Chromatography is a qualuable auyelytical maitiro anoshandiswa mune dzakasiyana siyana dzesainzi kuronga kwakadai semakemikari, biochemistry, uye sainzi yemunharaunda. Iyo inoita basa rakakosha mukuparadzanisa kunze, zvichiratidza, uye zvikamu zvakakosha mukati memusanganiswa; chimwe chinhu chinokanganisa mhedzisiro yayo yekuongorora inogona kuve kwayo vial vhoriyamu yaishandiswa kubata samples; Mune chino chinyorwa chatichaongorora chinhu ichi chakakosha kuitisa chromatography kuongorora.

Kunzwisisa Chromatography

Chromatography ishizha rekurambidza rekuparadzanisa uye kuongorora musanganiswa. Inoshandisa zvikamu zviviri, iyo nhare chikamu (iyo inogona kunge iri yemvura kana gasi) uye stationary chikamu (kazhinji chakasimba kana chinwiwa), kuongorora chero musanganiswa uchidzidzwa. Kana ikasanganiswa muhurongwa, zvikamu zvakasiyana ita zvine stationary chikamu uye nhare chikamu zvakasiyana uye kufamba pamitero yakadzika zvichitungamira kuparadzaniswa.

Vial vhoriyamu uye chromatography kuongorora

Vials inoshandiswa muChromatographic ongororo dzinogona kuve nepfungwa huru pane mhinduro. Mumwe paramende akaremara kuti arambe achifunga kana achitarisa mumuenzaniso wekusanganavial vhoriyamu. Heino maitiro aungano kukanganisa kuongorora kwemuenzaniso:

SAMPLUL SIMBA:Kuwedzera kweVial vhoriyamu kunoita kuti sampuli inokonzera samples dzakaenzana nekusagadzikana zvishoma nezvishoma, zvichitungamira kukurudziro yemuenzaniso seyezinga dzevasungwa dzinoderera mu vial. Kana zvakananga zviyero zvakakakosha kuti uongororwe, danda rakadaro rinogona kupokana uye kushandura zvinoguma.

Kuona miganhu:Chromatography kuongororwa kunoda chaizvo chiitiko chekuona kana uchibata nehuremu ingaongorora kana kuderera samples, senge tracer kuongorora kana kuderera samples. Chikamu chidiki vhoriyamu chinogona kubatsira kuzadzisa zvakaderera kutarisa miganhu sezvo kusunganidzwa kwemasampuli kuchave kwakakura kana zvirimo munzvimbo diki.

Kunzwa:Chromatographic nzira dzinowanzoenderana nekunzwa kwavo nekuongorora kwekuongorora; Midiki madiki mavhoriyamu anogona kazhinji kazhinji anowedzera kunzwa uku, zvichiita kuti zvive nyore kuona uye kuti zviwedzere kuderera-makomputa.

Solvent Shandisa:Kushandisa madiki madiki mavhoriyamu anoda kushoma soli yekuongorora, iyo inogona kudzvanya mari uye uve wakanaka kune zvakatipoteredza.

Matrix Mhedzisiro:Zvemasampuli akaomarara, uchishandisa madiki madiki mavhoriyamu anogona kubatsira kudzika matrix matrix mhedzisiro. Matrix maitiro anoitika kana mamwe makomendi mune sampuli matsrix inokanganisa nekuongororwa kwekuongororwa kwakanangidzirwa analyte; Nekuderedza iyi vhoriyamu mhedzisiro yakaderedzwa pane izvi zvinopindira makomisheni.

Kuda kuwana mhinduro dzakaringana nezve HPLC vials? Mira mune ino nyaya yekunzwisiswa-kwakadzika:50 Mibvunzo inowanzo bvunzwa paHPLC vials

Mhedzisiro pane Peak chimiro uye resolution

Kubatana pakati pekusimbisa solvent uye nhare mbozha inoita basa rakakosha mukusarudza peak chimiro. Paunenge uchishandisa sampuli solies ine simba rekusimbisa kupfuura iyo foni chikamu, mapepa angaratidzika akawedzera nekuda kwekukurumidza-pane-anotarisirwa kusvika-kutarisirwa kwekuongorora. Izvi zvinokanganisa zvinogona kuwedzera kuwanda kwehuwandu hwekujekesa, zvichikonzera kusagadziriswa kusiririsa uye kushoma mapundu anopinza.

Kukurukurirana, uchishandisa sampuli solile ine simba rekusimbisa simba rinogona kuvandudza peak chimiro nekudzvinyirira kusimba mukati mekisimusi, inotungamira kupatsanurwa kuri nani kwekuongorora. Ichi chimiro chinosimbisa kukosha kwete chete yeiyo vial kugona asiwo sarudzo yekugadzirisa solvent inoshandiswa pamwe chete nayo.

Zvinokosha Zvinofungidzirwa

Zvidikivial vhoriyamuinogona kupa huru kunzwa kukuru uye kupindira kupindira; Nekudaro, kufunga kunoshanda kunofanirawo kufanirwa. Muenzaniso weSampuli unofanirwa kusangana nezvinodiwa zvechifunda; Mavhoriyamu madiki anogona kunge asiri kugara akakodzera.

Dzimwe nguva vatsvagiri vanosarudza kusarura masampula avo vasati vaongorora, vachivigira kuti vashandise madiki madiki madiki pasina kukanganisa kutendeka kwemuenzaniso.

Mhedziso

Vial vhoriyamu yakakosha kufunga mu chromatography kuongorora uye kunofanirwa kusarudzwa zvakanyatsosarudzwa kuti uwane mhedzisiro chaiyo uye yakavimbika. Vanotsvagurudza uye vanoongorora vanofanira kusarudza chakakodzera vial vial vhoriyamu vanoziva - mamiriro ezvinhu akadai sekuona miganhu, etc.

Kutarisa kuongororwazve? Mira mune ichi chinyorwa pane 15 application of Chromatography vium:15 zvikumbiro zve chromatography vials muminda dzakasiyana

Yapfuura:

Zvikumbiro zve chromatography vials mumafuta negesi kuongorora

Ini ndinosarudza sei iyo yekupa kurudyi kweHPLC yangu?