I-chromatography yindlela esetyenziswe ngokubanzi kwilebhu ukwahlulahlula kunye nokuhlalutya amanzi. Elinye lamacandelo aphambili e-vial yeChromatography yiSeptum. I-septum ngumqobo otywina i-vial kwaye ivumela isampulu ukuba igcinwe kwi-chromatograph. Yezinto ezahlukeneyo ze-septum ezikhoyo,I-ptfe (iPolytetrafluoroOuthune)zikhetheke kakhulu ngokuchasene nekhemikhali kunye nokungabikho. Nangona kunjalo, ukukhetha uhlobo olufanelekileyo lwe-septum equbintiweyo ye-PTPU-kufuna ingqalelo kwizinto ezininzi ukuqinisekisa ukusebenza kakuhle kunye nokuhambelana neemfuno zakho zeChromatographic. Amanye amanqaku abalulekileyo ukugcina iingqondo zidweliswe apha ngezantsi.

1. Ukuhambelana kwamachili

Ngaphambi kokukhetha i-septum edityanisiweyo ye-ptfewel, kubalulekile ukuba uchonge iikhemikhali kunye nezicoci ezibandakanya uhlalutyo lweChromatographic. I-PTUSE yaziwa ngokuba kukunganyangeki kweekhemikhali zekhemikhali, kuyenza ilungele uluhlu olubanzi lwezicelo. Inganyangeki kwii-acids, iziseko, izinyibilikisi zezinto eziphilayo, kunye nezinye iichemicals ezininzi, kwaye ii-sepmems zihlala zisebenza kwaye aziyi kunxibelelana nesampulu okanye ichaphazela kakubi uhlalutyo. Nangona kunjalo, kubalulekile ukuba iqinisekise ukuhambelana ngokuthe ngqo kunye neekhemikhali ezisetyenziselwa ilabhoratri ukuthintela iingxaki kunye nongcoliseko.

2. Uzinzo lobushushu

Iindlela ze-chromatographic zihlala zibandakanya ukuvezwa kumaqondo obushushu aphezulu, ngakumbi kwi-chromatography yegesi (i-GC) kunye neqondo eliphezulu le-chromatography (i-HPPC). Umgangatho ophezuluI-PTFE-cophred septumszenzelwe ukumelana namaqondo obushushu ngaphandle kokuthambisa, ekuqinisekiseni ukusebenza okungaguqukiyo kunye nokutywina okuqinisekileyo kwe-vials. Kubalulekile ukukhangela inkcazo yobume bamaqondo obushushu anikezelwe ngumenzi ukuqinisekisa ukuba iSeptum inokusingatha ubushushu obuveliswe ngexesha lenkqubo yeChromatographic.

I-3. Ubungqingqwa kunye nokuqina

Ubungqingqwa be-PTFI COADS kwi-septum idlala indima ebalulekileyo kubundisiso kunye nobomi obude be-septum. Ukudibana okuthe kratya kuphucula ukungcungcutheka nokunciphisa umngcipheko womonakalo kwi-septum ngexesha lenaliti ezininzi. Oku kubaluleke kakhulu kwiilabhoratri eziphezulu zokufaka apho i-Vials isetyenzisiwe kwaye isampuludliwe rhoqo. Ukudibana okutshatileyo kwandiswa ubomi baseSeptum, ukunciphisa isidingo sokutshintshwa rhoqo nokuqinisekisa ukusebenza okungaguqukiyo ngokuhamba kwexesha.

Ujonge ukwanda ulwazi lwakho kwi-HPPC ye-vial vial sepga? Dive kweli nqaku ukuze ufumane ulwazi oluneenkcukacha kunye nolwazi olubalulekileyo ekukhetheni i-septa elungileyo yohlalutyo lwakho:Yintoni i-vial vial sepga?

I-4. Inaliti yokungena kunye neempawu zokuziqhelanisa

Ukungena ngenaliti ngokufanelekileyo kunengxaki yesampulu egudileyo nechanileyo kwinaliti kwi-chromatography. I-PTFE-compt enamaplanga kufuneka ivumele ukungena ngokulula ngaphandle kokungena okanye ukushiya inkunkuma enokungcolisa isampulu okanye iphazamisane nohlalutyo. Ukongeza, ukugcina ingqibelelo ye-Vial kunye nokuthintela ukwanda okanye ukuvuza kwesampulu, i-septum kufuneka ibonise iipropathi zokuziqhelanisa emva kokuba inaliti isusiwe. Oku kuqinisekisa iziphumo ezinokuthenjwa kwaye zibuyisiwe kwiinaliti ezininzi.

5. Ukuqwalaselwa okukhethekileyo

Kuxhomekeka kwisicelo seChromatographic, kusenokuba kusekho iimfuno ezithile ezichaphazela ukhetho lweI-ptfe-idityaniswe kwi-septum. Umzekelo, kwi-chromatography yegesi (i-GC), apho imiqathango eguqukayo iyahlalutywa, kubalulekile ukukhetha i-septum enciphisa ilahleko yesampulu ngenxa yokuphuma. Ngokwahlukileyo koko, kuhlalutyo olubuthathaka obuthathaka efuna amanqanaba asezantsi e-Ultra efuna izinto eziphantsi, ii-sepmem ezinesithambiso esincinci kwaye ukuqhutywa ngenyameko kuyacetyiswa ukunqanda ukuphazamiseka kweziphumo zesampulu. Xa ukhetha iSeptum, cinga ngeemfuno ezithile zendlela yeChromatographic kunye nohlobo lwesampulu.

Unomdla wokufunda malunga nokusetyenziswa kwe-15 yezicelo ze-vialsgraphys? Phonononga eli nqaku ukuze ufumane ulwazi olunzulu kunye noshwankathelo olubanzi lokusebenzisa kwabo izinto ezahlukeneyo:I-15 izicelo ze-vialsgraphys kwimimandla eyahlukeneyo

I-6. Uhlobo lwe-vial kunye nobungakanani

I-PTFE-i-PTFE-enamaplanga enamagqabi ayafumaneka kwiintlobo ngeentlobo zesayizi kunye noyilo lokulinganaI-chialsgraphy ye-chromatography. Ukufezekisa ukutywina ngokuchanekileyo kunye nokuthintela iingxaki ezinje ngokuvuza okanye ukutywinwa ngokungafanelekanga, kubalulekile ukuqinisekisa ukuba iSeptum iyahambelana nohlobo lwe-vial esetyenzisiweyo. Jonga iinkcazo ezibonelelwe ngumenzi ukukhetha ubungakanani be-septum echanekileyo, isitayile okanye oqinileyo okanye oqinileyo, njl. Njl.

7. Udumo lomvelisi kunye nokuqinisekiswa komgangatho

Xa ukhetha i-AI-ptfe-idityaniswe kwi-septum, qwalasela intetho edumileyo yomenzi kunye neendlela zokuqinisekisa umgangatho. Abavelisi abadumileyo balandela imilinganiselo esemgangathweni kwaye banokubonelela ngezatifikethi kunye nokuvunywa okuqinisekisa umgangatho weSeptum kunye nokufaneleka kwezicelo ze-chromatographic. Jonga iimveliso kubathengisi abadumileyo ezihlangabezana nemigangatho yemigangatho kwaye banengxelo engqiniweyo yokuvelisa ii-septom ezinokuthenjwa ezifaka isandla ekuchanekeni, ukuzala, kunye nokusebenza kakuhle kohlalutyo lweChromatographic.

Ukuqukumbela, ukukhetha uhlobo olufanelekileyo lwe-septum ye-PTROAMUM ye-vial ye-chromatography kufuna ukuba kuhlolwe izinto ezifana nezakhiwo ezifana nezakhiwo, ukuzinza kweqondo lobushushu, ukuqinisekiswa kohlobo lwe-Vial, kunye nokuqinisekiswa komgangatho we-vial, kunye nokuqinisekiswa komgangatho. Oku kulandelayo zezinto ezifuna ukuvavanywa. Oku kulandelayo sisishwankathelo esifutshane sezinto ekufuneka ziqwalaselwe kuvandlakane nokuvavanywa kwezi zinto kwaye bakhetha i-septum esemgangathweni evela kumvelisi oqinisekileyo, ukuveliswa kwenkqubo yakho.

Ukuqonda okubanzi kwePTFO / Isiliki Septa, singena kweli nqaku lifundisayo. Fumana ulwazi oluxabisekileyo kunye nobungcali kwiSepta !:Yonke into oyifunayo

Ngaphambili:

Ukuphucula isampulu yesampula: Isikhokelo esibanzi sokwenza umsebenzi ofanelekileyo kunye neefilitha zokusebenza eziphezulu

Okulandelayo:

Imiba esemgangathweni kwi-vialsgraphycy: oonobangela kunye neziphumo