Ungayikhetha njani phakathi kwe-HLLC kunye ne-GC Vials? Ukuthelekiswa nobunzulu bezinto kunye nokuhambelana

Intshayelelo

Ngohlalutyo lweChromatographic, ukukhetha isampulu ye-vial ichaphazela ngokuthe ngqo ukuchaneka kunye nokuzala kweziphumo zakho. Eli nqaku libonelela ngokwahlukana okuthile phakathi kwe-HLLC kunye ne-GC I-vila, igxile kwiindlela zesicelo, iimpawu zezinto ezibonakalayo, kunye nokwenza ukuba ukhethe ngezesayensi kunye nokuphucula ukusebenza kwelebhu.

I. Umahluko ophambili phakathi kwe-HLLC kunye ne-GC Vials

1. Imeko yesicelo

I-HPLC Vials: Ikakhulu isetyenziswa kwi-chromatography ye-chromatography (HPLC) kunye nohlalutyo lwe-LC-MS, ilungele iisampulu ezingathandekiyo, ezingafanelekanga, okanye ezibuthathaka.

I-GC Vials: Isetyenziswe kwi-chromatography yegesi (GC) kunye ne-GC-Nks, ifuna ukumelana nobushushu (ngesiqhelo> 300 ° C) kunye nokuhambelana nezinyibilikisi eziguqukayo.

I-2. Izinto ezibonakalayo kunye nokuyila amanqaku

Iindidi zentamo zeVenkile:

I-HPPC:Sebenzisa i-screw tops okanye i-snap tops, efanelekileyo yokufikelela okuphindiweyo kunye neesampulu ezizenzekelayo.

I-GC:Ngokwesiqhelo i-crimpp i-vials ephezulu ye-aluminium Caps kunye ne-ptfe \ / isiliki sepga ye-Septa yokutywina kobushushu obuphezulu.

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Ukukhetha izinto:

I-HPPC:Ngokwesiqhelo senziwe ngeglasi ecocekileyo ye-borossilicate, kunye neenguqulelo ze-amber zeesampulu ezibuthathaka.
I-GC:Ifuna ukuxhathisa ubushushu obuphezulu-iglasi ye-borosililicate ixhaphakile, ngeplastiki ye-PFA esetyenziswa kwizicelo ezikhethekileyo.

II. Ukuqwalaselwa okuphambili kwezinto ze-chicromatography

I-1. Izixhobo zeglasi

Iglasi ye-borosililicate: I-Intert kakhulu kunye nokungaxhathisi ubushushu; Ilungele zombini i-HLPC kunye ne-GC.
Iglasi ye-Amber: Inika ukhuseleko lokukhanya kweesampulu ezibuthathaka.

2. Izinto zeplastiki

I-Polypropylene (PP): Ixabiso elisebenzayo kwaye lilungele ukusetyenziswa kweqondo lobushushu; Isisombululo esilinganiselweyo kunye nokuxhathisa ubushushu.
I-PerfluoAlkoxy (PFA): Ukuxhathisa okuphezulu kunye nokungangqinelani kwemichiza; Ilungele ukulandelela kunye nobushushu obungaphezulu kweqondo le-GC.

Funa ukuphononongaYintoni i-GC entloko? Cofa apha kwinkangeleko yobunzulu

I-3. I-cap kunye ne-Septa

Ptfe \ / isilicone Septa: Thintela ukungena kwi-HLLC; Gcina ubume phantsi kobushushu kwi-GC.
Iindlela zokutywina: I-GC CRIMP CAPS ifuna izixhobo ezizodwa, ngelixa i-HPLC SCAP kulula ukuyiphatha.

III. Imiba yokuhambelana nendlela yokuphepha

1. Isixhobo sokuziqhelanisa

I-autosampler: Qinisekisa ukuphakama kwe-vial kunye ne-diameter ye-diameter yetreyi (i.G., 2 ML Dials).
I-Septa Punction Ukunganyangeki: Inaliti rhoqo kwi-HPPC, khetha isethostity ye-ellaistity (E.G., PTFE \ //).

Ukujongana ne-vial caps kunye ne-septa, kufuneka uyazi Chukuma apha

I-2. Ukulawulwa komngcipheko

Ukuphepha iziphazamiso: Kwiqondo eliphezulu le-GC, khetha iglasi ye-inter okanye i-PFA Vials.
Ukuthintela ungcoliseko olunqamlezileyo: Sebenzisa i-vials ecocekileyo ye-ECONS (E.G., iklasi ye-USP a).

Iv. Ukuthenga isikhokelo: Ukukhetha i-vial ye-HPLC kunye ne-GC

Chaza iimfuno zovavanyo: Qwalasela uluhlu lobushushu, ukuguquguquka kwesampulu \ / imvakalelo yokukhanya, kunye nenaliti.
I-SAMFAY YOKUGQIBELA: Qinisekisa uhlobo oluchanekileyo lwentamo, ivolumu, kunye ne-autosamumpler.

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Jonga kwimigangatho yeshishini: Sebenzisa i-vials nge-USP okanye isiqinisekiso se-ISO sokungqinelana okusemgangathweni.
UVAVANYO LWEZIBONELELO: Kwiimvavanyo eziqhubeka rhoqo, khetha i-vials yeglasi efikelelekayo; Ukuhlaziywa kohlalutyo, khetha izixhobo ze-Inert.

V. I-FAQ-IMIBUZO EYAPHENDULA

Q: Ngaba i-HPLC isetyenziselwa uhlalutyo lwe-GC?
A: Ukuba yenziwe ngeglasi ye-borossililicate kunye nokuxhathisa ubushushu (i.G., ukuya kuthi ga kwi-400 ° C), banokusetyenziswa okwethutyana. Nangona kunjalo, ukusetyenziswa ixesha elide phantsi kobushushu obuphakamileyo akucetyiswa.

Q: Ungaluphepha njani ungcoliseko lweVal?
A: Khetha i-vials esuswe kwangaphambili okanye icoceke i-ultracolly usebenzisa i-chromatographic yenqanaba le-chromatographic.

Q: Ngaba i-vials ephezulu ye-cruals eyimfuneko kwi-GC?
A: I-Crimpp Vials ibonelela ngokuthe kutyhila ngcono kwiqondo lobushushu eliphezulu, nangona izixhobo ezithile zixhasa i-screw caps ngeeadapter.

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Ukuqukumbela

Ukukhetha i-HPLC yasekunene okanye i-GC ye-GC ifuna ukuthathelwa ingqalelo kweemeko zovavanyo, isixhobo sokuhambelana, kunye neempawu zesampulu. Ngokwenza izinto ezifanelekileyo ze-vial kunye neenkcazo, unokunyusa kakhulu ukuthembeka kwedatha kunye nokunciphisa iindleko zovavanyo ngokubanzi.