Ihluke njani i-HPPC kwikholamu ye-GC?

I-HPLC (i-chromatography ye-HPLC) kunye ne-GC (i-chromatography) ziindlela ezinamandla zokuhlalutya ezisetyenziselwa ukwahlula, zichonge izinto ezahlukeneyo zeesampulu ezahlukeneyo. Nangona kunjalo, bahluke kakhulu malunga nokusebenza, izixhobo, kunye nokusetyenziswa. Eli nqaku licacisa umahluko ophambili phakathi kwe-HLLC ne-GLCMNS, igxile kuyilo, ukusebenza, kunye nokufaneleka kweentlobo ezahlukeneyo zohlalutyo.

Uyilo lwekholamu

Iikholamu ze-HPPC

Iikholamu ze-HPLC zihlala zifutshane kwaye zibanzi kune-GC ye-GC. Ziqhele ukuya kuthi ga kwi-30 cm ubude kwaye inee-diameter zangaphakathi ukusuka kwi-2.1 mm ukuya kwi-8 mm. Ukupakisha ngaphakathi kweekholamu ze-HPLC kubandakanya amasuntswana amancinci (okwesiqhelo ngaphantsi kwe-5 microns ububanzi) obunika indawo enkulu yokwaluphala. Iipropathi zokupakisha zale khola zibavumela ukuba zibe neemephu ezahlukileyo ezenziweyo ngokusekelwe kwiipropathi zekhemikhali.

Iimpawu eziphambili:

Ubude: ukuya kuthi ga kwi-30 cm

Ububanzi: Ngokwesiqhelo phakathi kwe-2,1 mm kunye ne-8 mm

Izinto zokupakisha: amasuntsu amancinci (i.G. Silica) ngolungiso olwahlukeneyo lomphezulu ofanelekileyo kwiindlela ezahlukeneyo zokwahlulahlula (i.G. ISigaba esibuyiselweyo, isigaba esiqhelekileyo).

Iikholamu ze-GC

GC columns, by contrast, are longer and narrower, typically up to 100 m in length and have an internal diameter ranging from 0.1 mm to 1 mm. Banokwahlulwa zibe ziindidi ezimbini eziphambili: Iikholamu ezigcwele kunye neekholamu ze-capillary. Iikholamu ezipakishwe zinenqanaba lesikhululo esiliqili okanye i-zungud kwinkxaso eqinileyo, ngelixa iikholamu ze-capillary zinefilimu enenqanaba elinamanqanaba aphantsi kodonga lwangaphakathi.

Iimpawu eziphambili:

Ubude: ukuya kuthi ga kwi-100 m

Ububanzi: Ngokwesiqhelo phakathi kwe-0.1 mm kunye ne-1 mm

Iindidi: Iikholamu ezipakiweyo (iSigaba esiSwentiweyo okanye esiShishini) kunye neekholamu zeCapillary (i-Tublar Gubler).

Isigaba sefowuni

Intsebenzo ephezulu ye-chromatography

Kwi-HPPC, iSigaba seSoftway sisimo se-solvent yolwelo okanye umxube we-polar okanye izicoci ezingezizo ezingoobhontsi. Izinto eziqhelekileyo eziqhelekileyo ziquka amanzi, i-methanol, i-acetostit, kunye nee-buffers ezahlukeneyo. Ukukhetha inqanaba lesigaba sefowuni kubalulekile kuba kuchaphazela ukusebenzisana phakathi kohlalutyo kunye nenqanaba lesikhululo ngaphakathi kwikholamu.

I-chromatography yegesi

I-GC isebenzisa isigaba se-GASOOOOOS, ixhaphake kakhulu irhasi ye-intert enje nge-helium okanye initrogen. Isampulu kufuneka iguqulwe ngokwaneleyo ukuba ikhuphe xa kwazise kwikholamu. Le mfuneko ithetha ukuba i-GC ilungele ukuhlalutya iziqu eziguqukayo, ngelixa i-HPPC inokuphatha uluhlu olubanzi lwezinto, kubandakanya imichiza engaqhelekanga.

Indlela yokwahlula

Intsebenzo ephezulu ye-chromatography

I-HPPC yahlulahlula amakhonkco asekwe kubumbano kwiSigaba seSigaba seSitimela kwiSigaba seSoftware. Iindlela ezahlukeneyo zeChromatography zinokuqeshwa:

Inqanaba lesigaba se-chromatography se-chromatography

Isigaba sesigaba sesiqhelo se-chromatography: isikhululo samaShishini esiPhakamileyo esineSigaba se-Noporlar.

I-ion yokutshintshiselana i-chromatography: Ukwahlula iintlobo kwabo kwiSigaba sokuShicilela amatyala.

Ubungakanani bokukhutshelwa kweChromatography: Ukwahlula iimolekyuli ngokubhekisele kubukhulu.

I-chromatography yegesi

Kwi-chromatography yegesi, ukwahlukaniswa kufezekiswa ngumahluko ekuzingcoleni nasekubhinqiseni kwabahlalutyi. Imigqaliselo ekhutshiwe ngokulula Ukudibana phakathi kohlalutyo kunye nenqanaba lesikhululo linokuchaphazela ixesha lokugcina.

Imvakalelo kunye nesisombululo

Imvakalelo ye-HPLC

I-HPPC ngokubanzi inezinto ezinobuntu obungenamdibaniso ngenxa yokuba iyakwazi ukuhlalutya ukugxininisa ezantsi kweesampulu ngaphandle kokuphuma kwamanzi. Ukusebenzisa ubungakanani be-particle ye-pares kwiikholamu ze-HPLC kubonelela kwindawo enkulu yomphezulu wonxibelelwano, oluphucula isisombululo.

Imvakalelo ye-GC

Kuba i-chromatography yegesi iyakwazi ukugxila ekuhlalutyeni ngokuvela, iyakwazi ukufezekisa ubuntununtunu obuphezulu kwiimvakalelo eziguquguqukayo. I-Capillary Iikholamu ngokubanzi zinesisombululo esingcono kuneekholamu ezipakiweyo ngenxa yobude babo obude kunye nobubanzi obuncinci.

Izicelo ze-HPLC kunye ne-GC

Izicelo ze-HLLC

I-HPPC isetyenziswa kwiintlobo ezahlukeneyo zamasimi ngenxa yokuqalisa i

Uhlalutyo lwamachiza: Isetyenziselwa uvavanyo lokuqulunqwa kweziyobisi kunye nolawulo lomgangatho.

Ukuvavanywa kokusingqongileyo: hlalutya ungcoliseko lwamanzi kunye neesampulu zomhlaba.

UVAVANYO LOKWENKXOBO YOKWENZAYO: Fumana ungcoliseko kwaye uqinisekise umgangatho wokutya.

I-Biotechnology: hlambulula iiproteni kunye ne-nucleic acids.

Izicelo ze-chromatography zegesi

I-GC isetyenziselwa ukuhlalutya iziqu ezisebenzayo eziguqukayo:

Uhlalutyo lwendalo esingqongileyo: Ukulinganisa imigangatho yezinto eziphilayo eziguqukayo kwiingcongconi zomoya kunye namanzi.

Inzululwazi ye-Forensic: Izinto zokuhlalutya izixhobo kwimimandla yolwaphulo-mthetho.

Umzi-mveliso we-petrochemical: uphawu lwe-hydrocarbs kwi-fuels.

I-flavour kunye nohlalutyo lwe-aroma: Ukuchonga izinto eziguquguqukayo ekutyeni.

Kwisishwankathelo, i-HPPC kunye ne-GC ziindlela ezahlukeneyo ze-chromatographic ezilungele iindlela zohlalutyo ezisekwe kuyilo lwabo lwekholamu, iSigaba seSoftware, isicelo, kunye nezakhono zokusombulula. I-HPPC ilungele izixhobo ezingezizo ezingezizo eziguqukayo okanye ezifuna iSigaba seSoftware, ngelixa i-GC igqwesa ekuhlalutyeni izinto eziguqukayo Sebenzisa isigaba se-Gastaous. Ukuqonda lo mahluko kuvumela abaphandi ukukhetha indlela efanelekileyo kwiimfuno zabo zohlalutyo, ukuqinisekisa iziphumo ezichanekileyo kwimimandla eyahlukeneyo.

Ngaba uyawazi umahluko phakathi kwee-vials ze-HPLC kunye ne-GC Vials? Jonga eli nqaku: Nguwuphi umahluko phakathi kwee-vials ze-HPLC kunye ne-GC Vials?