Chii chinonzi HPLC yakanaka?

I. Sumo

HPLC, kana yakakwirira-perform yemvura chromatography inoshandiswa zvakanyanya mumakemikari ekuongorora kuti azviparadzanise uye kuratidza zvikamu zvebvunzo yebvunzo. HPLC mhedzisiro inoenderana nemidziyo inoshandiswa, kusanganisira vials inobata samples. Yechokwadi uye yakavimbika HPLC mhinduro, zvakakosha kuti usarudze zvakanakaHplc Vial.

Chinyorwa ichi chinosimbisa pfungwa dzakakosha zvakanyanya pakusarudza Hplc vials. Tichatarisa kune akasiyana mhando dzeHPLC vials uye zvavanonyanya kufanana. Isu tichazokupawo mazano ekusarudza uye kushandisa HPLC vials yekuita basa rakakwana uye nekubuda kwakakwana.

Ii. Mhando dzeHPLC vials

Varume vegirazi ndidzo dzinonyanya kushandiswa mhando yeHPLC vials, pavanenge vachipokana nemakemikari akanyanya, akaderera Iyo yakajairika zvinhu zvinoshandiswa kune HPLC vials ndeye borosiliprate, nekuda kwekupokana kwayo kune inopisa uye yemakemikari kurwisa. Amber girazi vigial dzakanakira samples dzinotarisisa uye ugopa kudzivirirwa kukuru kubva pachiedza. Nekudaro, ivo havana kujeka kupfuura girazi rakajeka vigial.

Mapurasitiki emapurasitiki zvakare ane yakanyanya kudiwa nekuda kwehuremu hwavo hwekurema uye nekukasira. Polypropylene vials inowanzo shandiswa. Vanogona kuwirirana nemazvo manyorerwo kana organic sol sol sol sol sol. Ivo vakagadzirwa nePolyethy (pe), iyo yakakodzera kuchengetedza uye kutakura samples, asi kwete yeHPLC nekuda kwekudzikisira kwemakemikari akaderera.

Zvinyorwa zvinoshandiswaHplc Vialinotsanangurwa nemhando yemuenzaniso, iyo nzira yekuona, uye iyo chromatography nzira. Kuti udzivise kusvibiswa, zvakakosha kuti uve nechokwadi chekuti iyo vial zvinhu inoenderana nemidziyo kana reagents iri kushandiswa.

III. Chii chinonzi HPLC yeHPLC vial ??

Iyo yakanaka HPLC vial inofanirwa kuenderana nemagetsi anoshandiswa mune chromatography maitiro, ana yakaderera inobvisa \ / lecraable mazinga, uye huwandu hwekugadzira hunogadzira. Kuti udzivise kurasa kwemuenzaniso panguva yekuongorora, vials inofanira kukodzera nemazvo nemidziyo ye chromatography uye accessories semapaketi uye Septa.

1.comPattibility ye solvent

HPLC vials inofanira kuenderana nemagetsi ari kushandiswa kwe chromatography. Solvents inogona kushatisa vial zvinhu kana kukonzera zvinoshatisa kuti leach kunze, izvo zvinogona kukanganisa mhedzisiro uye sampuli. Girazi vials dzinowanzo kuve dzinoenderana nemarara. Mimwe vadiki mapurasitiki ayo anoenderana nemarara emhando yakakura iripo.

2.w Inobvisa \ / llechable

Mapepa anokwanisa kuburitsa makomputa kubva kune iyo vial mumuenzaniso uchibvisa zvinobuda zvinogona kuburitsa makomendi mune solvent. Ose ari maviri anogona kutungamira kune enhema dzakanaka kana dzenhema dzakashata mhedzisiro, ichiita kuti ikakosha kusarudza vials ine yakaderera inobvisa \ / lechable. Magirazi magirazi anozivikanwa kuti ane mwero akaderera ezvekutora \ / lecauble kupfuura mapurasitiki.

3.Consclest kugadzirwa kwehunhu

Iyo HPLC vials 'yekugadzira maitiro inogona kuve nekukanganisa kuita kwavo pamwe nekuvimbika kwemhedzisiro yekuongorora. Iwe unofanirwa chete kutenga vials yakagadzirwa nevanogadzira vane mbiri yakanaka yekudzora kwemhando. Izvo zvakakosha kusarudza vials kubva kuVagadziri vane mukurumbira wekutonga kwemhando uye kuomerera kune maindasitiri zviyero.

4.Rhokichography system inofanira kuve yakakodzera zvakanaka kune kamera.

Hplc Vialinowanikwa mune dzakasiyana-siyana magadzirirwo anosanganisira screw kumusoro, snap yepamusoro uye shell vials. Imwe neimwe ine yayo chaiyo yakasarudzika maitiro. Kana uchisarudza vials, zvakakosha kuti uve nechokwadi chekuti vanokodzera neichography system pamwe nezvinhu zvemagetsi uye Septa. Viumus vacho vanofanira kukwana nenzira kwayo kuve nechokwadi chekuti ivo vanozvisimbisa nemazvo uye kuti hapana sampuli yakarasika panguva yekuongorora.

DEELVE kupinda munyika ye 2ML 9mm HPLC vials neiyi chinyorwa chinodzidzisa. Ongorora zvavaiita uye zvinoshandiswa mukuongorora kweMakemikari:1.5ML 9mm pfupi pfupi tambo yangu vial nd9

Iv. Iyo yakakodzera kubata uye kuchengetedza yeHPLC vials

Kuve nechokwadi chechokwadi uye chakavimbika mhedzisiro, zvakakosha kubata uye kuchengetedza HPLC vials nenzira kwayo. Heano mashoma mashoma maitiro aunofanira kufunga nezve:

1. Dzivisa kujekeswa kwechiedza: Chiedza chinogona kukanganisa mamwe samples. Chitoro mavanga munzvimbo dzine rima kana kufukidza uchishandisa zvinhu zverima.

2. Vials inofanira kuchengetwa mune chaiyo tembiricha yekupisa. Kupisa kwakanyanya kunogona kukanganisa maitiro emasampuli.

3. Ngwarira kunyorova. Kunyorova chinhu chinotungamira chekusvibiswa. Izvo zvakakosha kuti zvitoro zvirimo munzvimbo dzakaoma.

4 Iwo macaps anogona kutyora kana akawandisa akasungwa. CAPS inofanira kungosimbiswa kusvika padanho rekugadzira chisimbiso.

5. Mazano akanyatsojeka nezita remuenzaniso, zuva, uye chero ruzivo rwakakodzera.

6. Usati washandisa vials, zvakakosha kuti uzviongorore nekuda kwekukuvara kana kuremara.

Izvo zvakakosha kuti ushandise yakasimbiswa vials kuti ive nechokwadi chekuti vanoenderana neiyo chromatography system uye kusangana zvakatemwa. Kubata kwakakodzera uye kuchengetwa kweHplc vials kunogona kubatsira kudzivirira kusvibiswa uye kubereka mibairo chaiyo uye yakavimbika.

V. Zvakanakisa maitiro eHPLC Val shandisa

Izvo zvakakosha kuti ushandise vlc vials kuitira kuti vaite zvakakwana uye vape mibairo chaiyo. HPLC vials inofanira kuchengetwa kubva pakupisa kupisa uye zuva. Kunyorova uye kuratidzwa kwechiedza kunogona kukanganisa chokwadi uye mhedzisiro yemhando.

Shandisa chete zvakaratidzwa vials. Varume vakasarudzika vakaedzwa kuita uye mhando. Aya makomels anogona kushandiswa nzira dzekuongorora dzinoda kurongeka uye kunyatsojeka.

Zvinyorwa zvakaringana: Zvakakosha kuti zvinyoronyoro zvakaringana neruzivo rwakakodzera, sekuti zita remienzaniso, kusangana uye zuva rekugadzirira. Izvi zvichabatsira kudzivisa kuvhiringidzika kana kukanganisa kana uchiongorora.

Tarisa vials usati washandisa chero kukuvara kana kuremara. Chero maviru ane zviratidzo zvekukuvara kana kuremara kunofanira kuraswa.

Kutevera aya maitiro akanakisa anobatsira vashandisi kuve nechokwadi ichoHplc Vialinogona kushandiswa nenzira inowedzera kurongeka uye kuita mu chromatography kuongorora.

Vi. Maitiro ekusarudza iyo yakakodzera HPLC vials

Izvo zvakakosha kuti ufunge nezve mhando yemuenzaniso uye kuenderana ne solvent kana uchisarudza Hplc vials. Kana iyo sampu iri acidic, iri nani kushandisa vials of borosililiate, iyo inodzivirira kurwisa kwemakemikari kupfuura mamwe marudzi egirazi. Kana iyo sampuli isiriyo saka vials yakagadzirwa kubva kuPolypropylene kana zvimwe zvinhu zvine yakaderera adsorption capitiities inogona kuve iri nani.

Bhajeti chimwe chinhu chinofanira kufungwa nezvazvo. Kunyangwe mamwe marudzi ari kudhura kupfuura mamwe, mutengo wakakwana we chromatographhy hauna kukanganiswa nemutengo weiyo vial. Vasingaenderani kana vakaderera-zvemhando yepamusoro vanogona kutungamira kune isina kunaka uye inodhura mibairo.

HPLC vials inowanikwa kubva kune dzinoverengeka-dzinozivikanwa kwazvo mhando, imwe neimwe iyo ine zvazvinazvo zvine hungwaru uye zvakanaka. Mvura, Agilent Thermo Fishera uye shimadzu ndeimwe yemhando dzinozivikanwa kwazvo. Usati waita kutenga, zvakakosha kuenzanisa uye kutsvagurudza marudzi akasiyana.

Vii. Mhedziso

Kusarudza kurudyiHplc VialNekuti yako chromatography system yakakosha kuwana mibairo chaiyo uye yakavimbika. Kana uchisarudza HPLC vials, zvinhu zvakakosha zvinosanganisira izvo zvoumbozha uye kugadzirwa kweiyo chromatography system uye mhando dzemhando, pamwe nemhando dzekudzora mhando. Vanyori vanogona kuwana mibairo yakakwana uye nekutevera maitiro akanakisa pakubata uye kuunganidza Hplc vials, kusanganisira kunyengedza mwenje, kunyorova nekunyanyisa kupisa uye kuongorora vials usati washandiswa.

Kune akawanda HPLC vial sarudzo uye mabhurawuti anowanikwa. Izvo zvakakosha kuenzanisa uye kutsvagisa iyo yakasiyana mabhureki kuitira kuti isarudze iyo yakakodzera sarudzo yezvido zvako zvekuongorora. Vanoongorora vanogona kuwana mhedzisiro yakavimbika uye inosarudzika nekusarudza inoenderana, yakakwirira-mhando vials, uye kutevera kuchengetedzwa kwakakodzera uye kubata maitiro.