Isampulu ye-vial inefuthe elikhulu. Ichaphazela inkqubo kunye neziphumo ze-chromatography. Ukuba isampulu ye-vial icociwe engalunganga, iya kuyingcolisa. Olu nqam lunokubangela: Iincopho zesiporho, ukonakaliswa kwesampulu, ukuphinda ulahlekelwe, kunye neencopho ezibangelwa sisinyibilikisi kunye ne-septum edibene. I-vials ekhokelwa ngokungafanelekanga iya kuchaphazela kakhulu iziphumo zovavanyo. Oku kuya kubangela ukuphambuka kwiziphumo kwaye uqhubeke nomonakalo kwi-autosampler.

Ke, ukucocaI-autosampler vialibaluleke kakhulu. Umvavanyi kufuneka ayifumane indlela yokucoca kaVial.

Ufuna ukwazi ngokupheleleyo malunga ne-vials ye-HPLC, nceda ujonge eli nqaku: I-Encyclopedia ye-vials ye-HPLC

Kwenziwa kanjani

Indlela yokuhlamba yahlukile ngokwenqanaba longcoliseko lwe-vial. Eli nqaku liza kucebisa ngeendlela ezininzi zokucoca i-vial. Baza kukunceda ugqibe uvavanyo lwe-chromatographic.

Indlela yoku-1

1. Thula isisombululo sovavanyo ngaphakathii-vial vial.

2. Intshukumo yonke i-vial i-vial i-95% yotywala, icocekile kabini, kwaye emva koko igalele (i-ofisi ephezulu yotywala ingena kwi-vial kwaye inokuthi iphindene nezinyibilikisi eziphilayo).

3. Galela amanzi acocekileyo kwaye ucoce kabini.

4. Thumela ulwelo lwe-vial kwaye lubhake kwi-110 degrees Celsius iiyure eziyi-1-2 (musa ukubhaka kwiqondo lokushisa eliphezulu, eliza kubangela ukonakala kwi-vial).

5. Hlamba kwaye ugcine.

Indlela 2

1.Umaxesha amaninzi ngamanzi empompo.

2. Intlukwane kwi-beaker emanzini kwaye isebenzise isicoci se-ultrasonic kwimizuzu elishumi elinesihlanu.

3. Cima amanzi kwaye uyinike eminye imizuzu elishumi elinesihlanu yokucoca i-ultrasonic.

I-4. Susa i-vial kwi-vial kwi-anhydural ethanol egcwele.

5. Ekupheleni, yisuse kwaye uyivumele ngokome ngokwemvelo ngomoya.

Indlela 3

1. Khokela eMetanol kangangemizuzu engamashumi amabini, emva koko ususe i-methanol kwaye uyicoca nge-ultrasonography.

2. Emva kongeza amanzi amaninzi kwi-vial vial, i-ultrasonic icocekile kangangemizuzu engama-20, emva koko iqhume amanzi.

3. Vumelai-vialukomisa.

Indlela 4

I-1. Hlambulula kwaye uyilole ngamanzi acocekileyo kuqala, emva koko uthambise kwi-Arhente ye-Oxiping eCourage (i-potassium Disfate ye-Sulfate) yokucoca.

I-2. Ndithabathele i-tyress yonyango ngaphezulu kweeyure ezi-4, emva koko ucoceke i-ultracloocial kwisiqingatha seyure, emva koko phalaza ulutywala iyure, sebenzisa i-ultradonic ecocekileyo, sebenzisa i-ultrasonic ecocekileyo kangangesiqingatha seyure, hlamba ngamanzi kwaye womile ngamanzi.

Indlela 5

I-1. Thoba kwi-arhente enamandla yokucoca i-oxidition iiyure ezingama-24.

2. Ukucoca izihlandlo ezi-3 ngamanzi aphantsi kwe-deoernic phantsi kweemeko ze-ultrasonic, kwaye ekugqibeleni ucoceke kanye nge-methanol, kwaye yomile ukuze isetyenziswe.

Indlela yesi-6

Sebenzisa umatshini wokuhlamba. Umatshini wokuhlamba ibhotile unokuphucula ukusebenza kwee-vials zokucoca kwaye ugcine amandla omzimba kunye nexesha.

Ngaba uyazi ngakumbi ukuba ucoca i-vials yakho ye-HPPC? Jonga eli nqaku: Iindlela ezintlanu zokucoca ii-vials ze-HPLC

Iingxaki onokudibana nazo

Zininzi iingxaki zokucoca ngesandla.

1. Ukucocwa okuphantsi kokucoca, ixesha elide lokuhlamba, ukutya kunye nomzamo. Ukongeza, inani lebhuloshe kunye nexabiso lebhulashi kunye nezicoci zikhulu, kunzima ukuphatha, kunye nokwenza amanzi amanzi.

2. Ukucocwa Kukwenzelwa kwiikona ezifileyo, isiphumo sokucoca kunzima ukuqinisekisa, sichaphazela iziphumo zovavanyo, kunye nokunyuka kweendleko.

3. Kukho iingozi zokhuseleko kwinkqubo yokucoca yabasebenzi be-vial. Ezinye iilabhoratri ziya kusebenzisa oomatshini bokucoca oomatshini bokucoca. Le ndlela yokucoca iphucula ukucocwa kokucoca kunye nokucoceka ukuya kwinqanaba elithile, kodwa ikwanazo izinto ezingalunganga.

Umatshini wokucoca u-Ultrasonic yingxolo kunye nengqwabalala ngexesha lokusebenza, kwaye kulula ukwaphula i-vial, kwaye ixesha lokucoca akukho lula ukuyiqonda. Ke ngoko, sicebisa ukuba unxibe iigloves, amagqabi, i-gogles, i-emplegs kunye nezinye izixhobo zokhuseleko ngenkqubo yenkqubo yokucoca.

Ukuba i-vial indala okanye yonakele, musa ukuyibuyisela. Kuxhomekeka ekubeni uphinda ulingane kangakananiisampulu ye-vialZonke iinyanga ezi-1-3.

I-vials elahliweyo kufuneka ilawulwe ngokungafaniyo, ilawulwa ngokungqinelana neMigaqo yeLabhoratri yoLawulo lweNkunkuma, kwaye inikezelwe kwiinkampani ezinobungozi ezinobungozi.

Indlela nganye yokucoca inezibonelelo ezihambelanayo nezingalunganga. Eyona ndlela ilungileyo kukutshintsha i-vial entsha emva kovavanyo ngalunye. Kubalulekile ukuba ukhethe indlela yokucoca efanelekileyo. Ngokusekwe kuhlobo lwesampulu yovavanyo kunye neemeko zelebhu yokwenene, olu khetho kufuneka lwenziwe. Kuyacetyiswa nokuba kujongwane nokuqwalasela indleko zetyala. Kuya kufuneka ikhunjulwe ukuba ilandela uvavanyo ngalunye lweChromatographic, iSeptum yesampulu kufuneka itshintshwe.

I-Wan Ukufumana ulwazi olupheleleyo malunga nendlela yokucoca i-vialsgraphy yesampulu ye-chromatography, nceda ujonge eli nqaku: Iyasebenza kakuhle! Iindlela ezi-5 zokucoca iimodeli ze-chromatographys

Ngaphambili:

Khetha i-HPLC efanelekileyo ye-HPLC yokwandisa uhlalutyo lwakho lwe-HPPC

Okulandelayo:

Ngaba uyakuqonda ukulawulwa komgangatho we-HPLC?