Xa kufikwa kuqinisekisa ukuthembeka kunye nokuthembeka kweziphumo zohlalutyo kwizimo zelebhu, ukhetho lwesIzixhobo zokucoca i-yenringibalulekile. Izixhobo ezahlukileyo ezibonisa ukusebenza kakuhle kokugcinwa kwethagethi, ezinokwenza ifuthe kumgangatho weesampuli ezilungiselelwe uhlalutyo, ngakumbi kwiindlela ezifana ne-chromatography ye-chromatography ye-chromatography ye-chromatography (i-HPPC).

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IIMPITI ZOKUGQIBELA EFANELEKILEYO UKUGCINWA KWEEMPAHLA

I-1. Ubungakanani be-pore

Ubungakanani be-poree yefilitha yenye yenye yezona zinto zibalulekileyo ezichaphazela ukusebenza kakuhle. Isakhiwo esiqhelekileyo se-pore siquka:

I-0.22 μm sinyikihla IifilithaEzi zihluzi zilungele ukusetyenziswa kwezicelo ezifuna ukususwa kweentsholongwane kunye namasuntswana amancinci. Ziqhelekile kwiinkqubo zokuchitheka kunye nokucoca imithombo yee-sel. Ngenxa yobukhulu becala labo oluhle, babonelela ngenqanaba eliphezulu lokugcina izinto, bahlala bedlula kwi-98% yamasuntswana amakhulu ku-0,22 μm.

I-0.45 μm sinyikihla IifilithaEzi zihluma zilungele ukususwa ngokubanzi kwe-General, kubenza babe kukhetho oluthandwayo lwe-HLLC yesampulu ye-HLLC. Ngelixa bavumela iirhafu ezikhawulezayo ezikhawulezayo ezithelekiswa ne-0.22 μm ifilitha, zinokugcina amasuntswana ambalwa, ahlala ejikeleze i-90-95 ~ i-0.45 μm.

Ukukhetha ubungakanani be-pore ofanelekileyo kufuna ukulinganisa ukulungelelanisa ukuqina kunye nenqanaba lokuhamba. I-pore encinci ye-pore ibonelela ngokugcinwa okuphezulu kodwa inokuthoba inkqubo yokuphosa.

Ufuna ukwazi ulwazi olupheleleyo malunga nesihlulo sesiringe, nceda ujonge eli nqaku:Isikhokelo esibanzi kwiFilimeter Syringe: Iimpawu, ukhetho, ixabiso, kunye nokusetyenziswa

2. Izinto zokucoca

Izinto zefilitha yesirinji idlala indima ebalulekileyo ekuqinisekiseni ukusebenza kakuhle kwawo. Nazi ezinye izinto eziqhelekileyo ezisetyenziswa kwiifilithe zesirinji:

I-Polytetrafluoroothylene (PTFE): Yaziwa ngokuba kukungavisisani kwemichizaPtfe sinyikihla Iifilithazilungele izinyibilikisi ezinobunkunkqele kunye nezicelo zobushushu obuphezulu. Ngokwesiqhelo babonisa amanqanaba aphezulu okugcina amachaphaza, ahlala ejikeleze i-98-100% yamasuntswana amakhulu kune-0.45 μm, enza balungele izicelo ze-HLLC.

Nylon: Nylon sinyikihla Iifilithazitshintshiwe kwaye zinokusetyenziselwa zombini izinyibilikisi ezinamanzi nezixhobo eziphilayo. Babonisa ukugcinwa kwe-surticle elungileyo kodwa kunokubanakho ukusebenza kakuhle xa kuthelekiswa neefilitha ze-pmpfe, ngakumbi kwiindawo ezinomsindo.

Polyeherulfone (Pes): I-pes sinyikihla Iifilithabayaziwa ngokuba ziipropathi zabo zeproteni zeproteyini eziphantsi, zibenza bazilungele ukufihlakala kwesampulu. Babonelela ngamanqanaba okugcina amaxhoba, ngokufuthi malunga ne-95-98% ye-0.22 μmmms.

I-Isulonese yeSellXOSE Nangona kunjalo, izifundo zibonise ukuba iifilitha ze-RC zinokuba namazinga asezantsi okugcina, ngamanye amaxesha eziphantsi njenge-4.45 μm yefilitha, ichaza inxenye ebalulekileyo yexabiso elinokudlula.

I-Telupelose exubeneyo (i-MCE):I-MCE sinyikihla Iifilitha zinamachaphaza kakhulu kwaye zilungele izisombululo zezenzi. Ziqhele ukusetyenziswa kuhlalutyo lwe-microbiological ngenxa yokugcina kwabo i-microorganisms, kodwa ukusebenza kwabo kunokwahluka ngokusekwe kwisicelo esithile.

3. Ububanzi be-filitha kunye noyilo

Ububanzi befilitha yesirinji bunokuphembelela ukugcinwa kwalo. Iifilitha zedayimitha ezikhulu zinokuba negalelo eliphezulu, zivumela iirhafu ezinqabileyo zokuchitha, kodwa zinokuba nesayizi eyahlukileyo yobungakanani be-pore, echaphazela ukusebenza kakuhle. Ukongeza, uyilo lwecebo lokucoca ulwelo, njengobukho beefiltha zangaphambi okanye unyango oluthile, lunokuphucula ukugcinwa.

Ukuthelekisa ukusebenza ngemvume kwezihluzi ezahlukeneyo zesirinji

Ngokusekwe kwizinto ezixoxwe, nantsi isishwankathelo sokugcina inyathelo lokufumana igalelo kwizinto ezahlukeneyo zokucoca izixhobo:

Izixhobo zokucoca	Ubungakanani be-pore	Ukugcinwa ngokuqhelekileyo	Ezona zicelo zilungileyo
Ptfe	I-0.45 μm	98-100%	Izinyibilikisi ezinobunkunkqele, i-HPPC
Nylon	I-0.45 μm	90-95%	Ukusetyenziswa ngokuBanzi, i-Aught
I-pes	I-0.22 μm	95-98%	Iisampulu zebhayiloji, iprotein ephantsi
I-selsulose ye-sell	I-0.45 μm	48-50%	Izisombululo ezizenziweyo, uhlalutyo lwe-microbiological
I-Seles ye-Seleelose	I-0.45 μm	Iyahluka	Uhlalutyo lwe-microbiological

Ukuqukumbela

Ukukhetha izinto zokucoca i-Simeri ze-Syringes kubalulekile ekuqinisekiseni ukusebenza kakuhle kwezicelo zelebhu, ngakumbi uhlalutyo lwe-HPPC. Iifilitha ze-PPTO ngokubanzi zibonelela ngeyona ndawo iphakamileyo, ibenze balungele izinyibilikisi kunye nezicelo ezibalulekileyo. Ngokwahlukileyo, iifilti ze-selgelose ezihlaziyiweyo zisenokunganikezeli ekugcinweni ngokwaneleyo kwizicelo ezithile, ukungathobeki ngokunyaniseka.

Xa ukhetha Isihluzi sesirinji, cinga ngeemfuno ezithile zohlalutyo lwakho, kubandakanya uhlobo lwesampulu, kunye nokuhambelana nokusebenza kakuhle. Ngokuqonda umahluko ekugcinweni kwe-subricle kwi-filticle phakathi kwezixhobo ezahlukeneyo zecebo, unokwenza isigqibo esinolwazi ophucula umgangatho kunye nokuthembeka kweziphumo zohlalutyo. Umoya oqingqiweyo awukhusela kuphela izixhobo zakho, kodwa nayo inegalelo kwimpumelelo yelebhu yakho.

Ngaba uyazi ukuba isihluzi sesirinji singasetyenziswa? Nceda ujonge eli nqaku: Iifilitha zesirinji, uya kugqiba?

Ngaphambili:

I-PHE-ST-SLIT CAP VS. I-cap ye-cap: yeyiphi engcono kwilebhu yakho?

Okulandelayo:

I-HPPC vs. Capillary Electrophoreris: Yeyiphi oza kuyisebenzisa?