Nadiifinta vials-ka madaxa & dib-u-isticmaalka: Shaqo-gudowga, isbarbardhiga khasabka ah ee lagu tuuri karo vs. vials dib loo isticmaali karo

1. Hordhac

Vials vials-sida caadiga ah ka samaysan dhalada dabiiciga ah - waxaa si weyn looga isticmaalaa GC iyo GC-Ms si ay u falanqeeyaan xeryahooda kacsan ee lakabka madax-bannaanida. Dib-u-sheeg iyaga ka dib nadiifinta saxda ah iyo ka-boodbooda oo aan yarayn oo keliya waxay yareysaa kharashaadka aan caadiga ahayn laakiin sidoo kale waxay taageertaa waafajinta shaybaarka.

2. Noocyada Val & codsiyada ku habboon

• Vials-ka-daboolka-ka-riix (dusha sare): Way fududahay in la furo \ / Dharso, oo la jaan qaada inta badan Autosamplers, oo ku habboon isticmaal soo noqnoqda oo ku saabsan falanqaynta jidadka ee joogtada ah.

• Rinp-koofiyadaha vials (aluminium rinp lid + Septa): Bixi xirmo dhirta, inta badan isticmaal keliya maxaa yeelay cashuurta ayaa sababa isqaba. Waxaa laga doorbiday cadaadis sare, awood sare leh, ama falanqeyn xagal ah oo xasaasi ah (E.G. FARSAMADA, CUNTADA, PRECTOUTutical).

3. Nadiifinta tuurista & haraaga haraaga ah

Diyaarinta:

• Muunad harsan hadhaaga.

• Qulqulka ku qabooji gudaha burush ama xoq.

• Ka saar oo nadiifi CAP iyo Septa si gooni gooni ah.
  
  

Nidaamyada nadiifinta tallaabada badan ee caadiga ah (laga soo qaatay borotokoolka shaybaarka ee la daabacay):

Habka a (guud ahaan haraaga dabiiciga ah)

1. Ku qoyso 95% ethanol

2. Ultrasonic nadiif ah laba jeer

3. Ku mayr laba jeer biyo fog

4. Foornada-qallalan ~ 110 ° C 1-2 saacadood

Habka B (Biyaha ku saleysan \ / faddarayn yar)

1. Ku mayr biyo dhabarka si isdaba joog ah

2. Ultrasonic oo ku jirta biyo aan laga adkaatay (15min × 2)

3. Ku qoy ethydrodrodrodrodrods ethanol ka dibna hawo qalalan

Habka c (methanol-culeys)

1. Methanol sok + 20min ultrasonic

2. Biyaha ultrasonic (20min)

3. Si fiican u qalaji

Habka D (oo xoog leh oo nadiif ah oo nadiif ah faddaraynta culus)

1. Cabbisyada acid: sulfuric acid + potassium dichromate song → biyo raaci

2. Aalkolaadda khamriga ee caafimaadka ≥4h + 30min + 30inttrasound

3. Biyaha ultrasound mayl

Habka e (oxidative + Qiimaha degdega ah)

1. 24H ku qoy xalka xalka dichromate

2. Biyo-biyoodka ee loo yaqaan 'ultrasonic' oo biyo raacaan (× 3)

3. Methanol mayr → hawo qalalan

4. Had iyo jeer ku beddel Septa \ / galaaska galka markii la adeegsanayo

4. Daaweynta ka-hortagga Daweynta

Si loo yareeyo haraaga ku-meel-gaarka ah ee adsorbed-ka ah:

• Kuleylka kululeedka vials ee foornada (110-150 ° C) 1-2 saacadood.

• Ikhtiyaar ahaan u nadiifi gaaska intert gaaska ama wareegga faaruqinta.

• Waxay kordhiyaan isugeynta inta lagu gudajiro infakshanka GC madaxweynaha ee GC madaxa si ay u caawiyaan haraaga haraaga ah.

Tallaabooyinkan ayaa yareynaya "cirfiidhada naxaasta" iyo sanqadha asalka ah ee ku yaal falanqaynta GC.

5. Xaqiijinta & Xakamaynta Tayada

• Tijaabinta haraaga ah: U isticmaal falanqaynta TUCC ama ku samee cirbado madhan ah oo ku sii socda GC-HS oo isbarbar dhig asalka ugu sarreeya kuwa ka socda finanka cusub si loo hubiyo in aan la arkin wax aan la fileynin.
  
  

• Halbeegyada Xaqiijinta Qaabka: Sax ahaanta (dib-u-soo-celinta), sicir-sare, sicirka soo kabashada (iyada oo loo marayo heerarka lafijka), xadidaada xuduudaha - dhammaantoodna muhiim u ah in la xaqiijiyo in vials nadiif ah oo u dhigma kuwa cusub.

• Nidaamka Qc: La soco tiro kasta oo vial ah oo dib u isticmaal ballacyada. Dhaqangelinta xadka (E.G. 3-5 adeegsiga). Joogtaynta diiwaannada nadiifinta, mudhooyinka maran ee xilli-dhaaminta, iyo kormeerka goos goosyada.
  
  

6. Dib u isticmaal nolosha nolosha iyo khataraha

• Ficil ahaan, vials Porosaileit waa la si badbaado leh dib loogu isticmaali karaaqiyaastii 3-5 jeerKa dib markii la ansaxiyay nadaafadda iyo habraaca QC.
  
  

• Khataraha dib-u-isticmaalka:

  • Fasaxa-ka-dheganaanta → Ghost Peaks ama gaari (gaar ahaan farta hordhaca ah)

  • Cilladaha Sept-ka ama daadashada ee tayaarka ah ee shaabadda

  • Dhaawacyada dusha sare ee dhalada

  • Isbeddellada ku jirta nadaafadda udhaxeysa vials iyo dufcaddu waxay horseed u keentaa taranka liita
    
    
    

7. Isbarbardhiga kharashka & halista: Isticmaalka hal-dib loo isticmaali karo

Shaybaar badan, qarashka qarsoon (shaqada, QC, ee loo yaqaan 'The Shaqooyin', Tilmaamaha, Fursadaha Fashilmay, oo ay sabab u tahay faddaraynta) ee ay ka hortagaan kaydinta - gaar ahaan dareenka heerka iyo heerka raad-raaca ayaa ah shuruudaha.

8. Talo soo jeedinta & waxqabadka ugu wanaagsan

• Xulo hanaan nadiifin oo ku saleysan muunad muunad wasakhaysan; Adeegso borotokoolka adag ee oktorol-ka oo keliya markii loo baahdo.

• Had iyo jeer beddel Septa; Dib-u-dhiska Koofiyadaha \ / Septa waxay horseedaa daadad iyo cillad.

• Hirgal Sps si loo kala sooco dhalada nadiifka ah ee vs, raadraaca tirinta dib u eegista, iyo diiwaannada fayadhowrka.

• Si joogto ah u xaqiijiska ah ee muddada-casharka iyo galka GC madhan; Tuur dhalooyinka Mar alla markii QC ay guuldareysato ama ka dib marxaladaha isticmaalka marinka marinka.

• Falanqaynta sare ama falanqaynta raad-raacyada (E.G. PHONA, FARSAMO), UGU DANBEEYA KELIYA SAXIIRKA KELIYA-ISTICMAALO KELIYA IYO DHAQANKA.

• Tababar shaqaalaha si loo hubiyo hawlgallada caadiga ah iyo aaminka ah, oo ay ku jiraan isticmaalka PPE marka la maareeyo aashitada iyo dareeraha.
  
  

Kooban

• Faahfaahin faahfaahsan, nadiifinta badan-iyo-ka-nadiifinta badan ee lagu daro kuleylka kuleylka ayaa laga yaabaa inuu dib u soo celiyo dhalada dhalada dhalada dib loo isticmaali karo dhowr jeer oo aan tanaasulaad weyn lahayn.

• Si kastaba ha noqotee, habka ugu celcelisnada lagu soo celiyo waxay soo bandhigayaan kakanaanta: shaqada, qalabka, iyo waqtiga Qc ee Qc ​​waxay ka badnaan karaan kharashyada-gaar ahaaneed ee raadinta, nidaaminta, ama shaybaarka saxda ah, ama shaybaarka.

• Hirgelinta geedi socodka cad ee cad ee loo yaqaan 'Spops Spops' iyo Nidaamyada Anfaqaynta Si loo badbaadiyo Isu-dheellitirka Dhaqaalaha, Saamaynta Deegaanka, iyo Tayada Doodaca, iyo Tayada Doodacayaasha iyadoo dib loo adeegsanayo ~ 3-5 wareegga inta ay kormeerayaan halista wax ku ool ah.