E-China, inani elikhulu leemveliso zezolimo (ezinye iimveliso zeekhemikhali, i-organic acids, njl.) kufuneka zichongwe nge-chromatography yolwelo kunye ne-chromatography yegesi minyaka yonke. Ngenxa yenani elikhulu leisampulu, inani elikhulu leiibhotile zesampulu yechromatographic idinga ukucocwa ngexesha lenkqubo yokubhaqa, engachithi nje ixesha kwaye inciphise ukusebenza kakuhle, kodwa ngamanye amaxesha ibangela ukutenxa kwiziphumo zovavanyo ngenxa yokunqongophala kococeko lweebhotile zesampulu zechromatographic emva kokucoca.

Ukucocwa kweebhotile zesampulu yechromatographic kubaluleke kakhulu. Ngokwendlela yokuhlamba isixhobo seglasi, khetha indlela yokucoca ngokwenqanaba longcoliseko, akukho ndlela emiselweyo. Indlela isishwankathelo:

Isisombululo 1:

1. Galela kwaye womise ulwelo kwiIsampulu yesampulu yekhromatographic vial.

2. Galela owomileyo emva koku njengoko utywala bungena kwi-vial ye-1.5mL ngokulula kwaye i-intersoluble kunye nezinyibiliki ezininzi ze-organic.

3. Thela emanzini kwaye uhlambe nge-ultrasonic kabini.

4. Thela i-liquid kwibhotile kwaye ubhake kwi-110 ° c nge-1 ~ 2 iiyure. Musa ukubhaka kwiqondo lokushisa eliphezulu.
5. Pholisa kwaye ulondoloze

Isicwangciso 2:

1. Hlanza amanzi etephu amaxesha amaninzi

2. Zifake kwibhekile ezaliswe ngamanzi acocekileyo kunye ne-ultrasound imizuzu eyi-15

3. Guqula amanzi kunye ne-ultrasound enye imizuzu eyi-15

4. Gxumeka kwibhekile ene-ethanol okanye ngaphandle kwayo

5. Khupha i-zui kunye nomoya owomileyo

Ngaba uyafuna ukwazi ukuba ungazenza njani iivial ezahlukeneyo zeHPLC?, Jonga eli nqaku:I-Crimp Vial vs. Snap Vial vs. Screw Cap Vial, Indlela yokukhetha?

Isisombululo 3:

1. Gcoba nge-methanol (i-chromatographic pure) kwaye ucoce nge-ultrasonic imizuzu engama-20, uze uthele i-methanol eyomileyo.

2. Gcwalisa ibhotile yesampuli yechromatographic ngamanzi, uhlambulule nge-ultrasonic imizuzu engama-20, kwaye ukhuphe amanzi.

3. Emva kokumisaiibhotile ze-hplc

Isisombululo 4:

1. Ngokubanzi kukusetyenziswa kokuqala kwamanzi acocekileyo omiswe kwaye emva koko usebenzise i-sulfate dichromate potassium lotion immersion.

2. Indlela yokuhlamba ibhotile yesampuli yechromatographic iyafana naleyo yesigaba solwelo. Okokuqala, utywala lwezonyango lusetyenziselwa ukucwina iiyure ezingaphezu kwe-4, kwaye emva koko i-ultrasonic isiqingatha seyure

Isisombululo 5:

1. Ukuba iindleko zanele, sebenzisa i-zui entsha rhoqo

2. ukuba ufuna ukuphinda usebenzise kwakhona, indlela yokucoca nayo ibaluleke kakhulu, okokuqala ngesisombululo esinamandla sokucoca i-oxidation (i-potassium dichromate) soak iiyure ezingama-24, emva koko ngamanzi ahlanjululweyo phantsi kwemeko ye-ultrasonic kathathu, emva kwe-zui ngokucoca i-methanol, ukomisa kungasetyenziswa.

3. I-MATS yebhotile mayitshintshwe kufakwe ezintsha, ngakumbi xa kuhlalutywa iintsalela ze-pesticide, kungenjalo iziphumo zobungakanani ziya kuchaphazeleka.

Wan ukwazi ulwazi olupheleleyo malunga nendlela yokucoca iibhotile zesampulu yechromatography, nceda ujonge eli nqaku:Esebenzayo ! Iindlela ezi-5 zokucoca iibhotile zesampulu zechromatography

Ezi zingentla zihlanu iindlela zokucoca iibhotile zesampuli zechromatographic, sinethemba lokuba sinokuqwalasela ukucocwa kweebhotile ze-hplc.

Ngaphambili:

Ichromatograph isebenzisa iivials ezingenanto kunye nesihluzo sesirinji seHPLC

Okulandelayo:

Indlela yokupakisha i-20ml ye-chromatograph vial

IITAGS: ii-hplc-autosampler-vials 2ml-gc-vial 2ml-isampulu-iibhotile ibhodlela-1.5ml

Ukubuza