I-chromatography, isiseko sohlalutyo lwe-chemistry, isebenza njengesixhobo esixabisekileyo sokwahlula kunye nokuhlaziywa komxube onzima. Ubuchule buxhomekeke ekunxibelelaneni phakathi kwezinto ezinesampulu kunye nenqanaba lesikhululo njengezixhobo zesampulu zihamba ngesigaba sefowuni. Iizinto ezininzi zinegalelo kwimpumelelo yohlalutyo lwe-chromatographic, kodwa enye yezinto eziphambili ekuqhubekiseni ukusebenza kwayo kukufakwai-micro-faka. Ezi zixhobo ezisithethi, kunye nenani labo elincinci, zidlala indima ebalulekileyo ekuphuculeni ukuchaneka kunye nemvakalelo yenkqubo ye-chromatographic.

Ukuqonda i-chromatography

Ngaba unomdla wokufumanisa inkqubo yokukhetha i-micro-faphu ye-vials yakho ye-chromatography?

I-Chromatography isebenza kumgaqo wokukhetha i-adsorve kunye nokusasazwa. As a sample passes through a stationary phase, the interaction between its components and the medium results in a separation based on the inherent chemical properties of the sample. Iiparamitha ezibalulekileyo ezinjengeekholamu, isisombululo kunye nomthetho-nkqubo ye-peak sinempembelelo ebonakalayo ekusebenzeni kwenkqubo yokwahlulwa kweChromatographic. Imicroinsert inokunceda ukujongana nemiceli mngeni enxulumene nenaliti kunye nentsebenzo, ekugqibeleni ukuphucula ukusebenza kwenkqubo yeChromatographic.

Funa ulwazi malunga nokuqwalaselwa okusebenzayo okukhokelela ekukhetheni iglasi yeglasi kwi-chromatography ?:Kutheni le nto iglasi ye-conical isetyenziswe kwi-chromatography?

6 Indima ikakhulu kwimicrosist

Ukunciphisa ivolumu yesampulu

I-microistsbanamandla xa kusenziwa imiqulu emincinci yesampulu kuluhlu lwe-micronitre. Oku kuyingenelo ikakhulu xa ujongene neesampulu ezilinganiselweyo okanye ezixabisekileyo. Ukusetyenziswa ngokukuko kwenani elincinci kuqinisekisa ukuba iisampulu zibonakala zichanekile kwiiChromatograms, inkunkuma incitshisiwe kwaye izinto ezinqabileyo zinokuhlaziywa.

Thintela isampulu

Ukufaka imiqulu encinci yesampuli kunokukhokelela kwiSampuli yeSampuli, kodwa le nkxalabo iphethwe ngamancinci ukufaka. Oku kufaka isenzo sisebenza njengomqobo wokukhusela, ukukhusela izinto eziguqukayo kwilahleko yangaphambi kwexesha. Oku kukhusela ukuthembeka kwisampulu kunye nokuqinisekisa ukuba iziphumo zohlalutyo zibonisa ukwenziwa kwenyani yezinto zokuqala.

Ukunyusa ukuchaneka kunye nemvakalelo

Ukufaka i-Micro faka Yenza igalelo elibalulekileyo ekuchanisweni kunye nemvakalelo. Uyilo lwabo luvumela ukuba yonke ipasika ingeniswe kwinkqubo yeChromatographic. Le yinto ebalulekileyo ekwinqanaba lokuhlalutywa kwenqanaba. Isiphumo sizicikidi ezibukhali kunye ne-ratiol-to-to-to-ingqokelela yengxolo, ebaluleke kakhulu ekufezekiseni iziphumo ezinokuthenjwa kwaye ziphinde ziphinde ziphinde zihlaziywe.

Ngaba unomdla wokufumana ulwazi olupheleleyo malunga neentlobo ezahlukeneyo zokufakwa okufumanekayo kwi-vialsgraphy ye-chromatography?Funa ukwazi yonke into oyifunayo ukwazi malunga neentlobo ezahlukeneyo zokufakwa kwe-vialsgraphycy

Incitshisiwe

Ingxaki eqhubekayo kwiChromatography, i-Parceover ibhekisa kubukho besampulu yendawo eshiyekileyo kwiinaliti zangaphambili. I-Microinsert ilungisa le ngxaki ngokudibanisa isampulu ngaphakathi kwendawo evaliweyo, ukunciphisa umngcipheko wongcoliseko phakathi kwenaliti. Oku kubaluleke kakhulu ekugcineni ukucoceka kweesampulu ezilandelayo kunye nokufezekisa ii-chromatoms ezichanekileyo.

Ukuhambelana ne-autosamplers

I-microisert yeyilelwe ngokuhambelana nengqondo kwaye idibanisa ngokungagungqiyo ngeenkqubo ze-autosampler. Oku kwenziwe ngokuzenzekelayo akupela ukuqhutywa kwe-chromatographic, kodwa kunciphisa amandla empazamo yomntu onxulumene nenaliti yeNqaba. Ukulungelelaniswa kweMicrolisert kwiinkqubo ezizenzekelayo kunyusa ukusebenza kakuhle kohlalutyo lwegalelo.

Ukuqukumbela

Njengoko i-chromatography iyaqhubeka ivela, indima yei-micro-fakaibaluleka ngakumbi. Ukukwazi kwabo ukujongana nemiceli mngeni enxulumene nevolumu yesampula, ubuchule bokuphuma kunye nenaliti banegalelo ekuchazeni nasekufundeni imvakalelo efunekayo kwiChemistry yanamhlanje. Njengoko inkqubela phambili yetekhnoloji, ukukhula okuqhubekayo kunye nokudityaniswa kwee-microsert kuya kuba semqaqweni wezinto ezintsha, ukutyhala imida yento enokufezekiswa kwicandelo leChromatography.

Ngaba unomdla wokufumanisa inkqubo yokukhetha i-micro-faphu i-vials yakho ye-chromatography yakho?Ungalukhetha njani uhlobo olufanelekileyo lwe-Micro-fin ye-vials yakho ye-chromatography yakho

Ngaphambili:

Ukubaluleka kwe-septum kwi-vials ye-HPLC: Ukuqinisekisa ukuchaneka kohlalutyo

Okulandelayo:

Yonke into ofuna ukuyazi malunga nokulawula imithombo yeendaba

Iimpawu: Faka i-HPLC I-Automampler-i-vial-vial Ixabiso le-Micro-faka Umthengisi we-vial-faka Intengiso ye-Vial-yentengiso I-GC-vial-vial

Umbuzo