Laga soo bilaabo biyaha xoogga aadka ah ee loo yaqaan 'acidic' si acidic-ka si loo isticmaalo dawooyinka lipopophophilic-ka, xulashada saafiga khaldan ee muunadda ah waxay ku soo baxaan soosaarista ama kicinta saqafka pintomka. Tilmaamahan wuxuu ku faafiyaa xadka waxqabadka ee soodhada-lime iyo dhalada dabiiciga ah waxayna bixisaa istiraatiijiyado vial-iyo-sept ah

Shuruudahaaga Shaybaarkaaga & Codsigaaga

Warshad	Muunad caadi ah	Baahiyaha weelka muhiimka ah
Deegaan	Biyaha aashitada (ph 1-3)	Iska caabbinta jiirka; Liiska birta ee Zero
Pharma r & d	APIS ayaa ku milmay acetonitrile	Iska caabin dabiici ah; Xayeysiinta Hoose
Tijaabada cuntada	Qalabka caagga ah ee saliidda cuntada	Iswaafajinta liidata; Ilaalin fudud

Isbarbardhiga Waxqabadka Qalabka

Wax	Sifooyinka muhiimka ah	Isku-darka kiimikada	Qiimaha (€ € vial)	Adeegso kiisaska
Galaas soodhada-lime	Ugu yaraan qaali	ph 6-8 kaliya	~0.5	Barista shaybaarka; Biyo-gaaban
Hoos udhaca	Caabbinta asaasiga asaasiga ah (ph ≥4)	Maaha HF-ama saldhigga adag ee u adkaysta	★★☆☆☆	Biyaha caadiga ah ee HPLC
Dhex-dhexaad	ph 0.5-10; AutosLappeble At 120 ° C	FARSAMADA PH; kuleylka-jeermiska	★★★☆☆	Pharma Qc; Muunado noolaha ah
Sare-Borosalecate	Dheeraad ah PH 0-14; si dhakhso ah u deggan	Dhammaan aashitada \ / saldhigyada; 500 ° C Awoodda	★★★★☆	Raad raac \ / falanqaynta aashitada ee aashitada

FIIRO GAAR AH: Gawaarida Dhex-dhexaadka ah ee-Borosalistate-ka USP \ / Ex Heerarka Dhaqadaha Dacwada. CTE-BOSIALIT CTE ≈3.3 × 10⁻⁶ \ / k vs. 9 × 10⁻⁶ \ / k oo loogu talagalay galaaska caadiga ah.

Xulashada Sep-ka saxda ah

Nooca Septum	Burburinta nolosha	Isku-darka kiimikada	Dib u dhac	Ku habboon
Ptfe \ / silicone-ka silicone	15-20 marqaati	Aashitada, saldhigyada, dareeraha dhexdhexaad ah	DMF ayaa khiyaanayn kara	HPLC Autosampler ayaa ordaya
PTFE saafi ah	Hal adeegsi	Dhammaan dareeraha dabiiciga ah	Xadhiga liita	GC-MS MALOGENENED Assayds
Silicone-ka horay loo dhajiyay	30+ duuliyaha	Aashitada daciifka ah \ / saldhigyada (ph 3-10)	Barar acetone	Shaashadda sare ee wax soo saarka

Talooyinka Final & Septa ee muunad

Nooca muunad	Vial lagu taliyay	Xulashada Septus	Garsooyinka si looga fogaado
Biyaha wasakhda ah (PH 1.5)	Sare-Borosaile Cas oo cad	White PtFe \ / silicone-ka silicone	Weligaa ha u isticmaalin liin-lime (kala diri) 24 saac
Dawooyinka daroogada ee acetonitrile	Dhex-dhexaadka dhexdhexaadka ah	Pluf PTFE PTFE	Horyaalka Hoose-Borosailes ah oo loo yaqaan 'Peak'
Faa'iidooyinka saliida ee khudradda	Amber High-Borosalistacate	Silicone-ka horay loo dhajiyay	Galaas cad ayaa u baahan ilaalin fudud
Barista wax barista (dhexdhexaad ah)	Dharka-lime vial	Standard Silicone	Kaliya sanadaha muddada-gaaban, oo ah

Daraasadda kiiska: Qaabka loo habeeyo

Setup asalka ah:
- Muunad: API gudaheeda 0.1% Foomka Acid \ / acetonitrile (1: 1)
- Weelka: Lower-Porosaileit Vial + Heerka Setty ee Silicone
Arrimaha:
- Horudhac aan la garanayn ayaa ka soo muuqday mudista # 3 (shaashadda silikone)
- Maaddada API-da ayaa ku dhacday 12% ka dib 72 H (Qalabka dhalada)
Xalka:
- Kor u qaad ilaa vials-ga galka-galka
- U beddelo ptfe \ / silicone closite septa (pre-pre-pre-presited 5 ×)
Natiijooyinka:
- Phantom Peaks ayaa la tirtiray
- Kala duwanaanshaha Imtixaanka Xasiloonida <0.8%

Kale Takecaano

Aashitada adag → sare-borosaile
U hoggaansanaanta Phoncha → Dhexdhexaad-Borosalistate
Muunado-gaaban oo ka-hortagga ah → soodhada-lime
Septa Chemistry> Nolosha nolosha

Tixraacyada Xogta:

USP <660> Imtixaanada weelka dhalada
CMA-Xaqiijinta Qoraalka Laylaan

Tilmaamahan, la dhig vial-gaaga iyo septam-kaaga si aad u hesho kimistariga-ka fogaada dib-u-shaqeynta qaaliga ah oo hubi natiijooyinka soo saarida ee guud ahaan falanqaynta deegaanka iyo dawooyinka.

Hore:

Waa maxay farqiga u dhexeeya GC-Ms iyo GC-Ms \ / MS?

Xiga:

Ku noqo liiska

Tilmaamaha: Lings vials GC-MS muunad Prep Dhacdooyinka dhalada ee sare-galka ah Dhex-dhexaadka Dhex-dhexaadka ah Ptfe \ / silicone septa

Su'aalo weydiinta