Ukukhetha i-vials efanelekileyo ye-autosampler isitshixo. Ukuzisebenzisa kakuhle kunjalo, nazo. Bagqiba ukuba uhlalutyo oluthembekileyo nolwachanekileyo. Ivolumu ye-Vic inokuba negalelo ingqibelelo yesampulu. Ikwachaphazela ukuchaneka kwedatha. Eli nqaku libonisa ubuchule bokuxhasa i-Holc AutoManter Voloko ye-vial viali. Injongo kukusika ilahleko yesampulu kwaye ugcine uhlalutyo luchanekile.

Ukuqonda ivolumu eqhelekileyo

Kukho ubungakanani obuqhelekileyo be-HPLC ye-vials ekhoyo, njenge-1.5-2 ml, 4 ml, kunye ne-150-300 μ L. Eyona nto isetyenziswa kakhulu kwaye ikwazi ukuhlangabezana neemfuno zovavanyo lwemihla ngemihla zii-1.5-2 ml isampulu ye-vials. Xa iisampulu zifuna ivolumu ingaphezulu kwe-3 ml, sisebenzisa i-4 ml yezikhongozeli. Ezi vils ezinkulu zinokubamba i-microfats ngaphakathi kubo. Ezi zisetyenziselwa imiqulu emincinci eninzi kakhulu, phakathi kwe-150 ne-300 μl.

Ukwazi iimpawu kunye nokusetyenziswa ngokufanelekileyo kwezi vils ze-autosampler vial. Ukukhetha ubungakanani be-Vial efanelekileyo kubaluleke kakhulu. Kufuneka ihambelane nevolumu yesampulu. Oku kunceda ukunciphisa ilahleko yesampulu kwaye ugcine isampulu yealth ngexesha lohlalutyo.

Funa ukwazi ngakumbi malunga ne-HPPC ye-HPPC, nceda ujonge eli nqaku Ukufakwa kwe-HPLC: ukuphucula inyani kunye nempazamo yesampulu

Ungayikhetha njani ngokwesampulu

Ukukhetha ubungakanani be-Vial efanelekileyo kubalulekile ukunciphisa ilahleko yesampulu. Njengommiselo oqhelekileyo, ivolumu yesampulu kufuneka ilingane ngokusondeleyo amandla e-vial. Sebenzisa i-vial enkulu kakhulu ukuba isampulu inokwakha kakhulu intloko. Oku kukhulisa umngcipheko wokuvela kwamanzi, ngakumbi kubahlali abaguqukayo. Kwelinye icala, ukukhetha i-vial engaphantsi kakhulu kunokukhokelela kwisampulwa kunye nelahleko.

1.assess ivolumu yesampula:

Ukulinganisa okanye ukuqikelela ivolumu yesampulu yakho ye-HPPC.

Cinga ngotshintsho lwevolumu. Olu tshintsho lunokubangelwa kukuncamathisi, ukuphindaphinda, okanye amanye amanyathelo okulungiselela.

2.Make ivolumu yesampulu kwi-SIFRIP eqhelekileyo:

Kwimifanekiso emincinci yesampulu (i-150-300 μl), i-Microfants ifakwe ngaphakathi kwi-vial vial vial (E.G., 2 ml) iyacetyiswa.

Kwimithamo yesandla ephakathi (i-0.5-2 ML), imigangatho ye-1.5-2 Ml Vials ihlala ilukhetho olufanelekileyo.

Kwimithamo enkulu yesampulu (ukuya kuthi ga kwi-3 ml), i-4 ml vials inokulunga kakhulu.

3.Conicles I-RECE:

Injongo yokunciphisa i-Serpace (ivolumu engenanto) ngaphakathi kwe-vial.

I-REAPECE egqithisileyo inokunyusa umngcipheko wesampulu yokuphuma kwesampulu, ngakumbi kubahlali abaguqukayo.

Ivolumu yesampulu kufuneka inxibelelane kufutshane ne-vial septum okanye i-cap yokwenza itywina elinzima.

4.Uyimfuneko kwimfuno yokufaka:

Kwimithamo encinci yesampulu, i-Micro faka kwi-vial enkulu inokunciphisa indawo entloko. Oku kuseta kukwanciphisa ukubonakaliswa kwesampulu.

Ukufaka i-Micro faka kwimiqulu eyahlukeneyo, enje nge-150-300 μl. Banokulingana nobukhulu beesampulu ezahlukeneyo.

Ufuna ukwazi ukuba ukhethe indlela yokukhetha uhlobo olufanelekileyo lwe-Micro-faka i-vials yakho ye-chromatography Ungalukhetha njani uhlobo olufanelekileyo lwe-Micro-fin ye-vials yakho ye-chromatography yakho

5.Ukuqinisekisa kwaye uqinisekise:

Ukuqhuba uvavanyo lwangaphambili usebenzisa ubungakanani be-vial ekhethiweyo kunye nakuphi na ukufaka.

Ukubeka iliso kwisampula, uzinzo, kunye nazo naziphi na iimpawu zokuphuma komgangatho okanye ukonakala.

Lungisa ubungakanani be-vial. Okanye, sebenzisa ukufakwa kwempahla njengoko kufuneka. Oku kuyakwenza ukuba ukhathalelo lwesampulu kunye nokulondolozwa.

Landela la manyathelo. Ungafumana ubungakanani be-HPLC ye-HPLC yevolumu yakho yesampulu. Iya kunceda kwakhona ukunciphisa ilahleko yesampulu. Khumbula ukuqwalasela wonke umsebenzi wendlela yokuqhuba. Kwakhona, qwalasela naziphi na iimfuno zohlalutyo olukhethekileyo xa ukhetha.

Ufuna ukwazi ukuba ukhethe indlela yokukhetha i-cap ye-chromatography ye-chromatography yakho, nceda ujonge eli nqaku Ungalukhetha njani uhlobo olufanelekileyo lokufaka i-vials yakho ye-chromatography

Ukunciphisa i-espace kunye nokuvezwa

Eyona nto ibalulekileyo kukukwazi ukukhetha ivolumu ye-voin elungele inani leesampulu. Oku kunceda ukunciphisa indawo entloko kwaye kunciphise indawo yomphezulu evezwe kwindawo engqongileyo. Ngokuthelekisa ubungakanani ngononophelo ubungakanani besampulu, unokunciphisa ilahleko yesampulu ngenxa yokuphuma okanye ukuchitheka.

Ukuba isampulu yesampulu incinci kwaye i-vial enkulu isetyenziswa, i-visi enkulu egqithisileyo okanye ivolumu engenanto iya kwenziwa kwi-vial, eya kukhawulezisa isampula kwaye ithobe ukuthembeka kwesampulu. Ukuthomalalisa lo mngcipheko, owona mngcipheko mncinci wokuvala onokufumana ivolumu yesampulu ngelixa uvumela ukutywinwa ngokufanelekileyo kunye nokuphathwa okufanelekileyo kufuneka kusetyenziswe. Ukuqinisekisa ukuba isampulu inxibelelana ngokuthe ngqo ne-vial septum okanye i-cap inceda ukudala itywina eliqaqambileyo kwaye linciphise indawo eboniswe kuyo.

Ukufakwa kwe-vial vial

Ukufakwa kwe-Vial, ngakumbi ukufaka i-Micro, kungabonelela ngomaleko ongezelelweyo wokukhusela imiqulu emincinci yesampula. Oku kufaka i-faks ifanelekile ngaphakathi kwi-vis enkulu ye-Vial, ngokunciphisa ngokufanelekileyo isicwangciso esikhoyo kunye nokunciphisa ukubonakaliswa kwesampulu. Umzekelo, unokuqinisekisa ukuthembeka kwemithamo encinci yesampulu ngokufaka i-1.5-20 vials.

Ukuqukumbela

Ukukhetha ivolumu ye-vial ye-vial ye-vila efanelekileyo kakhulu ukugcina ingqibelelo yohlalutyo lwesampula. Kweli nqaku, safunda malunga nemiqolo eqhelekileyo ye-Vial, indlela yokukhetha i-vial vial, kwaye kutheni ibalulekile. Siya kukhetha i-vial efanelekileyo yokunciphisa ilahleko yesampulu kwaye sinike idatha ethembekileyo.

Hlalani kwizicelo ezahlukeneyo ze-vialsgraphy yeChromatography kwi-Fift kunye noVavanyo lweSiselo, ukuphonononga kwindima yabo ebalulekileyo ekuqinisekiseni imigangatho yokhuseleko kunye nenqanaba lomgangatho:I-HLLC yesampulu yokulungiselela ukulungiselela iziphumo ezilungileyo

Ngaphambili:

Ngaba ukhe wabelazi uluhlu lwamaxabiso ee-vials ze-HPLC?

Okulandelayo:

Ukubaluleka kwe-vills eqinisekisiweyo ye-HPPL yohlalutyo lweSampulu oluchanekileyo