mamiriro Shandisa otomatiki kubata kuti uvandudze precision

Yapfuura:

Yakanyanya kukwirira \ / yakadzika tembiricha uye yakajeka yekufumisa madhara pane sampuli kugadzikana: fungidziro & madzinza

Kudzokera kune runyorwa