Kwi-chemistry yohlalutyo, i-HPLC yindlela ephambili. Iyahlula-hlula, ichonge, kwaye isebenzise iinxalenye zomxube. Ukuchaneka kohlalutyo lwe-HPLC akuxhomekekanga ekutyhutyweni kwenkqubo. Ixhomekeke kumgangatho kwaye ilungele izinto ezisetyenziswayo ezisetyenzisiweyo. Oku kuyinyani ngakumbi kwi-vials ye-HPLC.

Indima ebalulekileyo ye-vials ye-HPLC

I-HPLC Vialszezona zikhongozeli ziphambili kwisicelo sesampulu. Bakwabeka iisampulu kwinkqubo yeChromatography. Ukukhetha i-val ayiqhelekanga. Sisigqibo esinokuchaphazela ukuchaneka kwesiphumo kunye nokuthembeka. Iivileni kufuneka zigcine iisampulu ezicocekileyo. Kuya kufuneka bayeke ukungcolisa. Kwaye, kufuneka basikelele kwi-HLLC.

Ufuna ukufunda yonke into malunga ne-vials ye-HPLC? Jonga eli nqaku libanzi:I-Encyclopedia ye-vials ye-HPLC.

Ukubaluleka kokukhula kwesampulu

Ingqibelelo yesampulu ibaluleke kakhulu kuhlalutyo lwe-HPPC. Iisampulu zigcinwa ngaphambi kohlalutyo zinokuba nefuthe iziphumo. Ukugcina okungalunganga kunokukhokelela kwimonakalo yesampula. Inokubangela ungcoliseko okanye uguqulelo. Le micimbi inokwenzakalisa ukuchaneka kohlalutyo. Ke, kubalulekile ukukhetha i-HLLC Vials. Kufuneka babonelele ngokugcina izinzile kunye ne-inter.

Ukuchaneka kweSampulu

Ukulungiselela iisampulu kubalulekile. Makwenziwe ngaphambi kokuba ivezi yazo ikwinkqubo ye-HPPC. Le nkqubo ibandakanya i-dilution, ukuxuba, kunye nokuzimela. Kufuneka kwenziwe ngokuchanekileyo ngokuchanekileyo ukuthintela ukongeza umahluko kuhlalutyo. I-vials elungileyo ye-HLLC iphambili. Bayeka ukusebenzisana phakathi kwesampulu kunye nezinto ze-vial. Unxibelelwano olunjalo lunokutshintsha isampulu.

Isampulu yesampulu yokuchaneka

Ukuchaneka kwenaliti yesampula sisimiselo esiphambili sokuthembeka kweziphumo ze-HPLC. Isampulu yesampulu kwinkqubo yeChromatography kufuneka ichaneke. Imele ifane nexesha ngalinye. I-vials ephezulu ye-HPLC yenzelwe ukugcina isampulu yesampulu iyafana. Uyilo lwe-Vic kufuneka luhambelane nee-ates ze-autosamselar.

Ndizibuza ukuba ungayicoca njani i-vials yakho? Jonga eli nqaku lichaza:Iindlela ezintlanu zokucoca ze-vials ze-HPLC.

Ukukhetha i-HPPC

Ukukhetha nokusebenzisa i-HLLC ibalulekile. Baqinisekisa ukuchaneka kunye nokuthembeka kweziphumo zeChromatographic. Ukukhetha i-Vial eLungileyo esezantsi komngcipheko wongcoliseko lwesampulu. Ikwathintela ilahleko yesampulu. Oku kugcina iziphumo ziyahambelana kwaye ziphinde zivelise.

Iipropathi zeekhemikhali zesampulu

Ubume bemichiza yesampulu buyalela ukhetho lwezinto eziphilayo. Iisampulu ze-acidic okanye i-alkaline zinokulungisa i-vials yeglasi. Oku kuthetha ukuba kufuneka usebenzise i-vials zeplastiki okanye i-vials yeglasi edityanisiweyo ukuba ixhaphake. Kwiisampulu ezinemihla ye-organic, izixhobo ze-Vial kufuneka zixhathise ezi zinyikima. Oku kuthintela ukukhutshwa okanye ukuphendula.

IIMFUNO ZOKUGQIBELA

Ukukhetha i-Vika kunokuba nefuthene. Oku kuhlalutya ngexabiso elincinci, njengoomaskishi okanye iintsalela zamachiza. Ii-vials ze-SILADDS zinokunciphisa isampulu yesampulu kwiindonga zangaphakathi ze-vial. Oku kuphucula iimvakalelo zokuchonga.I-Pre-captim septumI-Vials inqumle ilahleko yesampulu ngexesha lenaliti, iqinisekisa uhlalutyo oluchanekileyo.

Ngaba ungaqinisekanga ukuba ukhetha i-pre-slit septa okanye hayi i-vials yakho ye-HPPC? Jonga eli nqaku:Ungayikhetha njani iSepta: i-PAP-SLIT okanye hayi?

Inkqubo yenkqubo ye-HLLC

Ubungakanani kunye nohlobo lokuvula lwe-vial kufuneka luhambelane nejelo lenkqubo ye-HPPC. Ii-vials ezinkulu zomlomo zilungele iisampulu ezinzima. Iluncedo nakwisampuli equkethe amasuntswana aqinileyo. Kunciphisa umngcipheko wokufumana i-ockepy ngexesha lenaliti. Iibhotile ezivulekileyo ezivulekileyo zilungele ukusetyenziswa kwemihla ngemihla.

Ukuhambela phambili kwezobugcisa kwimveliso ye-Vial

Izazinzulu zenze inkqubela phambili yetekhnoloji kwimveliso ye-HPLC. Abavelisi ngoku babonelela ngeebhotile ezinepropathi ephuculweyo. Oku kubandakanya i-adsorption esezantsi,Ukuvuselela okuphezulu, kwayeI-Pre-Slit Septa. Ezi dilesi mtshathelwayo kwiimfuno eziguqukayo zeelebhu zohlalutyo. Yenza uhlalutyo oluchanekileyo noluchanekileyo.

Imilinganiselo ye-HPLC ye-HLLC ye-HLLC kunye neempawu

Abavelisi bemiGangatho iMgaba ye-HPLC ye-HPLC yokuqinisekisa ukuhambelana neentlobo ezahlukeneyo ze-autosamplers.

1. I-8x40mm (1ml) i-vials, rhoqo ngeCAPS yeCarw, ilungele imiqulu emincinci yesampulu.

I-2. Ii-vials zeziI-12x32mm (2ml) i-vials, ubungakanani obuqhelekileyo. Ziyafumaneka nge-screw caps okanye i-caps zoxinzelelo. I-CAPS yeyokuvula umgangatho okanye ngokubanzi.

3. I-15x45mm (4ml) i-vials, yenzelwe imiqulu emikhulu yesampula, idla ngokuvula ngokubanzi.

Izixhobo ze-HPLC

I-vials ye-HPLC yenziwe ngeglasi eqhelekileyo, iplastiki, kunye nezinto ezikhethekileyo. Umzekelo yiglasi edityanisiweyo ye-polymer, ekongeza uzinzo kunye nokuqina. Ukusetyenziswa okuthile, ngohlalutyo lweprotein,i-vials yeplastikiSika isampulu yesampulu kunye nokuphefumla. Uninzi lwe-HLLC oluqhelekileyo lwe-HLLC kunye ne-LC-ms ithatha i-vials yeglasi. Kungenxa yokuba bazinzile. I-Vials zeplastiki zilungile kwiisampuli ezingadingekiyo zibubushushu obuphakamileyo. Bakukhanya kwaye bancinci ukuba baphule. I-vials ye-SILADDS inonyango olukhethekileyo. Inyusa ubuntununtunu bokusetyenziswa ngokuthe ngqo.

Ukutywina i-vial

Ukutywinwa ngokuchanekileyo kwe-HPLC ye-HPLC kubalulekile ukuthintela isampulu kunye nongcoliseko. I-screw cap i-vials inesiphango sovavanyo olwenziwa rhoqo kwiinguqu rhoqo. I-Vials ene-cinezela-kwi-CAPS inetywina elikhuselekileyo. Zilungile kugcino lwexesha elide okanye ezothutho. Ukunqunyulwa kwangaphambili kwe-septum kwenziwa ukunceda iinaliti ze-autosampler zingene. Banquma umngcipheko wesampulu yokungcola kunye nelahleko.

Ngaba ucinga ukuba zeziphi izinto ekufuneka ziqwalaselwe xa ukhetha i-vial ye-automampler? Jonga eli nqaku lichaza:Iimpawu ezi-5 ekufuneka ziqwalaselwe xa ukhetha i-vial vial vial.

Ukuqukumbela

Ukukhetha nokusebenzisa i-vials ye-HLLC kubalulekile. Ziphambili kwimpumelelo ye-chromatography. Ukukhetha i-vials efanelekileyo sisitshixo. Kuya kufuneka batshatise iichemicals zesampulu, iimfuno zemvakalelo, kunye nenkqubo ye-HLPC. Ukwenza oku kunceda abaphengululi baphucula ukuchaneka kunye nokuthembakala kweziphumo zazo. Ukuhlawula ingqalelo kwiinkcukacha ekukhetheni i-Vial sisitshixo. Ibonisa indima ebaluleke kakhulu ye-HLLC ekufezekiseni ukugqwesa.

Ngaphambili:

Intsebenzo engalinganiyo yokutywina: Indlela yokungahambelani ne-Septa Ifuthe lePhantsi

Okulandelayo:

Khetha i-HPLC efanelekileyo ye-HPLC yokwandisa uhlalutyo lwakho lwe-HPPC