Is UHPLC always better than HPLC?

UHPLC offers higher resolution and faster analysis, but it comes with greater cost and stricter requirements—so “better” depends on your specific application.

Can I use the same columns for HPLC and UHPLC?

Typically not. UHPLC columns are optimized for higher pressure and smaller particles, and standard HPLC columns may not perform optimally or safely in UHPLC systems.

What key consumables should I upgrade for UHPLC?

Focus on low-adsorption vials, ultra-clean filters and injection systems with minimal dead volume, to maintain peak shape and reproducibility in UHPLC.

Qeybaha

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Weydiin

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HPLC ama Uhprc? Sida loo doorto farsamooyinka chromatoraphy ee saxda ah ee shaybaarkaaga

Oktoobar 31-keeda, 2025

Tilmaamaha: Hplc vs uhplc Chromatography dareere ah Kimistariga falanqaynta Chromaloongrographylowglow Aijiren wuxuu siiyaa is-weydaarsiga Tiirarka hplc Uhprc safka Falanqaynta shaybaarka Isbarbardhiga isbarbardhiga Uhplc vials

Waa maxay hplc?

Cudurka loo yaqaan 'chromacographiographiographiographicy' oo sareeya ayaa ka buuxsamay sadarka ka buuxay walxaha waaweyn (caadiyan 3-5 μm), oo ka shaqeeya dhexdhexaad ah (badiyaa ilaa ~ 400-600), waxaana si weyn looga isticmaalaa falanqaynta joogtada ah ee dawooyinka, baaritaanka cuntada iyo shaybaarka biyaha. Waa la isku halleyn karo, si fiican loo ansaxiyay, oo la jaan qaadaya shaqooyin badan oo jira oo jira.

Waa maxay Uhprc?

Ultra-High-Him-Hit-Section Fareadeeya Khible-ka-yar ee 'chromatography' Faa'iidooyinka waxaa ka mid ah waqtiyada dhaqsaha badan, hufnaanta sare iyo xallinta xalka. Sheyga sheybaarka wax ka qabashada isku dhafka isku dhafka ah, raadinta falanqaynta ama u baahan wax soo saar weyn, Uhprc waa ikhtiyaar adag.

Kala duwanaanshaha muhiimka ah ee u dhexeeya HPLC iyo Uhplc

Darada	Hprc	Uhplc
Cabbirka walxaha	3-5 μm	≤ 2 μm
Cadaadiska caadiga ah	Ilaa ~ 400-600 bar	Ilaa ~ 1000-1500 bar
Xawaare & wax soo saar	Dhexaad ah	Sare ah
Qaalkarka	Caadi ah	Kor u qaaday
Qiimaha qalabka	Ka hoose	Sare

Goorma ayaa la adeegsadaa hplc

HPLC waa mid fiican marka matrix-kaaga muunad lagu maareyn karo, baahiyaha wax soo saarka waa dhexdhexaad, caqabadaha miisaaniyada ayaa jira, oo aad mudnaanta siisaa, hababka xasilloonida ah. Haddii dalabkaagu ku lug leeyahay heerka caadiga ah ee molecule ee molecule assays, qa \ / Qc.

Goorma ayaa la adeegsadaa Uhplc

Haddii aad wax ka qabato saamiyo aad u adag (E.G. Shaybaarta dabiiciga ah, metabonomics), u baahan deg-deg ah, xasaasiyad sare ama kala-goyn shahwad, ka dib Uhprc ayaa laga yaabaa inuu noqdo maalgashiga wanaagsan. Ka digtoonow, in kastoo uhplc ay u baahan tahay xaalado nidaam xasaasi ah oo aad u adag (mugga dhimatay, bamka waxqabadka sare, suuxdin la fidiyo) iyo bilow weyn.

Qandaraaslaha ayaa muhiim ah

Tallaabada ka socota HPLC ilaa UHPLC waxay sidoo kale saameyn ku leedahay xulashada tabarucayaasha. Xulashada vials tayo sare leh, autosampler-ka, miirayaasha, iyo tiirarka, iyo tiirarka ayaa wax badan ka sii badan u-wareejinta UhplC-ka darsashada durugsan. Tusaale ahaan, sheybaarka isticmaalaya Uhplc waa inuu tixgeliyaa vials-ka-adkeynta-adkeynta, miirayaasha cuntada ee loo yaqaan 'ultra-nadiif nadiif ah' iyo qalabka lagu saleeyo ee mudiska lagu sameeyo si loo ilaaliyo waxqabadka.

Maxaad u doorataa culimada 'isna'

Teknolojiyadda Zhejiang Aijiing Aijiren Co. Qurbaneheena waxaa ka mid ah vials la caddeeyay, Sep septa, nadiifi miirayaasha iyo oem \ / odm xalka caadada. Adigoo adeegsanaya Aijirn Stubles, shaybaarku waxay helayaan waxqabad la isku halleyn karo, hoos udhaca faddaraynta asalka iyo hab ka wanaagsan taranka.

HPLC iyo Uhprc labadaba waxay leeyihiin meelahooda shaybaarada casriga ah. HPLC waxay weli tahay mid wax ku ool ah oo xoog badan falanqeeyeyaal badan oo caadi ah, halka Uhplc ay bixiso xawaare sare iyo xalin xalaal ah xaalado aad u baahan. Furaha ayaa la jaan qaadaya qalabkaaga, qulqulatada shaqada, cunista, iyo miisaaniyadda yoolalkaaga dootyada.

Su'aalaha badanaa la isweydiiyo (FAQ)

Uhplc had iyo jeer ma ka fiican yahay HPLC?

Uhprc waxay bixisaa xalka sare iyo falanqaynta degdega ah, laakiin waxay la timaadaa kharash weyn iyo shuruudo adag - sidaa "si ka wanaagsan" waxay kuxirantahay dalabkaaga gaarka ah.

Ma u isticmaali karaa isla tiirarka sita hplc iyo Uhprc?

Caadi ahaan maahan. Safka uhprc safka hoose waxaa lagu hagaajinayaa cadaadis sareeya iyo walxaha yaryar, iyo tiirarka caadiga ah ee HPLC-ga ma u fulin karaan si fiican ama si nabdoon oo ku jira nidaamyada UhppC.

Waa maxay furaha muddada furaha ah ee aan u sahor u leeyahay uhplc?

Xoogga saar finanka xayeysiinta-hoose, miirayaasha cagta-nadiifka ah iyo nidaamyada duritaanka ee mugga ugu weyn, si loo ilaaliyo qaabka ugu sarreeya iyo taranka ee UHPLC.

Hore:

Tilmaamaha muunad hplc ah - Tilmaanta talaabada-talaabada

Xiga:

Ku noqo liiska

HPLC ama Uhprc? Sida loo doorto farsamooyinka chromatoraphy ee saxda ah ee shaybaarkaaga

Waa maxay hplc?

Waa maxay Uhprc?

Cabbirka walxaha

3-5 μm

≤ 2 μm

Cadaadiska caadiga ah

Ilaa ~ 400-600 bar

Ilaa ~ 1000-1500 bar

Xawaare & wax soo saar

Dhexaad ah

Sare ah

Qaalkarka

Caadi ah

Kor u qaaday

Qiimaha qalabka

Ka hoose

Sare