Mukuongorora kwekuongorora, HPLC inzira yakakosha. Iyo inoparadzanisa, inozivisa, uye kuyera zvikamu zvemusanganiswa. HPLC fungwa yekuongorora haina kutsamira pakutsanangurwa kwesistimu. Izvo zvakare zvinoenderana nemhando uye zvakakodzera kune zvinokanganisa zvinoshandiswa. Izvi zvinonyanya kuitika kune HPLC vials.

Basa Rinokosha reHPLC Vals

HPLC vialsndiwo mudziyo mukuru wekuchengetedza sampula. Ivo zvakare vanoisa samples mu chromatography system. Sarudzo yeMial haisi shoma. Icho chisarudzo chinogona kukanganisa mhedzisiro uye kuvimbika. Varume vanofanira kuchengeta samples vakachena. Vanofanirawo kumisa kusvibiswa. Uye, ivo vanofanira kupukunyuka kushushikana kweHPLC.

Unoda kudzidza zvese nezveHPLC vials? Tarisa uone Ichi Chinyorwa chakazara:Encyclopedia of Hplc vials.

Kukosha Kwekuchengetedzwa Kwekutanga

Kutendeka kwemuenzaniso kuri kukanganisa muHPLC ongororo. Iyo nzira samples yakachengetwa isati yaongororwa inogona kukanganisa mhedzisiro. Kuchengetedza murombo kunogona kukonzera kukuvadzwa kwemuenzaniso. Inogona kukonzera kusvibiswa kana kushandurwa. Idzi nyaya dzinogona kukuvadza kuongorora kwechokwadi. Saka, zvakakosha kuti usarudze HPLC vials. Ivo vanofanirwa kupa yakagadzikana uye inert yekuchengetedza.

Sampula kugadzirisa chokwadi

Kugadzirira samples ndeyechokwadi. Inofanira kuitwa pamberi pekutanga kwavo kuHPLC system. Kuita uku kunosanganisira kudhura, kusanganisa, uye filtration. Vanofanira kuitwa nekunyatsofungisisa kuti vadzivise kuwedzera kusiyanisa kune ongororo. Yakanaka HPLC vials ndiyo kiyi. Ivo vanomira kufambidzana pakati pemienzaniso uye vial zvinyorwa. Kubatanidzwa kwakadaro kunogona kuchinja muenzaniso.

SAMPLE INJECE CHETE

Iko kurongeka kwejipple jekiseni ndiyo kiyi inosarudza kuvimbika kweHPLC mhinduro. Iyo sampuli yemuenzaniso mune chromatography system inofanira kunge iri chaiyo. Izvo zvinofanirwawo kunge zvakafanana nguva imwe neimwe. High-mhando Hplc vials dzakagadzirirwa kuchengetedza iyo sampuli inzwi rakafanana. Dhizaini yeVial inofanira kuenderana neshoko rezvinoyevha.

Uchinetseka kuti ungageza zvakanaka sei yako HPLC vials? Tarisa uone chinyorwa ichi chakadzora:Nzira shanu dzekuchenesa dzeHplc vials.

Hplc Val Sarudzo

Kusarudza uye kushandisa HPLC vials ndeyekutsoropodza. Ivo vanove nechokwadi chekuti chokwadi uye kuvimbika kwe chromatographic mibairo. Kusarudza iyo chaiyo yekudzikisa njodzi yengozi yemuenzaniso. Izvo zvakare zvinodzivirira kurasikirwa kwemuviri. Izvi zvinochengeta mhedzisiro inoenderana uye inobereka.

Makemikari zvivakwa zvemuenzaniso

Chemakesi yemakemikari yemuenzaniso chinomiririra sarudzo yeiyo vial zvinyorwa. Acicic kana alkaline samples inogona kunyorova girazi. Izvi zvinoreva kuti iwe unofanirwa kushandisa mapurasitiki emapurasitiki kana girazi makamuri akavezwa kuti adzorere kurongeka. Yemasampuli ine organic sols, iyo vial zvinyorwa inofanira kudzivisa izvi soles. Izvi zvinodzivirira kubuda kana kuita.

Ongororo dzekunakidzwa

Sarudzo yeMial inogona kukanganisa manzwiro ekunzwa. Izvi ndezvekuongorora zvidiki zvidiki, sevhu biommarkers kana zvinodhaka zvasara. Sielaned vials inogona kuderedza sampule adsorption pamadziro emukati memukati. Izvi zvinowedzera kusaona kwekunzwa.Pre-cuka septumvials vakatema kurasikirwa kwekuenzanisira panguva yekuzvidza, vachiratidza kuongororwa kwakarurama.

Isina chokwadi kuti usarudze pre-slit seka kana kwete kune yako HPLC vials? Tarisa uone chinyorwa ichi:Ungasarudza sei Septa: Pre-slit kana kwete?

HPLC system kuwirirana

Iyo saizi uye yekuvhura mhando yeiyo vial inofanirwa kuenderana nejekiseni yeHPLC system. Miromo mikuru yemuromo yakakwanwa yakakodzera masampula. Zvakare kubatsira masampula zvine zvimedu zvakasikwa. Inoderedza njodzi yekuvhiringidzika panguva yekukura. Standard Bhodhi Mabhodhoro akakodzera kune mazhinji emazuva ese anoshandisa.

Kufambira mberi kwehunyanzvi mukugadzira vial kugadzira

Masayendisiti akagadzira kukosha kwehunyanzvi hwekugadzira hplc vials. Vagadziri vava kupa mabhodhoro ane zvivakwa zvakavandudzwa. Izvi zvinosanganisira adsorption yakaderera,kupora kwakanyanya, uyePre-Slit Septa. Izvi zvigadzirwa zvinogadzirisa zvinodiwa nekuchinja kwekuongorora kwekuongorora. Inogonesa kuongorora kwakakwana uye kwakarurama.

Standard HPLC vial zviyero uye maficha

Vagadziri vematunhu eHPLC vial zviyero kuti vave nechokwadi chekuita nemhando dzakasiyana siyana.

1. 8x40mm (1ml) vials, kazhinji ne screw kapu, yakakodzera kune madiki ekuenzanisira mavhoriyamu.

2. Vials iri12x32mm (2ML) vials, saizi yakajairwa. Ivo vanowanikwa neCSGREA CAPS kana kumanikidza mapepa. Iwo macaps ndeye mwero wakavhurika kana wakakurisa.

3. 15x45mm (4ML) vials, yakagadzirirwa kune yakakura sampuzi, inowanzove yakavhurika pachena.

Hplc Val zvinhu

Hplc vials yakagadzirwa negirazi yakajairika, epurasitiki, uye zvinhu zvakakosha. Muenzaniso ndeye polymer-yakafukidzwa girazi, iyo inowedzera kugadzikana uye kusimba. Kune zvimwe zvinoshandiswa, kunge protein kuongorora,mapurasitiki vevoniCheka sampule adsorption uye kusvibiswa. Mazhinji standard HPPLC uye LC-ms anoongorora anofarira girazi vials. Izvi zvinodaro nekuti zvakagadzikana. Varume mapurasitiki vakanaka kune samples isingade kukwirisa kwakanyanya. Ivo vaivhenekera uye vasingakwanisi kutyora. Sielaned vials inokatyamadzwa pamusoro pekurapa. Inowedzera kunzwa kwekushandiswa kwakananga.

Kusimbisa iyo vial

Kuiswa chisimbiso chaiko kweHPLC vials kwakakosha kuti udzivise muenzaniso wekubereka uye kusvibiswa. Screw cap vials ndeyekubatsira kwekuedza nekuchinja kwakawanda kwekutanga. Vials neyekudzvinyirira-pamapundu vane chisimbiso chakachengeteka. Iwo akanaka pakuchengetedza kwenguva refu kana kutakura. Pre-akacheka septum vials anogadzirwa kubatsira anozivikanwa anotarisana. Ivo vanocheka njodzi yemuenzaniso wekuenzanisira-kusvibiswa uye kurasikirwa.

Uchinetseka kuti ndezvipi zvinhu zvekufunga kana uchisarudza autosampler vial? Tarisa uone chinyorwa ichi chakadzora:Mapoinzi mashanu ekufunga kana uchisarudza autosampler vial.

Mhedziso

Kusarudza uye kushandisa HPLC vials yakakosha. Ivo vanokoshesa kubudirira kwe chromatography. Kusarudza izvo zvakarongeka zvakakosha. Vanofanira kuenderana nemakemikari emuenzaniso, kunzwa kwekunzwa, uye HPLC system. Kuita izvi kunobatsira vatsvagiri kunatsiridza chokwadi uye kuvimbika kwemhedzisiro yavo. Kuteerera zvakadzama mune vial sarudzo ndiyo kiyi. Inoratidza basa rakarukwa reHplc vials mukuwana kunaka.

Yapfuura:

Asingaenzaniswi kuishaisa chisangano: Sei septa kusagadzikana kukanganisa muenzaniso kuratidzwa kwemuenzaniso uye nekuedza kurongeka

Sarudza iyo yakakodzera Tsika HPLC vials kuti uwedzere yako HPLC ongorora