Sep. 8th, 2023
Chromatography ndeyechinhu chakasarudzika chekuongorora chinoshandiswa nemasayendisiti kupatsanurana uye kuongorora musanganiswa wakaoma emakomputa. Mhedzisiro chaiyo yakakosha mumishonga yemishonga, dzidziso dzekutsvagisa, marongero emhando yepamusoro kana masangano emhando yemhando yakafanana; Mumwe anowanzo kufuratira chikamu che chromatography ari kuzadza vials nenzira kwayo yekuedza kushandisa iyi nzira. Isu tichakurukura kukoshesa kwaro pano pamwe nemirairo inogona kubatsira kuve nekuita zvakaringana mhedzisiro kana ichiita kuti yako chromating yako.
Nei kuzadza kwakakodzera kwechiitiko kwakashora
Zvakarurama uye kubereka chromatographic mhedzisiro, kusanganisira mhando yemhando, kusarudzwa kwekoramu, kushandiswa kwechiridzwa, kusarudzika kwekugadzirira, pakati pezvakawanda, pakati pevamwe. Chimwe Chinhu Chakakosha Chinogona Kuchinja Mhedzisiro Yako Zvakanyanya Kuita Maitiro aunoita iwe uye gadzirira chromatography vials - pano ndosaka izvi zvinhu:
SAMPLA TERTE:Vakunda vasina kufanira vanokwanisa kutungamira kumanikidza kusvibiswa kana kubuda kwemvura, vachishandura kuumbwa kwayo uye kuchinja zvabuda.
Kubereka:Kuenderana kuri pane iyo core ye chromatography. Kuita zvinhu zvakakodzera vial kuzadza maitiro anoona vial imwe neimwe inobata huwandu hwakaenzana hwemuenzaniso kuti uwane zvimwe zvinodzokororwa.
Baseline Kugadzikana:Kuita kuti kuzadza kwakakodzera kunogona kubatsira kuchengetedza bhasikoro rakasimba, kuderedza ruzha uye kupindira mune yako Chromatorm.
Dziviriro yeColumn:Kuzadza kwechokwadi kusingaperi kunogona kudzivirira mhepo mabhuru emamhepo kana splans splatter kubva mukuunzwa mune yako chromatography column uye kukuvadza munzira chero ipi zvayo.
Nhanho-ne-nhanho gwara rekuzadza zvakanaka chromatography vials
Zvino zvatinotenda nei kuzadzachromatography vialsZviripachena ndezvekukosha kwakadaro, ngatiende kuburikidza nedanho rega rega rinodiwa kuti tizviite nemazvo:
Nhanho 1: Sarudza kuti ndeipi yaunoda iwe yaunoda
Sarudza vials zvinoenderana neyako chromatography system uye samples, kusanganisira chero ma samples-inonyanyoka samples kana zvikumbiro zvinowanikwa vadiki vakajeka vanogona kubatsira. Zvakare ona kuti vakachena uye vakasununguka kusvibiswa.
Chromatography mavapu akauya mumhando dzakasiyana-siyana, zvigadzirwa, uye kuvharwa. Size yakajairika ndeye 1.5 ml uye 2 ml, kunyangwe yakakura kana madiki mavhoriyamu inogona kushandiswa zvichienderana nezvinodiwa zvako. Viums anowanzogadzirwa kubva kugiroshilthe girazi, iyo inopa makemikari kurwisa uye yekudzikisira kudzikama, kana kubva padzimba remanyorerwo kune zvimwe zvekushandisa zvinoda mapurasitiki.
Paunenge uchisarudza vial, funga:
Kuenderana neyako sampuli:Ita shuwa kuti zvinhu zvekusuwa hazviite kana kusvibisa muenzaniso wako.
Vhoriyamu Inodiwa:Sarudza vial iyo inogona kubata yako sampuro isina njodzi yekupaza kana kubuda kwemvura.
Yevhavha Type:Kunyangwe zvimedu zvechema, kana snap caps, kana crimp caps, ita shuwa kuti kuvharwa kwakachengeteka uye kwakakodzera nzira yako yekuongorora.
Nhanho yechipiri: Label yako vials
Labelding vialsZvakanyatsonzwisisika kunokosha pakutengeserana uye kudzivirira kusanganisa-ups panguva yekuongorora. Shandisa mari yemvura yemvura kana mapepa ekuratidzira kuti ave nechokwadi chekuti ruzivo rwekuriridzira runoramba uchiita zvine musoro pakubata uku, senge zita raro, zuva rekuongorora pamwe nechero basa rakakodzera kana rakawanda nhamba.
Nhanho 3: Ronga kugadzirira kwemuenzaniso
Kugadzirira samples nenzira kwayo kwakakosha kuwana chaiyo chromato apera. Ive neshuwa yekuvagadzirira nenzira yekuti vakakodzera jekiseni mune chromatography system - izvi zvingabatanidzwazve, nguva dzose kutevedzera maitiro ekushanda
Unoda kuziva nzira yekusarudza crimp vial vs. snap vs. screw cap vial ?, Tarisa chinyorwa ichi: Crimp vial vs. snap vial vs. screw cap vial, maitiro ekusarudza?
Nhanho 4: Sarudza iyo yakakodzera pipeette
Kuwana uye kuchengeta zvakanaka pipet yakakodzera kunonyanya kukosha. Sarudza pipette ine vhoriyamu renji iyo inoenderana neyako yemuenzaniso inotarisirwa vhoriyamu; calibrate uye simbisa kurongeka kwayo pane yenguva dzose yekuve nechokwadi chechokwadi. Shandisa matipi akakodzera pipette ekuderedza kurasikirwa kana kuyambuka-kusvibiswa pakati samples.
Nhanho 5: Kubata Vals Nekungwarira
Kuchengeta nharaunda yakachena kunokosha kuti udzivise kusvibiswa. Usati watanga basa, ronga uye chenesa yako yekushanda usati waburitsa mapurabheti magurovhosi kuti uderedze njodzi yekusvibiswa. Kana netsaona kubata mukati memukati kana rimani yeiyo vial netsaona, rasa uye shandisa imwe vial pachinzvimbo kudzivirira sampula.
Nhanho 6: Zadza VIAL NEMAHARA
Kubata vial padanho remaziso kunobatsira kuti uve nechokwadi chekuti iwe unosvika pakuzadza kwakakodzera mutsara uye kuti hapana mhepo mabhurugwa yakabikwa mukati memoyo wayo.
Kana pipetting samples, sunungura plunger zvishoma nezvishoma kudzora kuyerera kwemvura uye kudzivirira kupururudza izvo zvinogona kuguma nehari isingaenderane.
Regedza kushandisa simba rakawandisa kana pombetting sezvinoita izvi zvingaunza zvisingadzivikanwe mumhedzisiro yako. Panzvimbo iyoyo, shandisa nyoro uye yakatsiga pombiet maitiro.
Nhanho 7: Kiya vial yako zvakachengeteka
Caps kana septa yakagadzirirwa zvakananga kuti Chromatography yako application inofanira kusarudzwa. Vakasungwa vane chishuwo vanosungwa uye vanochengeta kutendeka kwematsi; Kupfuura-kusimbiswaCAPSaigona kukuvadza vials kana kukanganisa chisimbiso chaiko; Kuti uwane mhinduro dzakanakisa dzinotevedzera mapundu emarabherator yemarabheratari pamakona emuchadenga.
Nhanho 8: Sanganisa kana zvichidikanwa
Kuve nechokwadi kana kugoverwa pasina mabhuru emhepo, izvo zvinokosha kuti zvigadzikiswe zvakasimba kana zvikamu zvinogona kugara panguva yekuchengetedza zvakanyatsosanganiswa muzvikamu zvakati wandei Inversion ichave nechokwadi chekuparadzira isina kumisikidzwa kwemhepo kuiswa mumusanganiswa.
Nhanho 9: Unganidza iyo sampula ruzivo
Real realkodhi-kuchengetedza ndiyo kiyi yedata yekutengeswa uye kuongororwa. Chengetedza bhuku reRababhoritari reABuratory rinosanganisira ruzivo nezveMuenzaniso wega wega wakatorwa
Nhanho 10: Kuchengetedza kwakakodzera
Kuti uchengetedze kutendeka kwemuenzaniso wako, chiise mumamiriro ezvinhu akakodzera kuchengetedza kwayo. Mamwe ma samples anoda kupirisitiji vamwe vachifanira kuchengetwa pamhepo tembiricha kana kuchengetedzwa kubva pachiedza. Bvunza yako marabhoritari nhungamiro uye maitiro akanakisa maererano nekuchengetedza sampull emhedzisiro yakanakisa.
Zvakakodzera kuzadza chromatography vials kwakakosha kuti uzadzise zvakaringana uye mubereko zvinoitika mukuedza kwako kuongororwa. Nekubhadhara hanya nekunyatsotarisisa uye nekutevera zvirevo zvakatemwa, iwe unogona kuderedza zvikanganiso, ita kuti kutendeka kwemuenzaniso uye utsigire zvakakwirira zvemhando mune chromatographic kuongorora. Rangarira kuti kunyatsoita mumuenzaniso unobata zvakananga zvinokanganisa mhedzisiro inowanikwa mune yako ongororo.
Tora zvakazara nezvinonzwisisika mumakumi mashanu emhando dzeHPLC LPLC vial mibvunzo nenyaya yedu inodzidzisa. Vhura mhinduro dzaunoda:50 Mibvunzo inowanzo bvunzwa paHPLC vials
Chirungu
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