Oct. 18, 2024
Gasi chromatography (GC)Iyi yakakosha imbudzi yekuongorora inoshandiswa kupatsanura uye kuongorora makomputa mumusanganiswa. Imwe yeakakosha zviyero muGC iri nzvimbo yepamusoro, iyo ine chekuita nekuvharirwa kwekuongororwa mune sampu. Kunzwisisa zvinhu zvinokanganisa nzvimbo yepamusoro yenzvimbo yakakosha kuwana yakavimbika uye inobereka mibairo. Chinyorwa ichi chinotarisa zvakadzama pazvinhu zvakasiyana siyana zvinokanganisa nzvimbo yeGC Peak, inosanganisira jekiseni maitiro, mamiriro emukati, fanical mamiriro, uye maitiro ekuenzanisira.
1. Injection maitiro
Nzira yekupusa jekiseni inoita basa rakakosha mukusarudza nzvimbo yepamusoro:
Ingined vhoriyamu: huwandu hwemuenzaniso hwakagadzirwa hunofanirwa kuve hwakajeka. Kukurira iyo ikholamu inogona kukonzera kukanganisa kwepamusoro sekumberi kana kuswera, iyo inogona kukanganisa nzvimbo yakayerwa. Kukurukurirana, isina kukwana jekiseni vhoriyamu inogona kuguma neyakaderera chiratidzo uye yakaderedzwa nzvimbo yePeak.
Inomhanyisa jekiseni: Iko kumhanya uko iyo sampuli inogadzirwa inogona zvakare kubata Peak chimiro uye peak nzvimbo. Kukurumidza majekiri kunogona kukonzera nyonganiso, inotungamira kusanganisa kusanganisa uye band inokura, pakupedzisira kuderedza nzvimbo yepamusoro.
Injection mode: inopatsanurwa majekiseni anowanzo shandiswa kuongororwa kwekutevera nekuti ivo vanobvumira kuwedzera ana ana analyte kuti vapinde muchikamu, chinogona kuwedzera nzvimbo yepamusoro. Mukupesana, kupatsanura majekiseni kukosora sampuli, zvichikonzera munzvimbo diki dzepamusoro kuti dzive dzakanyanyisa samples.
2. Column mamiriro
Hunhu hwekamuri ine chekuita zvakanyanya munzvimbo yepamusoro:
Column mhando uye saizi: sarudzo yekoramu (i.e., capillary vs. yakazara) inokanganisa kugadzirisa uye kushanda zvakanaka. Capillary makoramu anowanzove ane madiki madiki uye akakura nzvimbo nzvimbo, zvichikonzera kupatsanurana zviri nani uye zvakapatsanura mapundu.
Column tembiricha: tembiricha kudzora inokosheswa muGC. Tembiricha yepamusoro dzinogona kusimudzira kusanyadziswa uye kugadzirisa kusarudzika, asi zvinogona zvakare kukonzera mapundu akakura kana isina kubatwa nemazvo. Kukurukurirana, kupisa kwakadzika kunogona kuguma nekuzvidzora kwenguva refu uye kuwedzera nzvimbo dzepamusoro nekuda kwekuvandudzwa kupatsanurwa.
Carrier Gasi Kuyerera Rate: Iyo inoyerera chiyero cheGasi inotakura inobata kuti kukurumidza kuongorora sei kuongorora kuburikidza nekhoramu. Chiero chakakwana chinovimbisa mapundu anopinza; Yakanyanya kukwirisa chiyero chinoyerera chinoguma nekuyamiswa kwekudyidzana pakati peiyo ana kana stationary chikamu, zvichikonzera munzvimbo diki dzepamusoro.
Iwe unoziva mutsauko pakati peHPLC vials uye gc vials? Tarisa chinyorwa ichi:Ndeupi musiyano uripo pakati peHPLC vials uye gc vials?
3. Detector marongero
Iko kuita kweiyo detector kunokanganisa zvakananga iko kurongeka kweiyo yepamusoro nzvimbo yekuyera kwenzvimbo:
Detector Sensitivity: dzakasiyana dzekuona dzakasiyana siyana kuti dziongorore. Semuenzaniso, murazvo wonization ionization (FID) inotarisisa, asi inogona kusapindurudza zvakaenzana kune ese makomisheni, inokanganisa nzvimbo yakayerwa.
Nguva yekupindura: Nguva yekupindura yekuteerera inokanganisa kuti inokurumidza sei kuchinja mukuongorora kwekuongorora. Nguva dzinononoka dzekupindura dzinoguma dziitwa mumapepa emahara, ayo anoderedza nzvimbo yakaverengerwa.
Tembiricha Yakasimba: Vazhinji vanoongorora vanoteerera kuchinjika kupisa. Kuona tembiricha yekushandisa inoshanda inobatsira kuchengetedza mhinduro isingaenderane uye kuyerwa kwakakwana kwenzvimbo yePeak.
4. SAMPLE HUTI
Chimiro chemuenzaniso chiri kuongororwa zvakare chinoshanda chaizvo:
Kusimbisa: Kusvinwa kwekuziva zvakananga kunokanganisa nzvimbo yepamusoro; yakakwira kusunganidzwa, iyo yakakura nzvimbo yenzvimbo. Nekudaro, kana fungidziro ikapfuura mutsara wedzinza renzvimbo, uye zviyero uye zviyero zvisina kunaka zvinogona kuitika.
Matrix Mhedzisiro: Kuvapo kwemamwe makomputa mune yakaoma matrix inogona kukanganisa nekuona ongororo. Matrix zvikamu zvinogona kutora kana kuita neye analyte, zvichikonzera kuderedzwa kwenzvimbo yePeak kana kuti yakachinjirwa nguva dzese.
Kuwedzera: Iyo nyonganiso yekuongorora inokanganisa maitiro ayo panguva ye chromatography. Zvakawanda zvisingaenzaniswi mishonga yeElute nekukurumidza uye inogona kuburitsa zvakapatsanurwa mapepa kupfuura zvisinganzwisisike zvisingaiti makomputa, izvo zvinogona kuguma nekuwedzera misoro nenzvimbo dzakaderedzwa nekuti nguva dzekuchengetwa dzakatambanudzwa.
5. Maitiro ekuita
Kuwedzeredza system performance kwakakosha kune chaiyo gc mhinduro:
Baseline ruzha: Yakakwira Baseline ruzha runogona kuvhara zvidiki zvidiki kana kuderedza saizi yavo inooneka, ichikanganisa kurongeka kwekutaurirana uye kutungamira kune zvikanganiso munzvimbo dzepamusoro.
Column Kubudirira: Yakaderedzwa Column Kubudirira nekuda kwekukwegura kana kusvibiswa kunogona kuguma nemapepa akawandisa uye nzvimbo diki. Kugara uchichengetera uye kuongorora kwekuita kwecolum kwakakosha kuwana mhinduro dzisingaperi.
Kubatanidzwa paramita: Iyo software inoshandiswa kuunganidza mapessiji anofanirwa kugadzirirwa nenzira kwayo. Zvigadziriso zvisina kunaka zvinogona kutungamira kune isina kunaka peak nharaunda kuverenga nekuda kwekuisa kusiri kusiririsa kusiririsa kana kubvunda zvikumbaridzo.
Unoda kuziva zvakawanda nezve mutsauko pakati peLC-ms uye GC-MS, ndapota tarisa chinyorwa ichi: Ndeupi musiyano uripo pakati peLC-ms uye GC-MS?
Mhedziso
Nzvimbo dzeGC Peak dzinokanganiswa nezvinhu zvakasiyana siyana, kusanganisira jekiseni maitiro uye column mamiriro, detector marongero, uye maitiro ekuenzanisira. Kuve nechokwadi chekukwirisa-mhando data, vanotsvaga zvine mutsindo kudzora jekiseni jekiseni uye kumhanya, vhura column mamiriro akadai sekupisa uye kuyerera kwemari, uye chengetedza chiitiko chekuongororwa kuburikidza nenguva yekusagadzikana. Ivo zvakare vanofanirwa kufunga nezve sample matrix mhedzisiro kana uchigadzirira samples. Nekugadzirisa idzi nyaya, mabara anogona kuvandudza kurongeka uye kuvimbika kwe chromatographic ongororo, pakupedzisira kuwana zvimwe zvakavimbika zviwanikwa.
Chirungu
ChiChinese
