Nei GC-MS Inoshandiswa pakuongororwa kwezvinodhaka?

Gasi Chromatography-Mass SpectRogry (GC-MS) inozivikanwa zvakanyanya maitiro ane imbonzi ane kuongororwa zvinodhaka, kunyanya muminda yekiriniki uye yekufemerwa. Kugona kwayo kupa zvakaringana, zvinonzwa, uye zvakavimbika mhedzisiro zvinoita kuti ive nzira yesarudzo yekuona uye kufungidzira zvinodhaka uye metabolites yavo mumasangano ezvipenga. Iyi blog ichatsvaga zvikonzero zvekushandisa GC-ms zvekuedza zvinodhaka, kutarisa nezve zvakanakira izvo, nzira, uye zvinoshandiswa.

Unoda kuziva zvakawanda nezve mutsauko pakati peLC-ms uye GC-MS, ndapota tarisa chinyorwa ichi: Ndeupi musiyano uripo pakati peLC-ms uye GC-MS?

GC-MS Nzira

GC-MS inosanganisa nzira mbiri dzekuongorora: gasi chromatography (GC) uye misa spectrometry (ms).

Gasi Chromatography: Mune iyi nhanho yekutanga, sampuli inovhuvhurwa uye yakaparadzaniswa mune izvo zvega zvinhu uchishandisa calumn column. Kuparadzanisa kunoenderana nepfungwa yekubika uye porto remagoma, kubvumira kupatsanurwa kwakashanda kwemusanganiswa wakaoma.

Massent SpectRocressry: Kamwe zvikamu zvakaparadzaniswa, ivo vanounzwa mune misa misa spectrometer. Pano, ivo vane ionized uye izvo zvinokonzerwa ion dzinoongororwa zvichibva pane yavo yakawanda-kune-yekubhadhara ratio. Iyi process inogadzira yakasarudzika mashandiro emhando imwe neimwe yakanangana, ichipa zvese zvinokatyamadza uye huwandu hwehuwandu.

Iyi nzira-nhanho nzira inobvumira kuzivikanwa chaiko kwezvinhu zviri mumuenzaniso, kugadzira GC-MS kunyanya yakanyatsokodzera kuongororwa kwezvinodhaka.

Zvakanakira GC-MS mune zvinodhaka bvunzo

1. Kukwikwidzana kwakanyanya uye chokwadi

Chimwe chezvikonzero zvikuru zvekushandisa GC-ms mukuedzwa kwezvinodhaka ndiko kwekunzwa kwayo kukuru:

Yakaderera yekuona kuongororwa: GC-ms inogona kuona zvakanyanyisa kuderera kwemishonga, kazhinji muNanograp \ / ml range. Kugona uku kwakakosha mumakirinari marongero, uko varwere vanogona kunge vatora zvidiki zvezvinodhaka kana metabolite.

Chaicho chitupa: misa masendimita anopa ruzivo rwakadzama pamusoro peiyo molecular chimiro chemubatanidzwa, zvichiita kuti kuitisa kuzivikanwa chaiko kunyange pakati pezvinyorwa zvakafanana. Uku kufungidzira kunobatsira kuderedza mapepa enhema izvo zvinogona kuitika neimwe nzira dzekuongorora.

2. Yakarongeka yekuongorora kugona

GC-MS inokwanisa kuongorora zvinhu zvakawanda:

Multi-Dish Dishing: Iyo tekinoroji inogona kujairika kuongorora zvinodhaka zvakawanda uye yavo metabolites mune imwechete muenzaniso. Uku kugona kwakazara kwakakosha mukukakavara mune zvekiriniki zvine mwero, uko varwere vangave varipo kune akasiyana siyana.

Shanduko yezvinhu zvitsva: Sezvo madhiragi matsva anouya kumusika, zvinhu izvi zvinogona kuiswa muyedzo protocol yeGC-ms nekuvandudza nzira iyo nzira kana raibhurari database inoshandiswa kuzivikanwa.


Iwe unoziva mutsauko pakati peHPLC vials uye gc vials? Tarisa chinyorwa ichi:Ndeupi musiyano uripo pakati peHPLC vials uye gc vials?

3. Kuongororwa Kwekuedzwa

Nepo mafungu ekutanga ekuongorora, akadai se asina kujairika, anogona kuratidza kuvapo kwemushonga, kazhinji kazhinji hazvigone kuratidza kuti:

Kuongororwa kusimbisa: GC-MS inogona kushandiswa seyekubvumirwa bvunzo mushure mekutanga kuongorora. Migumisiro yakanaka kubva kune immunassay inogona kusimbiswa neGC -Ms kuongorora, kupa iyo inodiwa yekugadzirisa chiratidzo chezvisarudzo zvepamutemo kana zvisarudzo zvepamutemo kana zvekiriniki.

Kutevedzera Kwemutemo: Mumatare ekurongedza, masangano ekudzora anowanzoda kusimbisa nekuedzwa nenzira dzakadai seGC-MS kuve nechokwadi chekurongeka uye kuvimbika kwemhedzisiro yezvinodhaka.

Zvikumbiro zveGC-ms mune zvinodhaka bvunzo

1. Clinical texicology

Mune clinical texicology laboratories, GC-ms inowanzoshandiswa kuongorora urini samples yezvinodhaka zvekushandisa zvisina kufanira:

Zvidhori zvezvinodhaka uye zvehupfumi zviitiko: Inotora basa rinokosha mukuongorora varwere neakashandurwa chimiro chepfungwa nekuda kwekufungidzira zvinodhaka kana chepfu. Nekuzivisa zvinhu zvakananga zviripo muUrine, vanorembera vanogona kuita sarudzo dzekurapa dzine ruzivo.

Kuongorora mishonga yezvinodhaka

2. Kutsvaga mafomu

GC-ms inowanzo kushandiswa zvakanyanya kudzidza zvinodhaka metabolism uye pharmacokinetics:

Kuongorora metabolite: Vatsvagiri vanoshandisa GC-MS kuongorora metabolites yakagadzirwa mushure mekutonga zvinodhaka, izvo zvinobatsira kunzwisisa kuti zvinodhaka zvinogadziriswa mumuviri.

Kuvandudza kweNew Ongororo Nzira: Iyo Kuchinjika kweGC-MS inobvumira vaongorori kuti vatore nzira nyowani kana matrices, nokudaro achivandudza kugona kwekuongororwa kwezvinodhaka.

Mhedziso

Gasi Chromatography-Misa SpectRogry (GC-MS) yave yegoridhe tekinoroji yekuedzwa zvinodhaka nekuda kwekunzwa kwayo kukuru, kufungidzira, uye zvakazara kugona kwekutarisa. Kugona kwayo kupa mhinduro dzakakwana kunoita kuti igoneke mune ese ekiriniki toxicology uye kuongorora kwekufungidzira. Sezvo mishonga mitsva enderere mberi nekukudziridza uye kukudziridza, kushandurwa kweGC-MS kunovimbisa kuti kucharamba kuchiramba kuchiramba chiri pamberi pekuongorora kwezvinodhaka.

Nekushandisa zvinobudirira zveGC-ms tekinoroji, marekodhi anogona kuwedzera kugona kwavo ingafungo apo anoona mhedzisiro dzakaringana uye dzakavimbika dzinokosha mukuchengetedza kushivirira uye kutevedzera zviri pamutemo.


Kuti uwane rumwe ruzivo nezve autosamupler vials yegesi chromatography, kureva chinyorwa ichi: 2 ml autosamppler vials yegesi chromatography