Yintoni ukubaluleka kwe-1.5ml ye-GC VILS kuhlalutyo lwesampulu?

Ukusetyenziswa kwe-1.5 ML yegesi yegesi yegesi (i-GC) ibalulekile kwicandelo le-chemistry yohlalutyo, ngakumbi kuhlalutyo lwezixhobo ze-ortiber eziguquguqukayo (VOCs). Ezi vils zibalulekile kwizicelo ezahlukeneyo, kubandakanya ukuhlolwa kwendalo esingqongileyo, uvavanyo lokhuseleko lokutya, kunye nohlalutyo lwamayeza. Le blog iya kuthatha i-dive enzulu yokubaluleka kweI-1.5 Ml GC VialsKwisampula uhlalutyo, egxile kwizibonelelo zabo, izicelo zabo, kunye neendlela ezilungileyo.

Ngolwazi oluthe kratya malunga ne-vials ye-autosampler yegesi chromatography, ibhekisa kweli nqaku: I-2 ml automampler vials ye-chromatography yegesi

Ukubaluleka kwe-1.5 ml GC vials

I-1. Umthamo ofanelekileyo wohlalutyo lweentloko

Esona sizathu siphambili sokusebenzisa i-1.5 ml vials kukuba zezona zibalaseleyo zesampulu ye-PREAP. Kuhlalutyo lweentloko-ntloko, indalo etywiniweyo ivumela i-VOCs ukuba ifikelele ngokulinganayo phakathi kolwelo okanye isampulu eqinileyo kunye nenqanaba legesi ngaphezulu kwayo. I-1.5 ml ivolutha ibhalansi phakathi kokubonelela ngenxalenye eyahlukileyo yesampulu yegesi kwaye inciphise amandla elahleko yesampulu ngenxa ye-adsorption kumphezulu we-vial.

I-Equilibribribribribribribribribribribribribrics: ivolumu encinci, ulungelelwaniso olukhawulezayo phakathi kwezigaba lunokufundwa, olubaluleke kakhulu ekuhlaziyeni ukuhlalutya uluntu. Inqanaba lesigaba segesi yinto ehlalutyayo i-GC ekugqibeleni, ngoko kubalulekile ukuba eli nqanaba limele isampulu.

I-2. Ukuncitshiswa kwe-adsorction

I-adsorvotion inokubangela iindidi eziguqukayo ukuze zilahlekelwe kwiindonga ze-vial, ezinokuchaphazela kakubi ukuchaneka kweziphumo zohlalutyo. Umgangatho ophezulu we-1.5 ml owenziwe kwiglasi ye-hydrolyzdymed ye-hydrolyzed zenzelwe ukunciphisa ezi ziphumo.

Ipropathi ye-adsorption ye-adsorption: Uninzi oluqinisekisiweyoI-1.5 Ml Vialsbaphathwa ukunciphisa i-adsen, ngakumbi amaqumishini esisiseko. Olu nyango luphucula ubuchule bokuchaneka kwaye luqinisekisa ukuba uhlalutyo luhlala lungasebenzi ngexesha lohlalutyo.

I-3. Ukuhambelana neendlela ezahlukeneyo zohlalutyo

Ukusebenza kwezi-1.5 ml vials akupheleli kwi-GC; Zikwalungele ukufumana i-chromatography yegesi yegesi-Mass Spectrometry (GC-MS) Izicelo.

Iguquguqukayo: Yenzelwe ukulungiselela iindlela ezahlukeneyo zohlalutyo, zivumela iilebhu kwiinkqubo zokulinganisa ngaphandle kokuzilungiselela iintlobo ezininzi zebhotile yokuqalisa. Oku kubenza lula ulawulo lweempahla kunye nokunciphisa iindleko.

Izicelo ze-1.5 ml GC vials

1. Ukuhlolwa kwendalo

Ukufumanisa ukulandela umkhondo emoyeni okanye kwiisampulu zamanzi kubaluleke kakhulu kwizifundo zokusingqongileyo.

Uhlalutyo lweVOC: 1.5 I-Vials ilungele ukufumana iipowus zokusingqongileyo ngaphandle kwelahleko enkulu ngenxa ye-adsorction okanye ukuqinisekisa ukuba iziphumo zibonisa ngokuchanekileyo ukwakhiwa kwesampulu yesampulu yesampulu.

2. Uvavanyo lokhuseleko lokutya

Ukukhuseleka kokutya kubalulekile ekuqinisekiseni impilo yoluntu, kwaye imijikelezo ye-witleile ihlala ibonisa i-chilage yokutya okanye ungcoliseko.

I-flavour kunye ne-aurows: Ukuhlalutya i-flavouse kunye ne-aurounds ekutyeniI-2 ml vialsIvumela ulawulo olusemgangathweni oluchanekileyo kunye noKhuseleko.

I-3. Uhlalutyo lwamayeza

Ukuhlalutya okuguqukayo okanye izithako ezisebenzayo kubalulekile kuPhuhliso lweziyobisi kunye nolawulo olusemgangathweni.

Uvavanyo lokuzinza: Ivolumu encinci iluncedo kakhulu kwizifundo, njengenani eliqingqiweyo lemilinganiselo yeziyobisi iyafumaneka kuvavanyo.

Ngaba uyawazi umahluko phakathi kwee-vials ze-HPLC kunye ne-GC Vials? Jonga eli nqaku:Nguwuphi umahluko phakathi kwee-vials ze-HPLC kunye ne-GC Vials?

Ezona ndlela zibalaseleyo zokusebenzisa i-1.5 ml vials

1. Iindlela ezifanelekileyo zokutywinwa

Ukuqinisekisa ukuba i-vials itywinwe ngokufanelekileyo kubalulekile ukuthintela ukuvuza kunye nongcoliseko:

Sebenzisa iSepta ekumgangatho ophezulu: KhethaSeptayenziwe ngezinto ezinje nge-ptfe okanye i-silicone, ezibonelela ngeepropathi ezigqwesileyo kwaye azizukukhupha kwisampulu.

2. Ulawulo lwevolumu yesampula

Kubalulekile ukulawula isixa sesampulu ye-fluid isihlobo sesayizi ye-Vial:

Inqanaba elipheleleyo lokugcwalisa: Ngokufanelekileyo, ulwelo kufuneka lube malunga ne-10 ukuya kwi-50% yevolumu ye-vial ukubonelela ngesampulu eyaneleyo yesampulu yegesi ngelixa unqande ukunxibelelana kakhulu neendonga ezinqabileyo.

3. Ulawulo lobushushu ngexesha lohlalutyo

Iqondo lokushisa lidlala indima ebalulekileyo ekufumaneni iziphumo ezichanekileyo:

Ukufudumeza okuqhubekayo: Gcina ubushushu obungapheliyo ngexesha lenkqubo yokulingana ukuqinisekisa ukuba i-VOCS ifikelela ngempumelelo. Umahluko ngexesha lohlalutyo lunokubangela utshintsho olukhulu kwiindawo eziphakamileyo.

4. Ukulinganisa rhoqo kunye nokulungiswa

I-Procine Colibration kunye nokugcinwa kweesampulu ze-vial kunye nezixhobo zokuhlalutya kubalulekile:

Jonga ingqibelelo ye-phem: vula i-vills ye-vill okanye iziphene ezinokuthi zichaphazele iziphumo.

Ukulinganisa isixhobo: Qinisekisa ukuba inkqubo ye-GC ilungelelaniswe ngokomlo wokugcina ukuchaneka kwexesha elide.

Ukuqukumbela

Ukubaluleka kweI-1.5 Ml GC VialsKuhlalutyo lwesampula alunakuba ngaphezulu. Ubungakanani bayo obufanelekileyo buqokelele isampulu ye-ERPECE ye-ERPE ye-REATACE ngelixa kuncitshiswa iziphumo zo-AdsorK, zenza ilungele uluhlu olubanzi lwezicelo ezivela kwiliso kwindalo esingqongileyo kuvavanyo lwe-PHEMOCOCAC. Ngokuthobela ezona ndlela zibalaseleyo zobuchule, ulawulo lwevolumu, uLawulo lobushushu, kunye nokuLondolozwa rhoqo, iiLabhoratri zinokuqinisekisa iziphumo ezisemgangathweni ezisemgangathweni ezihlangabezana nemigangatho yohlalutyo.

WImbovane yokwazi ngakumbi malunga nomahluko phakathi kwe-LC-Nks kunye ne-GC-Nks, nceda ujonge eli nqaku: Nguwuphi umahluko phakathi kwe-LC-Nks kunye ne-GC-Nks?