Low-adsorption vigials, vial nzvimbo yekurapa, sielized vials, peg yakachekwa vials, PFDCs Coating

Mukukwira-sensitivity kuongorora, adsorption kurasikirwa pane vial nzvimbo kunogona kudzikisira chokwadi. Intrinsic silanol mapoka (si-oh) uye trace simbi yekutsamwa mugirazi inogadzira hydrogen zvisungo kana magetsi ekusangana nemamolekhushi nemamolecules, gayo polar kana kubhadharwa madziro emadziro. Vasingazivikanwe Borosilrate vials vanowanzoburitsa polar drug kana biomelcule vanodzoreredza pazasi 80%, uye otomatiki sampling vashoma chiratidzo chekushora kwakadzokororwa. Vatengesi vanokurudzira sielied girazi vials yePolar Ongorora kuongorora negirazi kuongorora Naizvozvo, pamusoro pekupaza kana kuunganidzira kune mhosva yekuteedzera-mwero.

2. Girazi rinoshanda sites uye adsorption michina

a. Silanol mapoka uye simbi ion
  Ini. Pamusoro si-oh mapoka anosunga polar ongororo isingadzoreki
  Ii. Trace simbi ion inoumba electrostatic yekudyidzana neakabhadharwa mamorekuru

b. Solvent kuvhunduka
  Ini. Organic solventes (i., Acn, meoh) inogona kushatisa mapepa ekupaza, azivisa masaiti idzva anoshanda

c. Kupararira Kusvibiswa
  Ini. Vakasara vanobhadharwa kana hydrophilic mamorekuru pamadziro vanogadzira ghost peaks mune inotevera kumhanya

d. Otomatiki system mhedzisiro
  Ini. Inodzokororwa majekiseni mune yakakwira-yekupfuura system inowedzera kuteya kwe polar kana trace ongorora
  Ii. Yakashuma chiratidzo kurasikirwa kazhinji inopfuura 10% nekufamba kwenguva

3. Mhepo yekurapa nheyo: deactivation vs. coating

3.1 Dhizimusi rekutyaira

a. Yakakwira-tembiricha ichipfura (~ 800 ° C)
  Ini. Anomukira imwe si-oh asi inosiya simbi ion intact

b. Acid ged (i.e., 6 m hcl)
  Ini. Inobvisa mrist ions asi ma rougens girazi

c. Base wash (i.e., 1 m naoh)
  Ini. Inogadzira imwe-o⁻ Sites, inopindirana

d. Zvisingakwanisi
  Ini. Kungodzikisira chete kwezvinhu zvinoshanda pane girazi substrate

3.2 silanation

a. Organiilan kurapwa pasi pePuum
  Ini. OrgOilanes (i.e., methylilene) fomu covalent si-o-si bonds ine pasi silanols
  Ii. Inogadzira hydrophobic chipingamupinyi icho chinoramba kupisa, acids, uye zvigadziko
  III. Inokwereta pamusoro pekunetsana uye inodzoreredza polar analyina kudzoreredza kupora kusvika 90%

b. Vendor Mienzaniso
  Ini. "DV" Sielized vials for for polar-compound ongororo (mvura)

3.3 Zvekuita Coatings

a. Perfluorodecyltrichlosolosiyene (PFDCS)
  Ini. Kuzvisunga-kusunganidzwa monolayer inopa superydrophobic pamusoro
  Ii. Yakanakira kune isiri yepasitora pahs uye lipid-slullable inosvibiswa

b. Polyethyle Glycol (Peg)
  Ini. Mahyritani echeni inodzosera mapuroteni, mapepeti, uye mvura-soluble analyte
  Ii. Inopa dziviriro yepamusoro ye biobolecules

4. Adsorption Kudzora michina uye data

a. Pfungwa dzePassivation
  Ini. Silane Laers inopa girazi hydrophobic, kuvharidzira polar kusunga
  Ii. Yakagadzikana mushure mekuwedzera kunyudzwa mu acn kana meoh

b. Kupora kuita
  Ini. Sielized vials vanochengeta pedyo-100% kupora kwe1 ppb doxepin nekufamba kwenguva
  Ii. Peg-cated vials vanowana 97-99% kudzoreredzwa kwePolar
  III. PFDCs vials inodarika 90% kupora kwePAH iyo yakafananidzwa neyakaderera tsika pane girazi risina chinhu

c. Hama yekushambadzira
  Ini. Polar Angtes: Peg> Sielized 【PFDCS> Deactivated
  Ii. Nonpolar Ongorora: PFDCS> Sielized> Deactivated> Peg

5. Kusarudzwa kwekushandisa uye maitiro akanakisa

a. Enzanisa kurapwa kune sampu yemakemikari
  Ini. Polar Compounds (zvinodhaka, mapuroteni, makaruru, shandisa sielized kana peg coatings
  Ii. Nyowani dzisiri dzepasi
  III. Samples dzakasanganiswa: Silanization inopa kuita kwakaringana

b. Funga solvent uye nharaunda
  Ini. Silane Coatings shasha ph 1-12 uye yakawanda organics
  Ii. Polymer zvipfudzo zvinogona kusvibisa pasi pehusiku hwakasimba kana kupisa kwakanyanya; funga nezvePTFE inoisa kana polypropylene viums yemamiriro ezvinhu akanyanya

c. Sampuli vhoriyamu uye jekiseni mapoka
  Ini. Yemicrovovolumes (<100 μL) kana kudzokorora sampling, shandisa midziyo yekubhadhara
  Ii. Tarisa kuunganidza wakatendeka kuburikidza neKunonoka ANLLE (>>>>>>>>>

d. Bhajeti inopesana nekushandiswa
  Ini. Deactivation: Yakaderera mutengo, yakakodzera kudzidzisa kana kuwanda mitezo
  Ii. Sielized vials: Mid-Range mutengo, yakafara HPLC \ / LC-MS application
  III. PEG \ / PFDCS Coatings: Premium Mutengo, Yakanakira Bioanaly Bioanalyses uye Trace Yekutarisa Kwezvenzvimbo

6. Mhedziso: Kubva pane chombo chichava kuenda kune inoshanda interface

Sezvo auyelytical sensitivity inosvika PPB \ / ppt mazinga, viumple vials vanova vanoshanda zvinoshanda pane kungoita midziyo. Targeted kurapa kwakaderera-kushambadzira kunoshandura kurasikirwa kusingafungidziriki kuri kuita marapita ekudzora. Vals Sarudzo uye kurapwa kwepasi kwakakosha zvinhu zvakadzika-mwero-huwandu hwehuwandu. Nekufananidza tekinoroji yekubatanidza kune sampulies chemistry, maredhiyo anotendeuka mumasodhi ekushandisa, achivandudza kwazvo kurongeka uye kubereka mukuongorora kwekuongorora.

Zviitiko zvakakosha

1. Yeiyo Ultra-inotarisisa ongororo, shandisa mapundu kana akabatanidzwa vials

2. Mechi yePolarity: Sielized \ / Peg ye hydrophilic, PFDCs yeHydrophobic

3. Monitor kuunganidza: Chengetedza nzvimbo dzakachena, track yekubata angles, mhanya mabara, kutsiva pakutadza

4. Balance mutengo vs. data mhando: Preemium Coatings kuderedza reRures uye Nhema Nevatano