Intsebenzo ephezulu ye-chromatography .

Ufuna ukwazi iimpendulo ezingama-50 malunga nee-vials vials, nceda ujonge eli nqaku:I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

Indlela yokwahlula

I-HPPC isebenzisa i-hydrophobic okanye uxinzelelo lwe-hydrophilic yokwahlukana. Ingahlulahlula uluhlu olubanzi lweengcongconi zezinto eziphilayo, zombini ezi-onrolar kunye ne-polar, usebenzisa iintlobo ezahlukeneyo zezigaba zesikhululo kunye nezinyibilikisi zezinto eziphilayo. Ukwahlula-hlula kuxhomekeke kubudlelwane bendawo yohlalutyi kwiSigaba seSikhululo, esinokwenziwa ngobuqhetseba ngokokuqikiswa kwe-graent ukwenza isisombululo.

I-IC, kwelinye icala, ikakhulu isebenzisa iindlela zotshintshiselwano lwe-ion yokwahlulahlula i-Ionic kunye nePolar. Inqanaba lesikhululo liqhele ukudityaniswa kwe-ion yokutshintshiselana, ivumela ukwahlulwa ngokuphumelelayo kwe-anyanisi kunye neesanti. I-IC isebenzisa i-accuents eqondakalayo, ihlala inamanzi okugqibela ngeetyuwa okanye ii-acids, kwaye uninzi lokwahlula lube sengqiqweni ngaphandle kwesidingo sesifundo seRadient.

Uhlalutyo

I-HPLC iphinde ihambelane nobubanzi obubanzi bezinto eziphilayo, zenza ilungele ukusetyenziswa kwiindawo zamayeza, ukutya, kunye nohlalutyo lwendalo. Nangona kunjalo, inomda xa kufikwa kumba wokwahlulahlula iintlobo zee-IIIC, ezinje nge-anaon kunye nee-cultions, ezihlala zingagcinwanga ngokufanelekileyo kwiikholamu ze-HPLC.

I-ic i-ic i-ic i-ic ekuhlalutyeni iintlobo ze-Ionic kunye ne-polar, iyenza indlela ekhethiweyo yokufumanisa ii-ahoris zomgangatho (njenge-chloride kunye ne-fluoride) kunye nee-fluoride) kunye neesakhiwo. Iluncedo kakhulu kwimihlaba enjengokujonga okungqongileyo, ukhuseleko lokutya, kunye nohlalutyo lwamayeza, apho i-Ionic Compleges ixhaphakile. I-IC inokufumana kwakhona i-Un-Ion-Ion-Ionijel Polands xa kusetyenziswa ikholamu efanelekileyo kunye nesigaba sefowuni.

Ungakanani na ukuba wazi malunga ne-HPPC yohlalutyo ye-HPPLC? Nceda ufunde eli nqaku ukuze ufunde ngakumbi:Nguwuphi umahluko phakathi kokuhlalutya i-HPLC?

Iindlela zokufumanisa

I-HPPC eqhelekileyo isebenzisa izixhobo ze-UV, ezifuna uhlalutyi ukuze ifumane ukukhanya kwe-Uv. Oku kuzisa umceli mngeni yokufumanisa amakhompom, ngakumbi ezo zingakhanyisi, ezinjengee-ions kunye ne-acids.

I-IC ihlala ihlolwa ngokuqinileyo, esebenzayo kwiintlobo ze-Ionic. Indlela inokufezekisa ubuntununtunu obungaphelelayo ngeendlela nje zokucinezelwa kweekhemikhali, ezivumela ukuqhutywa kwemvelaphi, kuvumelekile ekufumaneni amanqanaba alandelelayo amanqanaba ohlalutyo.

Izicelo

I-HPLC isetyenziselwa ngokubanzi uhlalutyo lwezinto ezinobunzima obunzima, kubandakanya amayeza, oongezo lokutya, kunye neesampulu zokusingqongileyo. Amandla ayo okhupha imilinganiselo efanayo kuyenza isixhobo esinamandla kumashishini ahlukeneyo.

I-IC iluncedo ngakumbi kwizicelo ezifuna uhlalutyo lweentlobo zee-IIIC, ezinje ngovavanyo lomgangatho wamanzi, uhlalutyo lokutya, kunye novavanyo lokufumana amayeza. Ihlala isetyenziselwa ukuncedisa i-HPPC, yandisa amandla oluhlalutyo lweelebhu zokubandakanya uluhlu olubanzi lwe-Ionic kunye nohlahlo lwePolar.

Kwisishwankathelo, ukhetho phakathi kwe-HLLC kunye ne-IC luxhomekeke kwiimfuno ezithile zohlalutyo, kubandakanya uhlobo lweentsontelo ezifundwayo kunye nokuziva kunye nokusonda kunye nokusombulula. HlcIlungele uluhlu olubanzi lweendawo ezisebenza zezinto eziphilayo, ngelixa i-IC yindlela yokhetho lohlalutyo lweentlobo ze-Ionic kunye nePolisi.

Wazi njani ukuba amanyathelo okulungiselela i-vialsgraphy ye-vials yohlalutyo? Funda eli nqaku ukuze ufunde ngakumbi: Amanyathelo ama-6 okulungiselela i-vialsgraphy ye-vials yohlalutyo

Ngaphambili:

I-Static vs Propace GC: Ukuqonda umahluko

Okulandelayo:

Yonke into oyifunayo ukwazi malunga neetyhubhu zovavanyo ze-COD

Iimpawu: I-H Plcanalysis I-HPLC-vils I-HPLC-GLARS-VILS I-HPLC-Vials-Ixabiso I-HPLCVIELDSPLEMS I-HPLC-vials-yentengiso Umvelisi we-HPLC-Vials

Umbuzo